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Anti-Proteasome 26S S2/PSMD2抗体(ab26078)

Reviews (1) Submit a question References (1)
Western blot - Anti-Proteasome 26S S2/PSMD2 antibody (ab26078)
  • Immunocytochemistry/ Immunofluorescence - Anti-Proteasome 26S S2/PSMD2 antibody (ab26078)
  • Flow Cytometry (Intracellular) - Anti-Proteasome 26S S2/PSMD2 antibody (ab26078)

Key features and details

  • Goat polyclonal to Proteasome 26S S2/PSMD2
  • Suitable for: Flow Cyt (Intra), WB, ICC/IF
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

概述

  • 产品名称

    Anti-Proteasome 26S S2/PSMD2抗体
    参阅全部 Proteasome 26S S2/PSMD2 一抗

  • 描述

    山羊多克隆抗体to Proteasome 26S S2/PSMD2

  • 宿主

    Goat

  • 特异性

    ab26078 recognises Proteasome 26S, subunit S2 .

  • 经测试应用

    适用于: Flow Cyt (Intra), WB, ICC/IFmore details

  • 种属反应性

    与反应: Mouse, Rat, Human
    预测可用于: Pig, Macaque monkey

  • 免疫原

    Synthetic peptide corresponding to Human Proteasome 26S S2/PSMD2 aa 898-908 (C terminal).
    Sequence:

    C VILRKNPNYDL

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • 阳性对照

    • Flow Cyt (Intra): HeLa cell.

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • 存储溶液

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituent: 0.5% BSA
  • Concentration information loading...

  • 纯度

    Immunogen affinity purified

  • 克隆

    多克隆

  • 同种型

    IgG

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab26078于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Flow Cyt (Intra)
Use a concentration of 10 µg/ml.
WB (1)
Use a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 100 kDa.

1 hour primary incubation is recommended for this product.
ICC/IF
Use a concentration of 10 µg/ml.
说明
Flow Cyt (Intra)
Use a concentration of 10 µg/ml.
WB
Use a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 100 kDa.

1 hour primary incubation is recommended for this product.
ICC/IF
Use a concentration of 10 µg/ml.

靶标

  • 功能

    Acts as a regulatory subunit of the 26 proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins.
    Binds to the intracellular domain of tumor necrosis factor type 1 receptor. The binding domain of TRAP1 and TRAP2 resides outside the death domain of TNFR1.

  • 组织特异性

    Found in skeletal muscle, liver, heart, brain, kidney, pancreas, lung and placenta.

  • 序列相似性

    Belongs to the proteasome subunit S2 family.
    Contains 7 PC repeats.
  • Information by UniProt

  • 数据库链接

    see all

  • 别名

    • 26S proteasome non-ATPase regulatory subunit 2 antibody
    • 26S proteasome regulatory subunit RPN1 antibody
    • 26S proteasome regulatory subunit S2 antibody
    • 26S proteasome subunit p97 antibody
    • 55.11 protein antibody
    • MGC14274 antibody
    • P97 antibody
    • Proteasome (prosome macropain) 26S subunit non ATPase 2 antibody
    • Proteasome 26S subunit, non-ATPase, 2 antibody
    • Proteasome 26S, subunit 2 antibody
    • Protein 55.11 antibody
    • Psmd2 antibody
    • PSMD2_HUMAN antibody
    • Rpn1 antibody
    • S2 antibody
    • TNFR associated protein 2 antibody
    • TRAP2 antibody
    • Tumor necrosis factor receptor associated protein 2 antibody
    • Tumor necrosis factor type 1 receptor-associated protein 2 antibody
    see all

图片

  • Western blot - Anti-Proteasome 26S S2/PSMD2 antibody (ab26078)
    Western blot - Anti-Proteasome 26S S2/PSMD2 antibody (ab26078)
    Lanes 1-3 : Anti-Proteasome 26S S2/PSMD2 antibody (ab26078) at 1 µg/ml
    Lane 4 : Anti-Proteasome 26S S2/PSMD2 antibody (ab26078) at 0.3 µg/ml

    Lane 1 : HeLa cell lysate
    Lane 2 : U251 cell lysate
    Lane 3 : KNRK cell lysate
    Lane 4 : NIH3T3 cell lysate

    Lysates/proteins at 35 µg per lane.

    Predicted band size: 100 kDa



    Detected by chemiluminescence.

  • Immunocytochemistry/ Immunofluorescence - Anti-Proteasome 26S S2/PSMD2 antibody (ab26078)
    Immunocytochemistry/ Immunofluorescence - Anti-Proteasome 26S S2/PSMD2 antibody (ab26078)
    Immunocytochemistry/ Immunofluorescence analysis of HeLa cell labeling proteasome 26S S2/PSMD2 with ab26078 at 10 µg/mL followed by Alexa Fluor 488 secondary antibody (2µg/mL). Cells were fixed with paraformaldehyde and permeabilized with 0.15% Triton. The nuclei were stained with DAPI (blue). Negative control: Unimmunized goat IgG (10µg/mL) followed by Alexa Fluor 488 secondary antibody (2µg/mL).

     Cytoplasmic and some nuclear staining is observed.

  • Flow Cytometry (Intracellular) - Anti-Proteasome 26S S2/PSMD2 antibody (ab26078)
    Flow Cytometry (Intracellular) - Anti-Proteasome 26S S2/PSMD2 antibody (ab26078)
    Flow cytometry analysis of HeLa cell labeling proteasome 26S S2/PSMD2 with ab26078 at 10 µg/mL (blue) followed by Alexa Fluor 488 secondary antibody (1µg/mL) (blue). Cells were fixed with paraformaldehyde and permeabilized with 0.5% Triton. 
     
    IgG control: Unimmunized goat IgG (black) followed by Alexa Fluor 488 secondary antibody.

     

文献 (1)

发表研究结果有使用 ab26078？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab26078 被引用在 1 文献中.

  • Wang S  et al. Weighted gene co-expression network analysis reveals the hub genes associated with pulmonary hypertension. Exp Biol Med (Maywood) 248:217-231 (2023). PubMed: 36740764

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Western blot abreview for Anti-Proteasome 26S S2 antibody

 Good
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (NIH 3T3 fibroblasts)
Loading amount
25 µg
Specification
NIH 3T3 fibroblasts
Gel Running Conditions
Reduced Denaturing (10% Bis Tris)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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提交于 Feb 18 2010

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