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Cell Biology Proteolysis / Ubiquitin Proteasome / Ubiquitin Proteasome

Anti-Proteasome 20S LMP2抗体(ab3328)

  • Datasheet
Reviews (5)Q&A (1)References (31)

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Western blot - Anti-Proteasome 20S LMP2 antibody (ab3328)
  • Western blot - Anti-Proteasome 20S LMP2 antibody (ab3328)

Key features and details

  • Rabbit polyclonal to Proteasome 20S LMP2
  • Suitable for: WB
  • Reacts with: Mouse, Human
  • Isotype: IgG

选择批间可重复性更高的重组抗体

Product image
Anti-Proteasome 20S LMP2 antibody [EPR22042] (ab242061)
  • 研究可靠 —— 各批次间结果一致且可重复
  • 长期批量供应 —— 采用重组技术,可实现快速生产
  • 首次实验即可成功 —— 经过大量验证确认了特异性
  • 符合伦理标准 —— 产品不含动物成分

概述

  • 产品名称

    Anti-Proteasome 20S LMP2抗体
    参阅全部 Proteasome 20S LMP2 一抗
  • 描述

    兔多克隆抗体to Proteasome 20S LMP2
  • 宿主

    Rabbit
  • 经测试应用

    适用于: WBmore details
  • 种属反应性

    与反应: Mouse, Human
    预测可用于: Rat, Cow
  • 免疫原

    Synthetic peptide corresponding to Human Proteasome 20S LMP2 aa 206-219.
    Sequence:

    RVILGNELPKFYDE


    Database link: P28065
    (Peptide available as ab4944)
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 阳性对照

    • WB: HeLa cells, mouse embryo fibroblast cells
  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteasome / Ubiquitin
    • Proteasome
    • Neuroscience
    • Processes

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human Proteasome 20S LMP2 protein (ab105592)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab3328于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB (5)
Use a concentration of 1 µg/ml. (worked at 1/2000 (PMID 17114438))
说明
WB
Use a concentration of 1 µg/ml. (worked at 1/2000 (PMID 17114438))

靶标

  • 功能

    The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit is involved in antigen processing to generate class I binding peptides. Replacement of PSMB6 by PSMB9 increases the capacity of the immunoproteasome to cleave model peptides after hydrophobic and basic residues.
  • 序列相似性

    Belongs to the peptidase T1B family.
  • 发展阶段

    Highly expressed in immature dendritic cells (at protein level).
  • 翻译后修饰

    Autocleaved. The resulting N-terminal Thr residue of the mature subunit is responsible for the nucleophile proteolytic activity.
  • 细胞定位

    Cytoplasm. Nucleus.
  • Target information above from: UniProt accession P28065 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 510593 Cow
    • Entrez Gene: 5698 Human
    • Entrez Gene: 16912 Mouse
    • Entrez Gene: 24967 Rat
    • Omim: 177045 Human
    • SwissProt: Q3SZC2 Cow
    • SwissProt: P28065 Human
    • SwissProt: P28076 Mouse
    • SwissProt: P28077 Rat
    • Unigene: 654585 Human
    • Unigene: 390983 Mouse
    • Unigene: 13686 Rat
    see all
  • 别名

    • Beta1i antibody
    • Large multifunctional peptidase 2 antibody
    • Large multifunctional protease 2 antibody
    • LMP 2 antibody
    • LMP2 antibody
    • Low molecular mass protein 2 antibody
    • Macropain chain 7 antibody
    • MGC70470 antibody
    • Multicatalytic endopeptidase complex chain 7 antibody
    • OTTHUMP00000062982 antibody
    • Proteasome (prosome macropain) subunit beta type 9 antibody
    • proteasome (prosome, macropain) subunit, beta type, 9 (large multifunctional peptidase 2) antibody
    • Proteasome beta 9 subunit antibody
    • Proteasome catalytic subunit 1i antibody
    • Proteasome chain 7 antibody
    • Proteasome related gene 2 antibody
    • Proteasome subunit beta 6i antibody
    • Proteasome subunit beta type 9 antibody
    • Proteasome subunit beta type-9 antibody
    • Proteasome subunit beta-1i antibody
    • PSB9_HUMAN antibody
    • PSMB 9 antibody
    • PSMB6i antibody
    • PSMB9 antibody
    • Really interesting new gene 12 protein antibody
    • RING 12 antibody
    • RING12 antibody
    • RING12 protein antibody
    see all

图片

  • Western blot - Anti-Proteasome 20S LMP2 antibody (ab3328)
    Western blot - Anti-Proteasome 20S LMP2 antibody (ab3328)
    All lanes : Anti-Proteasome 20S LMP2 antibody (ab3328) at 2 µg/ml

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) treated with 100 ng/mL IFN gamma for 72 hours, whole cell lysate
    Lane 3 : THP-1 (human monocytic leukemia cell line) whole cell lysate
    Lane 4 : THP-1 (human monocytic leukemia cell line) treated with 50 ng/mL PMA for 48 hours and 200 ng/mL LPS for 24 hours, whole cell lysate
    Lane 5 : U937 (human histiocytic lymphoma cell line) whole cell lysate
    Lane 6 : Raji (human Burkitt's lymphoma cell line) whole cell lysate
    Lane 7 : Ramos (human Burkitt's lymphoma cell line) whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG (H+L) HRP conjugate at 0.4 µg/ml

    Developed using the ECL technique.
  • Western blot - Anti-Proteasome 20S LMP2 antibody (ab3328)
    Western blot - Anti-Proteasome 20S LMP2 antibody (ab3328)

    Western blot of proteasome 20S LMP2 in 50 ug mouse embryo fibroblast cell extract using ab3328.

实验方案

  • Western blot protocols
  • Immunohistochemistry protocols

Click here to view the general protocols

数据表及文件

  • Datasheet download

    Download

文献 (31)

发表研究结果有使用 ab3328?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab3328 被引用在 31 文献中.

  • Goetzke CC  et al. Mitigated viral myocarditis in A/J mice by the immunoproteasome inhibitor ONX 0914 depends on inhibition of systemic inflammatory responses in CoxsackievirusB3 infection. Basic Res Cardiol 116:7 (2021). PubMed: 33523326
  • Morotti M  et al. Increased expression of glutamine transporter SNAT2/SLC38A2 promotes glutamine dependence and oxidative stress resistance, and is associated with worse prognosis in triple-negative breast cancer. Br J Cancer 124:494-505 (2021). PubMed: 33028955
  • Kalaora S  et al. Immunoproteasome expression is associated with better prognosis and response to checkpoint therapies in melanoma. Nat Commun 11:896 (2020). PubMed: 32060274
  • Dang M  et al. Interferon-? mediates the protective effects of soluble receptor for advanced glycation end-product in myocardial ischemia/reperfusion. Lab Invest 99:358-370 (2019). PubMed: 30089851
  • Lee MJ  et al. H727 cells are inherently resistant to the proteasome inhibitor carfilzomib, yet require proteasome activity for cell survival and growth. Sci Rep 9:4089 (2019). PubMed: 30858500
View all Publications for this product

客户评价及客户问答

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1-6 of 6 Abreviews or Q&A

Western blot abreview for Anti-Proteasome 20S LMP2 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (THP-1 cells)
Gel Running Conditions
Reduced Denaturing (4 - 12%)
Loading amount
50000 cells
Specification
THP-1 cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 May 21 2019

Western blot abreview for Anti-Proteasome 20S LMP2 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (4-15%)
Sample
Human Cell lysate - whole cell (Lymphoma)
Specification
Lymphoma
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Dr. Satyendra Tripathi

Verified customer

提交于 Oct 07 2014

Western blot abreview for Anti-Proteasome 20S LMP2 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (dendritic cell)
Loading amount
1e+006 cells
Specification
dendritic cell
Gel Running Conditions
Non-reduced Denaturing
Blocking step
BSA as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Jan 05 2010

Western blot abreview for Anti-Proteasome 20S LMP2 antibody

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Purified protein (heart and spleen)
Loading amount
1 µg
Specification
heart and spleen
Gel Running Conditions
Reduced Denaturing (12,5%)
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Sep 03 2008

Western blot abreview for Anti-Proteasome 20S LMP2 antibody

Average
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (B-LCL)
Loading amount
80000 cells
Specification
B-LCL
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 18°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Jun 25 2007

Question

Please, could you give me informations about your antibodies against LMP2 catalog number ab 3328 and LMP7 catalog number ab 3329. I would like to know their reactivities (species...), their isotypes, their price and the modality to order them in France.

Read More

Abcam community

Verified customer

Asked on Oct 13 2003

Answer

Dear Anne All the information you have requested, including price, is available on the on line datasheets. I shall send them to you by e-mail also. You can order through the UK directly or through one of our distributors. Please use the ordering section on the top left hand side of the Abcam website. Best regards, Tracy Tracy Humphries New Products Assistant Abcam https://www.abcam.com/

Read More

Asdf Edo

Abcam Scientific Support

回复于 Oct 13 2003

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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