重组Anti-Proteasome 20S C2/HC2抗体[EPR5451] (ab109500)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome 20S C2/HC2 antibody [EPR5451] (ab109500)](https://www.abcam.cn/ps/products/109/ab109500/Images/Proteasome-20S-C2-Primary-antibodies-ab109500-4.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome 20S C2/HC2 antibody [EPR5451] (ab109500)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5451] to Proteasome 20S C2/HC2
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Proteasome 20S C2/HC2抗体[EPR5451]
参阅全部 Proteasome 20S C2/HC2 一抗 -
描述
兔单克隆抗体[EPR5451] to Proteasome 20S C2/HC2 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details
不适用于: Flow Cyt -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- HeLa, Jurkat, HepG2, and PC-3 cell lysates Human breast tissue
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR5451 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab109500于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 30 kDa.
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| IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Perform antigen retrieval |
| 说明 |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 30 kDa. |
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IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Perform antigen retrieval |
应用说明
Is unsuitable for Flow Cyt.
靶标
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功能
The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. Mediates the lipopolysaccharide-induced signal transduction in the macrophage proteasome (By similarity). Might be involved in the anti-inflammatory response of macrophages during the interaction with C.albicans heat-inactivated cells. -
序列相似性
Belongs to the peptidase T1A family. -
细胞定位
Cytoplasm. Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 5682 Human
- Entrez Gene: 26440 Mouse
- Entrez Gene: 29668 Rat
- Omim: 602854 Human
- SwissProt: P25786 Human
- SwissProt: Q9R1P4 Mouse
- SwissProt: P18420 Rat
- Unigene: 102798 Human
see all -
别名
- 30 kDa prosomal protein antibody
- HC 2 antibody
- HC2 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome 20S C2/HC2 antibody [EPR5451] (ab109500)
Immunohistochemical analysis of paraffin-embedded Human breast tissue using ab109500 at 1/250 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Western blot - Anti-Proteasome 20S C2/HC2 antibody [EPR5451] (ab109500)](https://www.abcam.cn/ps/products/109/ab109500/Images/Proteasome-20S-C2-Primary-antibodies-ab109500-1.jpg)
Western blot - Anti-Proteasome 20S C2/HC2 antibody [EPR5451] (ab109500)All lanes : Anti-Proteasome 20S C2/HC2 antibody [EPR5451] (ab109500) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : PC-3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 30 kDa -
![Anti-Proteasome 20S C2/HC2 antibody [EPR5451] (ab109500)](https://www.abcam.cn/ps/products/109/ab109500/Images/ab109500-3-benefits-of-recombinant-antibodies.png)
Anti-Proteasome 20S C2/HC2 antibody [EPR5451] (ab109500)
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab109500 被引用在 2 文献中.
- Wu S et al. Overexpression of RAB31 in gastric cancer is associated with released exosomes and increased tumor cell invasion and metastasis. Cancer Med 12:13497-13510 (2023). PubMed: 37222416
- Yang Q et al. Proteomic profiling of antibody-inducing immunogens in tumor tissue identifies PSMA1, LAP3, ANXA3, and maspin as colon cancer markers. Oncotarget 9:3996-4019 (2018). PubMed: 29423100