重组Anti-Proteasome 20S alpha 5/PSMA5抗体[EPR5832] (ab109387)
![Western blot - Anti-Proteasome 20S alpha 5/PSMA5 antibody [EPR5832] (ab109387)](https://www.abcam.cn/ps/products/109/ab109387/Images/ab109387-122183-anti-proteasome-20s-alpha-5-antibody-epr5832-western-blot.jpg "Western blot - Anti-Proteasome 20S alpha 5/PSMA5 antibody [EPR5832] (ab109387)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5832] to Proteasome 20S alpha 5/PSMA5
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Proteasome 20S alpha 5/PSMA5抗体[EPR5832]
参阅全部 Proteasome 20S alpha 5/PSMA5 一抗 -
描述
兔单克隆抗体[EPR5832] to Proteasome 20S alpha 5/PSMA5 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details
不适用于: IP -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: HepG2, 293T and JAR cell lysates. IHC-P: Human testis tissue
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解离常数（KD）
KD = 3.22 x 10 -11 M Learn more about KD -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR5832 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab109387于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 26 kDa.
|
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| IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.
|
| 说明 |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 26 kDa. |
|
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min. |
应用说明
Is unsuitable for IP.
靶标
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功能
The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. -
组织特异性
Expressed in fetal brain (at protein level). -
序列相似性
Belongs to the peptidase T1A family. -
细胞定位
Cytoplasm. Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 5686 Human
- Entrez Gene: 26442 Mouse
- Entrez Gene: 29672 Rat
- Omim: 176844 Human
- SwissProt: P28066 Human
- SwissProt: Q9Z2U1 Mouse
- SwissProt: P34064 Rat
- Unigene: 485246 Human
see all -
别名
- Macropain zeta chain antibody
- Multicatalytic endopeptidase complex zeta chain antibody
- Proteasome (prosome macropain) subunit alpha type 5 antibody
see all
图片
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Western blot - Anti-Proteasome 20S alpha 5/PSMA5 antibody [EPR5832] (ab109387)All lanes : Anti-Proteasome 20S alpha 5/PSMA5 antibody [EPR5832] (ab109387) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : 293T (Human embryonic kidney epithelial cell) cell lysate
Lane 3 : JAR cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 26 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome 20S alpha 5/PSMA5 antibody [EPR5832] (ab109387)](https://www.abcam.cn/ps/products/109/ab109387/Images/ab109387-122182-anti-proteasome-20s-alpha-5-antibody-epr5832-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome 20S alpha 5/PSMA5 antibody [EPR5832] (ab109387)Immunohistochemical analysis of paraffin-embedded Human testis tissue using 1/100 ab109387.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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![OI-RD Scanning - Anti-Proteasome 20S alpha 5/PSMA5 antibody [EPR5832] (ab109387)](https://www.abcam.cn/ps/products/109/ab109387/Images/ab109387-122181-anti-proteasome-20s-alpha-5-antibody-epr5832.jpg)
OI-RD Scanning - Anti-Proteasome 20S alpha 5/PSMA5 antibody [EPR5832] (ab109387) -
![Anti-Proteasome 20S alpha 5/PSMA5 antibody [EPR5832] (ab109387)](https://www.abcam.cn/ps/products/109/ab109387/Images/ab109387-4-benefits-of-recombinant-antibodies.png)
Anti-Proteasome 20S alpha 5/PSMA5 antibody [EPR5832] (ab109387)
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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