Anti-Proteasome 20S alpha 5/PSMA5抗体(ab11437)
Key features and details
- Rabbit polyclonal to Proteasome 20S alpha 5/PSMA5
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-Proteasome 20S alpha 5/PSMA5抗体
参阅全部 Proteasome 20S alpha 5/PSMA5 一抗 -
描述
兔多克隆抗体to Proteasome 20S alpha 5/PSMA5 -
宿主
Rabbit -
经测试应用
适用于: IHC-P, ICC/IF, WBmore details -
种属反应性
与反应: Human
预测可用于: Cow, Zebrafish
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免疫原
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.1% BSA -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab11437于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| ICC/IF |
Use a concentration of 1 µg/ml.
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| WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 26.5 kDa (predicted molecular weight: 26.5 kDa).
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| 说明 |
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IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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ICC/IF
Use a concentration of 1 µg/ml. |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 26.5 kDa (predicted molecular weight: 26.5 kDa). |
靶标
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功能
The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. -
组织特异性
Expressed in fetal brain (at protein level). -
序列相似性
Belongs to the peptidase T1A family. -
细胞定位
Cytoplasm. Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 510155 Cow
- Entrez Gene: 5686 Human
- Entrez Gene: 403011 Zebrafish
- Omim: 176844 Human
- SwissProt: Q5E987 Cow
- SwissProt: P28066 Human
- Unigene: 485246 Human
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别名
- Macropain zeta chain antibody
- Multicatalytic endopeptidase complex zeta chain antibody
- Proteasome (prosome macropain) subunit alpha type 5 antibody
see all
图片
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Western blot - Anti-Proteasome 20S alpha 5/PSMA5 antibody (ab11437)All lanes : Anti-Proteasome 20S alpha 5/PSMA5 antibody (ab11437)
Lane 1 : HeLa cell extract.
Lane 2 : HeLa cell extract with blocking peptide.
Predicted band size: 26.5 kDa
Observed band size: 26.5 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-Proteasome 20S alpha 5/PSMA5 antibody (ab11437)Immunofluorescence of HeLa cells using ab11437. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome 20S alpha 5/PSMA5 antibody (ab11437)IHC image of ab11437 staining in human liver carcinoma liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab11437, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (8)
ab11437 被引用在 8 文献中.
- Liu Q et al. The IFN-?-induced immunoproteasome is suppressed in highly pathogenic porcine reproductive and respiratory syndrome virus-infected alveolar macrophages. Vet Immunol Immunopathol 226:110069 (2020). PubMed: 32535163
- Sengupta D et al. Proteasomal degradation within endocytic organelles mediates antigen cross-presentation. EMBO J 38:e99266 (2019). PubMed: 31271236
- Wang C et al. Bone morphogenetic protein-2 exhibits therapeutic benefits for osteonecrosis of the femoral head through induction of cartilage and bone cells. Exp Ther Med 15:4298-4308 (2018). PubMed: 29849774
- Narcís JO et al. Accumulation of poly(A) RNA in nuclear granules enriched in Sam68 in motor neurons from the SMN?7 mouse model of SMA. Sci Rep 8:9646 (2018). PubMed: 29941967
- Mori F et al. Multitargeting activity of miR-24 inhibits long-term melatonin anticancer effects. Oncotarget 7:20532-48 (2016). WB . PubMed: 26967561
- Freudenburg W et al. Reduction in ATP levels triggers immunoproteasome activation by the 11S (PA28) regulator during early antiviral response mediated by IFNß in mouse pancreatic ß-cells. PLoS One 8:e52408 (2013). Mouse . PubMed: 23383295
- Ohta A et al. Herpes simplex virus type 1 UL14 tegument protein regulates intracellular compartmentalization of major tegument protein VP16. Virol J 8:365 (2011). ICC/IF . PubMed: 21791071
- de Verteuil D et al. Deletion of immunoproteasome subunits imprints on the transcriptome and has a broad impact on peptides presented by major histocompatibility complex I molecules. Mol Cell Proteomics 9:2034-47 (2010). WB ; Mouse . PubMed: 20484733