AB109387

重组Anti-Proteasome 20S alpha 5/PSMA5抗体[EPR5832]

Anti-Proteasome 20S alpha 5/PSMA5 antibody [EPR5832]

RabMAb
Recombinant
了解详情

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(2 Publications)

Rabbit Recombinant Monoclonal PSA5 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 2 publications.

查看别名

Proteasome subunit alpha type-5, Macropain zeta chain, Multicatalytic endopeptidase complex zeta chain, Proteasome subunit alpha-5, Proteasome zeta chain, alpha-5, PSMA5

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome 20S alpha 5/PSMA5 antibody [EPR5832] (AB109387)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome 20S alpha 5/PSMA5 antibody [EPR5832] (AB109387)

Immunohistochemical analysis of paraffin-embedded Human testis tissue using 1/100 ab109387.

Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

Unknown

Western blot - Anti-Proteasome 20S alpha 5/PSMA5 antibody [EPR5832] (AB109387)

All lanes:

Western blot - Anti-Proteasome 20S alpha 5/PSMA5 antibody [EPR5832] (ab109387) at 1/1000 dilution

Lane 1:

HepG2 cell lysate at 10 µg

Lane 2:

293T (Human embryonic kidney epithelial cell) cell lysate at 10 µg

Lane 3:

JAR cell lysate at 10 µg

Predicted band size: 26 kDa

false

OI-RD Scanning - Anti-Proteasome 20S alpha 5/PSMA5 antibody [EPR5832] (AB109387)

OI-RD Scanning

Unknown

OI-RD Scanning - Anti-Proteasome 20S alpha 5/PSMA5 antibody [EPR5832] (AB109387)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR5832

亚型

IgG

不含载体蛋白

反应种属

Human

应用

WB, IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

运输条件

Blue Ice

储存信息

Stable for 12 months at -20°C

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Proteasome 20S alpha 5 also known as PSMA5 is a subunit of the 20S proteasome core particle playing a role in protein degradation. This subunit weighs approximately 26 kDa and is expressed broadly across various tissues. PSMA5 forms part of the alpha ring of the proteasome and contributes to the gating mechanism controlling the entry of substrates into the catalytic core.

Biological function summary

PSMA5 functions within the multicatalytic proteinase complex known as the 20S proteasome. The proteasome is critical for degrading ubiquitinated proteins maintaining cellular protein homeostasis and regulating protein levels in the cell. By breaking down faulty or misfolded proteins and proteins involved in cell cycle regulation PSMA5 influences the turnover of proteins and ensures cellular function. PSMA5 is essential for cellular processes such as transcription cell cycle progression and apoptosis.

Pathways

PSMA5 has significant roles in both the ubiquitin-proteasome pathway and the NF-kB signaling pathway. In the ubiquitin-proteasome pathway PSMA5 helps degrade proteins tagged for destruction by ubiquitin involving components like ubiquitin-conjugating enzymes. In the NF-kB pathway the proteasome degrades inhibitors of NF-kB enabling the activation of this transcription factor and therefore facilitating gene expression related to immune and inflammatory responses.

PSMA5 has associations with cancer and neurodegenerative diseases. In cancer the overactivity of proteasome including PSMA5 can prevent the accumulation of pro-apoptotic factors promoting cell survival and tumor growth. Bortezomib a proteasome inhibitor targets this process to induce tumor cell death. In neurodegenerative diseases like Alzheimer's alterations in proteasome function may lead to the accumulation of toxic protein aggregates. Here PSMA5 interacts with proteins related to these conditions impacting disease progression and response to therapies.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. Associated with two 19S regulatory particles, forms the 26S proteasome and thus participates in the ATP-dependent degradation of ubiquitinated proteins. The 26S proteasome plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins that could impair cellular functions, and by removing proteins whose functions are no longer required. Associated with the PA200 or PA28, the 20S proteasome mediates ubiquitin-independent protein degradation. This type of proteolysis is required in several pathways including spermatogenesis (20S-PA200 complex) or generation of a subset of MHC class I-presented antigenic peptides (20S-PA28 complex).

See full target information Proteasome subunit alpha type-5

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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Immunity, inflammation and disease 12:e1146 PubMed38415977

2024

Knockdown of liver cancer cell-secreted exosomal PSMA5 controls macrophage polarization to restrain cancer progression by blocking JAK2/STAT3 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Shujie Xie,Xiang Li,Jia Yan,Hua Yu,Shuhuai Chen,Kana Chen

Nature communications 12:6829 PubMed34819513

2021

Nuclear-capture of endosomes depletes nuclear G-actin to promote SRF/MRTF activation and cancer cell invasion.

Applications

Unspecified application

Species

Unspecified reactive species

Sergi Marco,Matthew Neilson,Madeleine Moore,Arantxa Perez-Garcia,Holly Hall,Louise Mitchell,Sergio Lilla,Giovani R Blanco,Ann Hedley,Sara Zanivan,Jim C Norman

View all publications

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Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

重组Anti-Proteasome 20S alpha 5/PSMA5抗体[EPR5832]

Anti-Proteasome 20S alpha 5/PSMA5 antibody [EPR5832]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome 20S alpha 5/PSMA5 antibody [EPR5832] (AB109387)

Western blot - Anti-Proteasome 20S alpha 5/PSMA5 antibody [EPR5832] (AB109387)

OI-RD Scanning - Anti-Proteasome 20S alpha 5/PSMA5 antibody [EPR5832] (AB109387)

关键信息

宿主种属

克隆

克隆号

亚型

不含载体蛋白

反应种属

应用

免疫原