AB22674

Anti-Proteasome 20S alpha 1+2+3+5+6+7抗体[MCP231]

Name: Proteasome 20S alpha 1+2+3+5+6+7抗体[MCP231] (ab22674)| Abcam中文官网
Brand: Abcam Limited
SKU: AB22674
Price: 5045 CNY
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Rating: 5 (8 reviews)

Anti-Proteasome 20S alpha 1+2+3+5+6+7 antibody [MCP231]

(8 Reviews)

(33 Publications)

Anti-Proteasome 20S alpha 1+2+3+5+6+7 antibody [MCP231] (ab22674) is a mouse monoclonal antibody detecting Proteasome 20S alpha 1+2+3+5+6+7 in Flow Cytometry, ICC/IF. Suitable for Human.

- Over 20 publications
- Trusted since 2005

查看别名

HC2, NU, PROS30, PSC2, PSMA1, Proteasome subunit alpha type-1, 30 kDa prosomal protein, Macropain subunit C2, Multicatalytic endopeptidase complex subunit C2, Proteasome component C2, Proteasome nu chain, Proteasome subunit alpha-6, PROS-30, alpha-6

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-Proteasome 20S alpha 1+2+3+5+6+7 antibody [MCP231] (AB22674)

ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-Proteasome 20S alpha 1+2+3+5+6+7 antibody [MCP231] (AB22674)

ICC/IF image of ab22674 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab22674, 1μg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

This image was generated using the ascites version of the product.

Flow Cytometry - Anti-Proteasome 20S alpha 1+2+3+5+6+7 antibody [MCP231] (AB22674)

Flow Cyt

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Flow Cytometry - Anti-Proteasome 20S alpha 1+2+3+5+6+7 antibody [MCP231] (AB22674)

Overlay histogram showing HepG2 cells stained with ab22674 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab22674, 2μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was DyLight^® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed.

This image was generated using the ascites version of the product.

关键信息

宿主种属

Mouse

克隆

Monoclonal

克隆号

MCP231

亚型

IgG1

不含载体蛋白

反应种属

Human

应用

Flow Cyt, ICC/IF

applications

免疫原

Full Length Protein corresponding to Human PSMA1. The exact immunogen used to generate this antibody is proprietary information.

P25786

表位

Reacts with the peptide sequence TVWSPQGRLHQVEYAMEA encompassing the prosbox I motif common to alpha type subunits, although not necessarily identical in all. In general this motif is phylogenetically preserved.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "Does not immunoprecipitate intact proteasomes from HeLa cell preparations.", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<a href='/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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产品详情

What is this antibody validated in?
Anti-Proteasome 20S alpha 1+2+3+5+6+7 antibody [MCP231] (ab22674) is a mouse monoclonal antibody and is validated for use in Flow Cytometry (Flow Cyt), Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples.

Trusted by the scientific community
Anti-Proteasome 20S alpha 1+2+3+5+6+7 [MCP231] (ab22674) was first used in a scientific publication in 2005 and has been cited over 20 times in peer-reviewed journals.

Reviewed by scientists
Anti-Proteasome 20S alpha 1+2+3+5+6+7 [MCP231] (ab22674) has over 5 independent reviews from customers.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein G

存储溶液

Preservative: 0.01% Sodium azide Constituents: PBS

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The Proteasome 20S alpha subunits (also known as proteasome marker proscrate) are parts of the core structure of the 20S proteasome. This target features the six alpha subunits: alpha 1 alpha 2 alpha 3 alpha 5 alpha 6 and alpha 7. These alpha subunits collectively form the gate of the proteolytic chamber regulating substrate entry. The mass of the entire 20S proteasome complex is around 700 kDa. This target is ubiquitously expressed in eukaryotic cells playing a critical role in protein degradation.

Biological function summary

The 20S alpha subunit functions as part of the larger 26S proteasome complex which includes other components such as 19S regulatory particles. It facilitates protein turnover by degrading misfolded damaged or unneeded proteins into peptides. The 20S proteasome subunits' activity is essential in maintaining cellular homeostasis and controlling the concentration of specific proteins. They contribute to processes like cell cycle regulation and antigen presentation.

Pathways

The 20S proteasome involves itself directly in the ubiquitin-proteasome pathway a fundamental pathway for protein degradation. It is an important player in the immune response pathway aiding in processing peptides for major histocompatibility complex (MHC) class I antigen presentation. This function connects the 20S proteasome to other proteins such as ubiquitin and molecules involved in antigen processing.

Dysregulation of 20S proteasome activity associates with conditions such as cancer and neurodegenerative diseases. In cancer increased proteasome activity supports rapid cell division by degrading cell cycle inhibitors. Furthermore research links impaired proteasome function in neurodegenerative disorders such as Alzheimer's disease where inefficient protein degradation leads to the accumulation of toxic protein aggregates. The target connects to proteins like amyloid precursor protein in Alzheimer's emphasizing the critical role of proteasome subunits in disease pathogenesis.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. Associated with two 19S regulatory particles, forms the 26S proteasome and thus participates in the ATP-dependent degradation of ubiquitinated proteins. The 26S proteasome plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins that could impair cellular functions, and by removing proteins whose functions are no longer required. Associated with the PA200 or PA28, the 20S proteasome mediates ubiquitin-independent protein degradation. This type of proteolysis is required in several pathways including spermatogenesis (20S-PA200 complex) or generation of a subset of MHC class I-presented antigenic peptides (20S-PA28 complex).

See full target information PSMA1

其他靶点

PSMA2,PSMA3,Proteasome subunit alpha type-5,PSMA6,PSMA7

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文献 (33)

Recent publications for all applications. Explore the full list and refine your search

eLife 13: PubMed39230574

2024

Quantitative mapping of proteasome interactomes and substrates using ProteasomeID.

Applications

Unspecified application

Species

Unspecified reactive species

Aleksandar Bartolome,Julia C Heiby,Domenico Di Fraia,Ivonne Heinze,Hannah Knaudt,Ellen Spaeth,Omid Omrani,Alberto Minetti,Maleen Hofmann,Joanna M Kirkpatrick,Therese Dau,Alessandro Ori

PLoS genetics 20:e1011371 PubMed39083540

2024

Floxuridine supports UPS independent of germline signaling and proteostasis regulators via involvement of detoxification in C. elegans.

Applications

Unspecified application

Species

Unspecified reactive species

Abhishek Anil Dubey,Anwesha Sarkar,Karolina Milcz,Natalia A Szulc,Pankaj Thapa,Małgorzata Piechota,Remigiusz A Serwa,Wojciech Pokrzywa

Human molecular genetics 33:1506-1523 PubMed38776958

2024

PSMC5 insufficiency and P320R mutation impair proteasome function.

Applications

Unspecified application

Species

Unspecified reactive species

Zhong-Qiu Yu,Jenny Carmichael,Galen A Collins,Maria Daniela D'Agostino,Mathieu Lessard,Helen V Firth,Pooja Harijan,Andrew E Fry,John Dean,Jiuchun Zhang,Usha Kini,Alfred L Goldberg,David C Rubinsztein

Nature communications 14:4092 PubMed37433777

2023

Immunoproteasome-specific subunit PSMB9 induction is required to regulate cellular proteostasis upon mitochondrial dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

Minji Kim,Remigiusz A Serwa,Lukasz Samluk,Ida Suppanz,Agata Kodroń,Tomasz M Stępkowski,Praveenraj Elancheliyan,Biniyam Tsegaye,Silke Oeljeklaus,Michal Wasilewski,Bettina Warscheid,Agnieszka Chacinska

Frontiers in endocrinology 14:1176733 PubMed37201100

2023

The role of proteasome activators PA28αβ and PA200 in brown adipocyte differentiation and function.

Applications

Unspecified application

Species

Unspecified reactive species

Zeynep Koçberber,Nienke Willemsen,Alexander Bartelt

Research (Washington, D.C.) 6:0091 PubMed37223481

2023

SERBP1 Promotes Stress Granule Clearance by Regulating 26S Proteasome Activity and G3BP1 Ubiquitination and Protects Male Germ Cells from Thermostimuli Damage.

Applications

Unspecified application

Species

Unspecified reactive species

Fengli Wang,Lingjuan Wang,Shiming Gan,Shenglei Feng,Sijin Ouyang,Xiaoli Wang,Shuiqiao Yuan

Biomolecules 12: PubMed35740881

2022

Metformin Induces Resistance of Cancer Cells to the Proteasome Inhibitor Bortezomib.

Applications

Unspecified application

Species

Unspecified reactive species

Camille Schlesser,Thomas Meul,Georgios Stathopoulos,Silke Meiners

Molecular metabolism 62:101518 PubMed35636710

2022

Proteasome dysfunction disrupts adipogenesis and induces inflammation via ATF3.

Applications

Unspecified application

Species

Unspecified reactive species

Nienke Willemsen,Isabel Arigoni,Maja Studencka-Turski,Elke Krüger,Alexander Bartelt

Traffic (Copenhagen, Denmark) 23:287-304 PubMed35466519

2022

Extracellular 20S proteasome secreted via microvesicles can degrade poorly folded proteins and inhibit Galectin-3 agglutination activity.

Applications

Unspecified application

Species

Unspecified reactive species

Anne Bonhoure,Laurent Henry,Claudia Bich,Lionel Blanc,Blanche Bergeret,Marie-Pierre Bousquet,Olivier Coux,Pierre-Emmanuel Stoebner,Michel Vidal

The European respiratory journal 59: PubMed34561290

2022

Activation of immune cell proteasomes in peripheral blood of smokers and COPD patients: implications for therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Ilona E Kammerl,Sophie Hardy,Claudia Flexeder,Andrea Urmann,Julia Peierl,Yuqin Wang,Oliver Vosyka,Marion Frankenberger,Katrin Milger,Jürgen Behr,Andrea Koch,Juliane Merl-Pham,Stefanie M Hauck,Charles Pilette,Holger Schulz,Silke Meiners

View all publications

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Abcam Product Promise

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Anti-Proteasome 20S alpha 1+2+3+5+6+7抗体[MCP231]

Anti-Proteasome 20S alpha 1+2+3+5+6+7 antibody [MCP231]

Immunocytochemistry/ Immunofluorescence - Anti-Proteasome 20S alpha 1+2+3+5+6+7 antibody [MCP231] (AB22674)

Flow Cytometry - Anti-Proteasome 20S alpha 1+2+3+5+6+7 antibody [MCP231] (AB22674)

关键信息

宿主种属

克隆

克隆号

亚型

不含载体蛋白

反应种属

应用

免疫原

表位