AB190791

Anti-Protamine 2 抗体 [EPR15738]

Name: Protamine 2抗体[EPR15738] (ab190791)| Abcam中文官网
Brand: Abcam Limited
SKU: AB190791
Price: 841 CNY
Availability: InStock
Rating: 5 (1 reviews)

Anti-Protamine 2 antibody [EPR15738]

20ul selling size
RabMAb
Recombinant
了解详情

(1 Review)

(2 Publications)

Rabbit Recombinant Monoclonal Protamine 2 antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications.

查看别名

Protamine-2, Sperm histone P2, Sperm protamine P2, PRM2

1 Images

Supplier Data

Western blot - Anti-Protamine 2 antibody [EPR15738] (AB190791)

All lanes:

Western blot - Anti-Protamine 2 antibody [EPR15738] (ab190791) at 1/10000 dilution

All lanes:

Human testis lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 13 kDa

Observed band size: 17 kDa

false

不同偶联物与剂型 (1)

Carrier free

Anti-Protamine 2 antibody [EPR15738] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR15738

亚型

IgG

不含载体蛋白

反应种属

Human

应用

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

style

left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" } } }

style

left-column

产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Conditional Ambient

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

style

left-column

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Protamine 2 also known as PRM2 or protamine protein is a nuclear protein with a molecular mass of approximately 14 kDa. It is predominantly expressed in the testes specifically during the spermatogenesis process. Protamine 2 replaces histones in haploid phases of spermatids to ensure the proper condensation of sperm chromatin. Its structure comprises arginine-rich sequences enabling the tight packaging of DNA which is important for mature sperm functionality.

Biological function summary

Protamine 2 plays an essential role in sperm chromatin structure and stability interacting with other nucleoproteins to form a protamine-histone-DNA complex. This interaction is fundamental for the accurate packing of DNA within a sperm cell. Protamine 2 and its partners in the complex are critical for the fertility of males as they impact the genetic information's effective transmission to the next generation. The alteration of Protamine's balance affects the integrity of human sperm leading to reproductive challenges.

Pathways

Protamine 2 integrates into the spermatogenesis pathway coordinating with other proteins like Protamine 1 and transition proteins. Together they conduct transitions from histone-based chromatin to protamine-based packaging. This process is essential within the broader context of the reproductive signaling pathway which includes a range of post-translational modifications central to sperm development and maturation.

Disruptions in Protamine 2 expression link to male infertility as imbalances in Protamine 1 and 2 ratios can result in defective sperm packaging. Evidence also associates Protamine 2 dysregulation with a higher risk of sperm DNA fragmentation which can contribute to disorders such as recurrent pregnancy loss. The protein's performance and interaction with other proteins like histones and nuclear transition proteins point to its importance in maintaining genomic integrity during the paternal genetic material transmission.

style

left-column

style

left-column

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

靶点信息

Protamines substitute for histones in the chromatin of sperm during the haploid phase of spermatogenesis. They compact sperm DNA into a highly condensed, stable and inactive complex.

See full target information PRM2

style

left-column

文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Epigenetics 20:2498859 PubMed40296586

2025

Higher sperm H3K4me3 levels are associated with idiopathic recurrent pregnancy loss.

Applications

Unspecified application

Species

Unspecified reactive species

Delna Irani,Deepshikha Arya,Deepti Tandon,Vandana Bansal,Anushree Patil,Dipty Singh

Molecular & cellular proteomics : MCP 15:3297-3320 PubMed27451424

2016

Proteoform-Specific Insights into Cellular Proteome Regulation.

Applications

Unspecified application

Species

Unspecified reactive species

Emma L Norris,Madeleine J Headlam,Keyur A Dave,David D Smith,Alexander Bukreyev,Toshna Singh,Buddhika A Jayakody,Keith J Chappell,Peter L Collins,Jeffrey J Gorman

View all publications

style

left-column

style

left-column

style

right-column

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Protamine 2 抗体 [EPR15738]