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重组RabMAb

重组Anti-Prosurfactant蛋白C抗体[EPR19839] - BSA and Azide free (ab271991)

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  • Certificate of Compliance
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prosurfactant Protein C antibody [EPR19839] - BSA and Azide free (ab271991)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prosurfactant Protein C antibody [EPR19839] - BSA and Azide free (ab271991)
  • Immunohistochemistry (Frozen sections) - Anti-Prosurfactant Protein C antibody [EPR19839] - BSA and Azide free (ab271991)
  • Immunoprecipitation - Anti-Prosurfactant Protein C antibody [EPR19839] - BSA and Azide free (ab271991)
  • Anti-Prosurfactant Protein C antibody [EPR19839] - BSA and Azide free (ab271991)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19839] to Prosurfactant Protein C - BSA and Azide free
  • Suitable for: IP, IHC-Fr, IHC-P, WB
  • Reacts with: Mouse

Conjugates logo Related conjugates and formulations

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概述

  • 产品名称

    Anti-Prosurfactant蛋白C抗体[EPR19839] - BSA and Azide free
    参阅全部 Prosurfactant Protein C 一抗
  • 描述

    兔单克隆抗体[EPR19839] to Prosurfactant蛋白C - BSA and Azide free
  • 宿主

    Rabbit
  • 经测试应用

    适用于: IP, IHC-Fr, IHC-P, WBmore details
  • 种属反应性

    与反应: Mouse
  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • WB: Mouse lung lysate. IHC-P: Mouse lung tissue. IHC-Fr: Mouse lung tissue. IP: Mouse lung lysate.
  • 常规说明

    ab271991 is the carrier-free version of ab211326.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • 存储溶液

    pH: 7.2
    Constituent: PBS
  • 无载体

    是
  • Concentration information loading...
  • 纯度

    Protein A purified
  • 克隆

    单克隆
  • 克隆编号

    EPR19839
  • 同种型

    IgG
  • 研究领域

    • Cardiovascular
    • Lipids / Lipoproteins
    • Lipid Metabolism
    • Cholesterol Metabolism
    • Signal Transduction
    • Metabolism
    • Lipid metabolism
    • Cardiovascular
    • Vasculature
    • Vasculature Markers
    • Pulmonary
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of lipids and lipoproteins
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Lipid metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Cholesterol Metabolism

相关产品

  • Alternative Versions

    • Anti-Prosurfactant Protein C antibody [EPR19839] (ab211326)
    • HRP Anti-Prosurfactant Protein C antibody [EPR19839] (ab314245)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Related Products

    • VeriBlot for IP Detection Reagent (HRP) (ab131366)
    • Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (ab93684)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab271991于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IP
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.

Antigen retrieval: Heated citrate solution (10mM citrate PH 6.0 + 0.05% Tween-20).

IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
Use at an assay dependent concentration. Predicted molecular weight: 21 kDa.
说明
IP
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.

Antigen retrieval: Heated citrate solution (10mM citrate PH 6.0 + 0.05% Tween-20).

IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
Use at an assay dependent concentration. Predicted molecular weight: 21 kDa.

靶标

  • 功能

    Pulmonary surfactant associated proteins promote alveolar stability by lowering the surface tension at the air-liquid interface in the peripheral air spaces.
  • 疾病相关

    Defects in SFTPC are the cause of pulmonary surfactant metabolism dysfunction type 2 (SMDP2) [MIM:610913]; also called pulmonary alveolar proteinosis due to surfactant protein C deficiency. A rare disease associated with progressive respiratory insufficiency and lung disease with a variable clinical course, due to impaired surfactant homeostasis. It is characterized by alveolar filling with floccular material that stains positive using the periodic acid-Schiff method and is derived from surfactant phospholipids and protein components. Excessive lipoproteins accumulation in the alveoli results in severe respiratory distress.
    Genetic variations in SFTPC are a cause of susceptibility to respiratory distress syndrome in premature infants (RDS) [MIM:267450]; also known as RDS in prematurity. RDS is a lung disease affecting usually premature newborn infants. It is characterized by deficient gas exchange, diffuse atelectasis, high-permeability lung edema and fibrin-rich alveolar deposits called 'hyaline membranes'.
  • 序列相似性

    Contains 1 BRICHOS domain.
  • 细胞定位

    Secreted > extracellular space > surface film.
  • Target information above from: UniProt accession P11686 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 20389 Mouse
    • SwissProt: P21841 Mouse
    • Unigene: 24040 Mouse
    • 别名

      • BRICD6 antibody
      • BRICHOS domain containing 6 antibody
      • PSP C antibody
      • PSPC antibody
      • PSPC_HUMAN antibody
      • Pulmonary surfactant apoprotein 2 antibody
      • Pulmonary surfactant apoprotein PSP C antibody
      • pulmonary surfactant apoprotein-2 SP-C antibody
      • Pulmonary surfactant associated protein C antibody
      • Pulmonary surfactant associated proteolipid SPL pVal antibody
      • Pulmonary surfactant associated proteolipid SPL(Val) antibody
      • Pulmonary surfactant protein SP5 antibody
      • Pulmonary surfactant-associated protein C antibody
      • Pulmonary surfactant-associated proteolipid SPL(Val) antibody
      • SFTP 2 antibody
      • SFTP2 antibody
      • SFTPC antibody
      • SFTPC surfactant pulmonary associated protein C antibody
      • SMDP2 antibody
      • SP 5 antibody
      • SP C antibody
      • SP-C antibody
      • SP5 antibody
      • SPC antibody
      • Surfactant associated protein pulmonary 2 antibody
      • Surfactant protein c antibody
      • Surfactant proteolipid SPL-pVal antibody
      • Surfactant pulmonary associated protein C antibody
      see all

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prosurfactant Protein C antibody [EPR19839] - BSA and Azide free (ab271991)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prosurfactant Protein C antibody [EPR19839] - BSA and Azide free (ab271991)

      Immunohistochemical analysis of paraffin-embedded mouse lung tissue labeling Prosurfactant Protein C with ab211326 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Cytoplasmic staining on alveolar type II cells of mouse lung is observed [PMID: 15186480].

      Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab211326).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prosurfactant Protein C antibody [EPR19839] - BSA and Azide free (ab271991)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prosurfactant Protein C antibody [EPR19839] - BSA and Azide free (ab271991)

      Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling Prosurfactant Protein C with ab211326 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Negative control: no staining on mouse spleen. [PMID: 15186480]. Counter stained with Hematoxylin.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab211326).

    • Immunohistochemistry (Frozen sections) - Anti-Prosurfactant Protein C antibody [EPR19839] - BSA and Azide free (ab271991)
      Immunohistochemistry (Frozen sections) - Anti-Prosurfactant Protein C antibody [EPR19839] - BSA and Azide free (ab271991)

      Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse lung tissue labeling Prosurfactant Protein C with ab211326  at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

      Cytoplasmic staining on alveolar type II cells of mouse lung is observed [PMID: 23967208].

      The nuclear counterstain is DAPI (blue).

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab211326).

    • Immunoprecipitation - Anti-Prosurfactant Protein C antibody [EPR19839] - BSA and Azide free (ab271991)
      Immunoprecipitation - Anti-Prosurfactant Protein C antibody [EPR19839] - BSA and Azide free (ab271991)

      SP-C was immunoprecipitated from 0.35 mg of mouse lung lysate with ab211326 at 1/40 dilution.

      Western blot was performed from the immunoprecipitate using ab211326 at 1/1000 dilution.

      VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.

      Lane 1: Mouse lung lysate, 10ug (Input).

      Lane 2: ab211326 IP in mouse lung lysate.

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab211326 in mouse lung lysate.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 5 seconds.

      The banding pattern represents pro- and mature forms of SFTPC.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab211326).
    • Anti-Prosurfactant Protein C antibody [EPR19839] - BSA and Azide free (ab271991)
      Anti-Prosurfactant Protein C antibody [EPR19839] - BSA and Azide free (ab271991)

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    数据表及文件

    • Datasheet download

      Download

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    文献 (0)

    发表研究结果有使用 ab271991?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab271991 尚未被引用在任何文献中。

    客户评价及客户问答

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    Immunocytochemistry/ Immunofluorescence abreview for Anti-Prosurfactant Protein C antibody [EPR19839] - BSA and Azide free

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Lung)
    Permeabilization
    Yes - 0.5% Triton X-100 in TBS for 30 min
    Specification
    Lung
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 30µg/mL · Temperature: 25°C
    Fixative
    Frozen
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Jackson Lab

    Verified customer

    提交于 Feb 17 2022

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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