Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online

Anti-Prostate Specific Antigen抗体(ab9537)

Reviews (1)Q&A (9)References (7)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prostate Specific Antigen antibody (ab9537)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prostate Specific Antigen antibody (ab9537)

Key features and details

  • Rabbit polyclonal to Prostate Specific Antigen
  • Suitable for: IHC-Fr, IHC-P, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

概述

  • 产品名称

    Anti-Prostate Specific Antigen抗体
    参阅全部 Prostate Specific Antigen 一抗

  • 描述

    兔多克隆抗体to Prostate Specific Antigen

  • 宿主

    Rabbit

  • 经测试应用

    适用于: IHC-Fr, IHC-P, ICC/IFmore details

  • 种属反应性

    与反应: Human

  • 免疫原

    Full length native protein (purified) corresponding to Human Prostate Specific Antigen. Isolated from human seminal plasma.
    Database link: P07288

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab9537于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-Fr
Use at an assay dependent concentration.
IHC-P
1/50 - 1/150.
ICC/IF (1)
Use at an assay dependent concentration.
说明
IHC-Fr
Use at an assay dependent concentration.
IHC-P
1/50 - 1/150.
ICC/IF
Use at an assay dependent concentration.

靶标

  • 功能

    Hydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum.

  • 序列相似性

    Belongs to the peptidase S1 family. Kallikrein subfamily.
    Contains 1 peptidase S1 domain.

  • 细胞定位

    Secreted.
  • Information by UniProt

  • 数据库链接

    • 别名

      • antigen, prostate-specific antibody
      • APS antibody
      • Gamma seminoprotein antibody
      • Gamma-seminoprotein antibody
      • hK3 antibody
      • Kallikrein 3 antibody
      • Kallikrein related peptidase 3 antibody
      • Kallikrein-3 antibody
      • KLK 3 antibody
      • KLK2A1 antibody
      • Klk3 antibody
      • KLK3_HUMAN antibody
      • P-30 antigen antibody
      • P30 antigen antibody
      • Prostate-specific antigen antibody
      • Psa antibody
      • Semenogelase antibody
      • Seminin antibody
      see all

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prostate Specific Antigen antibody (ab9537)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prostate Specific Antigen antibody (ab9537)
      Formalin-fixed, paraffin-embeded human prostate carcinoma tissue stained for Prostate Specific Antigen using ab9537 at 1/50 dilution in immunohistochemical analysis. Antigen retrieval with citrate buffer pH 6.0.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prostate Specific Antigen antibody (ab9537)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prostate Specific Antigen antibody (ab9537)
      Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human prostate carcinoma tissue, staining Prostate Specific Antigen with ab9537. Staining was detected using DAB.

    文献 (7)

    发表研究结果有使用 ab9537？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab9537 被引用在 7 文献中.

    • Balint B  et al. Left atrial microvascular endothelial dysfunction, myocardial inflammation and fibrosis after selective insular cortex ischemic stroke. Int J Cardiol 292:148-155 (2019). PubMed: 31196685
    • Sanz G  et al. Structurally related odorant ligands of the olfactory receptor OR51E2 differentially promote metastasis emergence and tumor growth. Oncotarget 8:4330-4341 (2017). PubMed: 28032594
    • Sanz G  et al. Gallein, a Gß? subunit signalling inhibitor, inhibits metastatic spread of tumour cells expressing OR51E2 and exposed to its odorant ligand. BMC Res Notes 10:541 (2017). PubMed: 29084601
    • Sanz G  et al. Promotion of cancer cell invasiveness and metastasis emergence caused by olfactory receptor stimulation. PLoS One 9:e85110 (2014). IHC (PFA fixed) ; Mouse . PubMed: 24416348
    • Berry SM  et al. Streamlining immunoassays with immiscible filtrations assisted by surface tension. Anal Chem 84:5518-23 (2012). PubMed: 22632629
    • Drake RR  et al. Clinical collection and protein properties of expressed prostatic secretions as a source for biomarkers of prostatic disease. J Proteomics 72:907-17 (2009). PubMed: 19457353
    • Pettus JR  et al. Multiple kallikrein (KLK 5, 7, 8, and 10) expression in squamous cell carcinoma of the oral cavity. Histol Histopathol 24:197-207 (2009). IHC-P ; Human . PubMed: 19085836

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-10 of 10 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Prostate Specific Antigen antibody

     Good
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (LNCaP Cells)
    Specification
    LNCaP Cells
    Fixative
    Methanol
    Blocking step
    Milk as blocking agent for 10 minute(s) · Concentration: 10%
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Apr 16 2007

    Question

    I have been using this antibody for several years and the most recent lot is not working (no staining). I would like to try antibody from another lot.

    Read More
    Answer

    Thank you for contacting Abcam regarding ab9537.


    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement.

    To check the status of the order please contact our Customer Service team and reference this number.

    Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

    I wish you the best of luck with your research.

    Read More

    Question

    I have some short questions about your Anti-Prostate Specific antibody (ab9537)

    1) Can you asure that it works for IF? Has anybody ever published on that?

    3) Could you provide us with 300 microgramms of antibody? Could you send a quote for that amount?

    Thank you very much in advance


    Read More
    Answer

    Thank you for contacting Abcam regarding ab9537.


    This antibody has been validated for IF, however currently we do not have any references available in which a customer has published this data.


    I have forwarded your quote request to our sales department for additional help.


    I hope this information is helpful. Please do not hesitate to contact us if you have any other questions.

    Read More

    Question

    Dear technical support: This customer has highly interested in ab9537 (Anti-Prostate Specific Antigen antibody) and ab119154 (Prostate Specific Antigen protein), this customer would like to know can ab9537 recognize to ab119154 or not? If not, could you please offer this customer any antibody and antigen which can specific recognizing to each other? Could you please help this customer to solve the problem? Thanks for your kindly help. Best regards

    Read More
    Answer

    Thank you for your enquiry and interest.

    Could you please clarify and confirm in what type of assay/application your customer is looking for a primary antibody and antigen? This would be important for me to know to be able to suggest the best products for you and your customer.

    I look forward to hearing from you soon.

    Read More

    Question

    don't you agree that the information on the data sheet about the antibody concentration 1.5 mg/ml is wrong?

    Read More
    Answer

    Thank you for your email. I'm replying on behalf of Charlotte who is currently away from the office. To clarify the concentration of this product, the concentration of the anti-PSA IgG is 100 ug/ml. This antibody has not been affinity purified (the purity of the antibody is IgG fraction) and the 1.5 mg/ml is the concentration of all the IgGs present. The 1.5 mg/ml information on the datasheet is misleading and I have removed it. I apologize for any confusion. I hope this helps, please contact us again if you have any additional questions.

    Read More

    Question

    I would like to ask you to check the concentration information of AB9537 anti PSA antibody. Can you check whether it is indeed 1.5 mg/ml. I purified it using perce melon gel and found the rabbit IgG is only 100 ng/ml.

    Read More
    Answer

    Thank you for your enquiry. Following correspondence with the lab I have determined that the total protein conc. of this antibody is 1.5 mg/ml but the IgG conc. is 100 ug/ml. Please can you confirm that you mean 100 ng/ml and not 100 ug/ml.

    Read More

    Question

    Thanks for the reply. Unfortunately we cannot order a large amount. Do you mind give me the contact information of the antibody originator? I thought it would not be very difficult by NOT adding BSA. Second question: if it is impossible to get the above BSA-free antibody, how about the ab403 and ab2218. Do they contain BSA or any other proteins in their storage buffer?

    Read More
    Answer

    Thank you for your email. I can't tell you the name of the originator of ab9537 as that information is proprietary. I double-checked ab403 and ab2218, and both of these do not contain BSA or other proteins in the storage buffer. Ab403 consists of preservative: 0.1% Sodium Azide, and constituents: sodium chloride, citrate / phosphate. pH 6.0. Ab2218 consists of preservative: 15mM sodium azide, and constituents: PBS. pH 7.4. Please contact us again if you have any additional questions or concerns.

    Read More

    Question

    I measured the concentration of this antibody by spectral photometer and found the concentration is much higher than 1.5 mg/ml (it is around 5 mg/ml). Could you please double check it for me that this antibody has no any other proteins in the storage buffer? Thank you very much.

    Read More
    Answer

    Thank you for your email. I checked with the antibody's originator again and the antibody is suspended in PBS which contains BSA. I apologize as this information was not mentioned before. I have now added it to the online datasheet. Please contact us again if you have any additional questions.

    Read More

    Question

    Thank you very much. Does the antibody contains any serum, BSA or other proteins?

    Read More
    Answer

    Thank you for your email. The antibody storage buffer consists of PBS with 0.05% Sodium Azide. Please contact us again if you have any additional questions.

    Read More

    Question

    What is contained in the antibody stoarge buffer? Also, does this antibody stain glandular epithelium as well as ductal epithelium?

    Read More
    Answer

    Thank you for your enquiry. The antibody is suspended in PBS and stains ductal epithelium. We do not know if glandular epithelium is also stained. Please contact us again if you have any additional questions.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com