Anti-Prostate Specific Antigen抗体(ab53774)
Key features and details
- Rabbit polyclonal to Prostate Specific Antigen
- Suitable for: WB, IHC-P, ELISA
- Reacts with: Mouse, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Prostate Specific Antigen抗体
参阅全部 Prostate Specific Antigen 一抗 -
描述
兔多克隆抗体to Prostate Specific Antigen -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ELISAmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Synthetic peptide derived from human Prostate Specific Antigen
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab53774于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) |
1/500 - 1/1000. Detects a band of approximately 34 kDa (predicted molecular weight: 29 kDa).
|
| IHC-P |
Use at an assay dependent concentration.
|
|
| ELISA | (1) |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
WB
1/500 - 1/1000. Detects a band of approximately 34 kDa (predicted molecular weight: 29 kDa). |
|
IHC-P
Use at an assay dependent concentration. |
|
ELISA
Use at an assay dependent concentration. |
靶标
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功能
Hydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum. -
序列相似性
Belongs to the peptidase S1 family. Kallikrein subfamily.
Contains 1 peptidase S1 domain. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 354 Human
- Entrez Gene: 16621 Mouse
- Omim: 176820 Human
- SwissProt: P07288 Human
- SwissProt: P00756 Mouse
- Unigene: 171995 Human
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别名
- antigen, prostate-specific antibody
- APS antibody
- Gamma seminoprotein antibody
see all
图片
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Western blot - Anti-Prostate Specific Antigen antibody (ab53774)All lanes : Anti-Prostate Specific Antigen antibody (ab53774) at 1/500 dilution
Lane 1 : Extracts from mouse heart
Lane 2 : Extracts from mouse heart, plus immunising peptide
Predicted band size: 29 kDa
Observed band size: 34 kDa why is the actual band size different from the predicted? -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prostate Specific Antigen antibody (ab53774)Ab53774 staining human normal prostate tissue. Staining is localised to cytoplasm and extracellular spaces.
Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
Sections were stained using an automated system (DAKO Autostainer Plus ), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be requir
数据表及文件
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SDS download
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Datasheet download
文献 (15)
ab53774 被引用在 15 文献中.
- Wissmiller K et al. Inceptor correlates with markers of prostate cancer progression and modulates insulin/IGF1 signaling and cancer cell migration. Mol Metab 71:101706 (2023). PubMed: 36931467
- Shin WS et al. Pinostilbene inhibits full-length and splice variant of androgen receptor in prostate cancer. Sci Rep 13:16663 (2023). PubMed: 37794090
- Whitburn J et al. Metabolic profiling of prostate cancer in skeletal microenvironments identifies G6PD as a key mediator of growth and survival. Sci Adv 8:eabf9096 (2022). PubMed: 35213227
- Wang H et al. Lncap-AI prostate cancer cell line establishment by Flutamide and androgen-free environment to promote cell adherent. BMC Mol Cell Biol 23:51 (2022). PubMed: 36443669
- Franko A et al. Characterization of Hormone-Dependent Pathways in Six Human Prostate-Cancer Cell Lines: A Gene-Expression Study. Genes (Basel) 11:N/A (2020). PubMed: 33036464