重组Anti-proNGF抗体[EP1320Y] - BSA and Azide free (ab271847)
![Immunocytochemistry/ Immunofluorescence - Anti-proNGF antibody [EP1320Y] - BSA and Azide free (ab271847)](https://www.abcam.cn/ps/products/271/ab271847/Images/ab271847-4-ab52918243830antingfantibodyep1320yimmunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - Anti-proNGF antibody [EP1320Y] - BSA and Azide free (ab271847)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1320Y] to proNGF - BSA and Azide free
- Suitable for: ICC/IF, WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-proNGF抗体[EP1320Y] - BSA and Azide free
参阅全部 proNGF 一抗 -
描述
兔单克隆抗体[EP1320Y] to proNGF - BSA and Azide free -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, WBmore details
不适用于: IHC-P -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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常规说明
ab271847 is the carrier-free version of ab52918.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
存储溶液
pH: 7.20
Constituent: PBS -
无载体
是 -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP1320Y -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab271847于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
Use at an assay dependent concentration.
|
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| WB |
Use at an assay dependent concentration. Predicted molecular weight: 26 kDa.
For unpurified, use 1/500. |
| 说明 |
|---|
|
ICC/IF
Use at an assay dependent concentration. |
|
WB
Use at an assay dependent concentration. Predicted molecular weight: 26 kDa. For unpurified, use 1/500. |
靶标
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功能
Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades through those receptor tyrosine kinase to regulate neuronal proliferation, differentiation and survival. Inhibits metalloproteinase dependent proteolysis of platelet glycoprotein VI (PubMed:20164177). -
疾病相关
Neuropathy, hereditary sensory and autonomic, 5 -
序列相似性
Belongs to the NGF-beta family. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 4803 Human
- SwissProt: P01138 Human
- SwissProt: P01139 Mouse
- SwissProt: P25427 Rat
- Unigene: 2561 Human
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别名
- Beta-nerve growth factor antibody
- Beta-NGF antibody
- NGF antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-proNGF antibody [EP1320Y] - BSA and Azide free (ab271847)
Immunofluorescence staining of U87-MG cells with purified ab52918 at a working dilution of 1 in 300, counter-stained with DAPI. Tubulin was stained with mouse anti-tubulin at a dilution of 1/1000 (ab7291) and Alexa Fluor® 594 goat anti-mouse at a dilution of 1/500 (ab150120) . The secondary antibody was ab150077 Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1 in 500. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in the bottom middle and right hand panels - for the first negative control, purified ab52918 was used at a dilution of 1/200 followed by an Alexa Fluor® 555 goat anti-mouse antibody at a dilution of 1/500 and for the second negative control mouse primary antibody (ab7291) and anti-rabbit secondary antibody (ab15007) were used.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52918). -
![Anti-proNGF antibody [EP1320Y] - BSA and Azide free (ab271847)](https://www.abcam.cn/ps/products/271/ab271847/Images/ab271847-5-benefits-of-recombinant-antibodies.png)
Anti-proNGF antibody [EP1320Y] - BSA and Azide free (ab271847)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
Certificate of Compliance
文献 (0)
ab271847 尚未被引用在任何文献中。
