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Signal Transduction Protein Phosphorylation Tyrosine Kinases Receptor Tyrosine Kinases

Anti-Prolactin Receptor/PRL-R抗体[T6] (ab2773)

  • Datasheet
Reviews (2)Q&A (4)References (6)

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Immunocytochemistry/ Immunofluorescence - Anti-Prolactin Receptor/PRL-R antibody [T6] (ab2773)
  • Immunocytochemistry/ Immunofluorescence - Anti-Prolactin Receptor/PRL-R antibody [T6] (ab2773)
  • Immunocytochemistry/ Immunofluorescence - Anti-Prolactin Receptor/PRL-R antibody [T6] (ab2773)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prolactin Receptor/PRL-R antibody [T6] (ab2773)

Key features and details

  • Mouse monoclonal [T6] to Prolactin Receptor/PRL-R
  • Suitable for: IHC-P, ICC/IF
  • Reacts with: Rat, Human
  • Isotype: IgG2a

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概述

  • 产品名称

    Anti-Prolactin Receptor/PRL-R抗体[T6]
    参阅全部 Prolactin Receptor/PRL-R 一抗
  • 描述

    小鼠单克隆抗体[T6] to Prolactin Receptor/PRL-R
  • 宿主

    Mouse
  • 特异性

    Detects Prolactin Receptor/PRL-R in rat tissues. This antibody does not cross-react with Growth Hormone (GH) receptor. By Western blot, this antibody detects an ~42 kDa protein representing Prolactin Receptor/PRL-R in NB2 cell lysate. Immunohistochemical staining of Prolactin Receptor/PRL-R in NB2 cells yields a staining pattern consistent with cytoplasmic vesicular staining. This antibody has also been used to inhibit the binding of prolactin to Prolactin Receptor/PRL-R in vitro.

  • 经测试应用

    适用于: IHC-P, ICC/IFmore details
  • 种属反应性

    与反应: Rat, Human
  • 免疫原

    Full length native protein (purified) corresponding to Rat Prolactin Receptor/PRL-R. Purified rat liver PRL receptor.

  • 阳性对照

    • IHC-P: Rat pituitary gland tissue. ICC: C6, H-4-II-E, SW480 cells.
  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    Constituent: PBS
  • Concentration information loading...
  • 纯度

    Protein A purified
  • Primary antibody说明

    Prolactin (PRL) is a hormone involved in a variety of important functions including ion transport and osmoregulation, stimulation of milk, protein synthesis as well as the regulation of numerous reproductive functions. PRL exerts its influence on different cell types through a signal transduction pathway which begins with the binding of the hormone to a transmembrane PRL receptor. Immunoreactive PRL receptor, a member of the cytokine receptor family, varies in size (short and long forms) with tissue source and species, from ~40 kDa to 100 kDa. The PRL receptor consists of at least three separate domains: an extracellular region with 5 cysteines which contains the prolactin binding site, a single transmembrane domain and a cytoplasmic region, the length of which appears to influence ligand binding and regulate cellular function.
  • 克隆

    单克隆
  • 克隆编号

    T6
  • 同种型

    IgG2a
  • 研究领域

    • Signal Transduction
    • Protein Phosphorylation
    • Tyrosine Kinases
    • Receptor Tyrosine Kinases
    • Signal Transduction
    • Growth Factors/Hormones
    • Hormones
    • Signal Transduction
    • Metabolism
    • Lipid metabolism
    • Metabolism
    • Types of disease
    • Cancer

相关产品

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG2a, kappa monoclonal [MG2a-53] - Isotype control (ab18415)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab2773于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-P
1/25 - 1/200.
ICC/IF
1/200.
说明
IHC-P
1/25 - 1/200.
ICC/IF
1/200.

靶标

  • 功能

    This is a receptor for the anterior pituitary hormone prolactin (PRL). Isoform 4 is unable to transduce prolactin signaling. Isoform 6 is unable to transduce prolactin signaling.
  • 组织特异性

    Expressed in breast, placenta, kidney, liver and pancreas.
  • 序列相似性

    Belongs to the type I cytokine receptor family. Type 1 subfamily.
    Contains 2 fibronectin type-III domains.
  • 结构域

    The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
    The box 1 motif is required for JAK interaction and/or activation.
  • 细胞定位

    Secreted and Membrane.
  • Target information above from: UniProt accession P16471 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 5618 Human
    • Entrez Gene: 24684 Rat
    • Omim: 176761 Human
    • SwissProt: P16471 Human
    • SwissProt: P05710 Rat
    • Unigene: 368587 Human
    • Unigene: 9757 Rat
    • 别名

      • AI987712 antibody
      • CLONE SPM213 antibody
      • CPRLP antibody
      • Delta 4-delta 7/11 truncated prolactin receptor antibody
      • Delta 4-SF1b truncated prolactin receptor antibody
      • HPRL antibody
      • hPRL receptor antibody
      • hPRLrI antibody
      • Lactogen receptor antibody
      • MFAB antibody
      • MGC105486 antibody
      • OPR antibody
      • OTTHUMP00000115998 antibody
      • Pr-1 antibody
      • Pr-3 antibody
      • PRL R antibody
      • PRL-R antibody
      • PRLR antibody
      • Prlr-rs1 antibody
      • PRLR_HUMAN antibody
      • Prolactin receptor a antibody
      • Prolactin receptor antibody
      • Prolactin receptor delta 7/11 antibody
      • RATPRLR antibody
      • Secreted prolactin binding protein antibody
      • Truncated testis-specific box 1-C prolactin receptor antibody
      • wu:fj65c07 antibody
      see all

    图片

    • Immunocytochemistry/ Immunofluorescence - Anti-Prolactin Receptor/PRL-R antibody [T6] (ab2773)
      Immunocytochemistry/ Immunofluorescence - Anti-Prolactin Receptor/PRL-R antibody [T6] (ab2773)

      Immunocytochemical analysis of C6 (Rat glial tumor cell line) cells labeling Prolactin Receptor/PRL-R. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were incbated without (control) or with ab2773 (1:200) overnight at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Prolactin Receptor/PRL-R staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.

    • Immunocytochemistry/ Immunofluorescence - Anti-Prolactin Receptor/PRL-R antibody [T6] (ab2773)
      Immunocytochemistry/ Immunofluorescence - Anti-Prolactin Receptor/PRL-R antibody [T6] (ab2773)

      Immunocytochemical analysis of H-4-II-E (Rat hepatoma cell line) cells labeling Prolactin Receptor/PRL-R. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were incbated without (control) or with ab2773 (1:200) overnight at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Prolactin Receptor/PRL-R staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.

    • Immunocytochemistry/ Immunofluorescence - Anti-Prolactin Receptor/PRL-R antibody [T6] (ab2773)
      Immunocytochemistry/ Immunofluorescence - Anti-Prolactin Receptor/PRL-R antibody [T6] (ab2773)

      Immunocytochemical analysis of in SW480 (Human colorectal adenocarcinoma cell line) cells labeling Prolactin Receptor/PRL-R. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were incbated without (control) or with ab2773 (1:200) overnight at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Prolactin Receptor/PRL-R staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prolactin Receptor/PRL-R antibody [T6] (ab2773)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prolactin Receptor/PRL-R antibody [T6] (ab2773)

      Immunohistochemical analysis of normal biopsies of deparaffinized rat pituitary gland tissue labeling Prolactin Receptor/PRL-R. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature and incubated with ab2773 (1:50) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

    实验方案

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    数据表及文件

    • Datasheet download

      Download

    文献 (6)

    发表研究结果有使用 ab2773?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab2773 被引用在 6 文献中.

    • Dardenne M  et al. Prolactin receptor expression in human hematopoietic tissues analyzed by flow cytofluorometry. Endocrinology 134:2108-14 (1994). PubMed: 8156910
    • Rui H  et al. JAK2 activation and cell proliferation induced by antibody-mediated prolactin receptor dimerization. Endocrinology 135:1299-306 (1994). PubMed: 7925093
    • Gagnerault MC  et al. Expression of prolactin receptors in murine lymphoid cells in normal and autoimmune situations. J Immunol 150:5673-81 (1993). PubMed: 8515082
    • Dardenne M  et al. Identification and functional activity of prolactin receptors in thymic epithelial cells. Proc Natl Acad Sci U S A 88:9700-4 (1991). PubMed: 1946390
    • Rozakis-Adcock M & Kelly PA Mutational analysis of the ligand-binding domain of the prolactin receptor. J Biol Chem 266:16472-7 (1991). PubMed: 1885580
    • Okamura H  et al. Characterization and applications of monoclonal antibodies to the prolactin receptor. Endocrinology 124:2499-508 (1989). PubMed: 2707162

    客户评价及客户问答

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    1-6 of 6 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Prolactin Receptor/PRL-R antibody [T6]

    Inconclusive
    Abreviews
    Abreviews
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (liver, mammary gland)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citrate buffer ph 6.0
    Permeabilization
    No
    Specification
    liver, mammary gland
    Blocking step
    protein block as blocking agent for 10 minute(s) · Concentration: 100% · Temperature: rt°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MS. Francine Deslippe

    Verified customer

    提交于 Apr 10 2013

    Western blot abreview for Anti-Prolactin Receptor/PRL-R antibody [T6]

    Poor
    Abreviews
    Abreviews
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Forebrain)
    Loading amount
    40 µg
    Specification
    Forebrain
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Jan 09 2007

    Question

    ab25630: WB with rat liver
    sees band at 25 kDa
    transfer is OK, detects other proteins in the blot
    Ab: 1/1000 1-2 h RT
    block: overnight 4 degree
    secondary: OK
    antibody from other company to same protein gives same results
    suggestions: WB conditions might not yet be optimal, try blocking for only 1-2 h RT, antibody incubation: overnight 4 degree
    ab25631: rat is unsuitable
    ab2773: WB with rat liver, sees band at 25 kDa
    suggestions: WB conditions might not yet be optimal, try blocking for only 1-2 h RT, antibody incubation: overnight 4 degree

    Read More

    Abcam community

    Verified customer

    Asked on Feb 28 2012

    Answer

    Thank you for contacting us.
    I am sorry to hear about the problems you have encountered.

    The bands you are seeing at 25 kDa could be the light chain of endogenous IgG.

    As we discussed over the phone, please try the following suggestions as it seems the WB conditions for these 2 antibodies (ab25630 and ab2773) are not yet optimal:

    1) try blocking for only 1-2 h RT
    2) try the primary antibody incubation for overnight 4 degree

    As for ab25631, this has been shown to not react with rat samples and would probably not give satisfactory results.However, if you have access to a human sample, you could try the antibody on that.

    Please let me know if the tips were helping to improve your results.

    I hope this information is helpful to you, and I look forward to hear back.

    Read More

    Abcam Scientific Support

    回复于 Feb 28 2012

    Question

    Our customer has tried the proteolytic pretreatment with 0.1% trypsin but the result again was not very convincing (see attached pdf-file 'PrlR-Staining'). The file shows pictures taken from stainings with AB2773 (page2) and pictures taken from stainings with the non-commercial antibody T6 (page1, from S. Kloehn et al.). The T6 antibody is described in: Ouhtit A, Ronsin B, Kelly PA, Morel G. Ultrastructural expression of prolactin receptor in rat liver. Biol Cell. 1994;82(2-3):169-76 Okamura H, Zachwieja J, Raguet S, Kelly PA. Characterization and applications of monoclonal antibodies to the prolactin receptor. Endocrinology. 1989 May;124(5):2499-508. So you can see that the results obtained with AB2773 are still not very good. If you have any more suggestions on how the results can be imroved they will be highly appreciated. (Please notice that I am on holiday next week, but my collegue Christian Erlenhoefer will take my place during that time) Previous message: I have a complaint about your Prolactin Receptor antibody #AB2773: Our customer used it in IHC staining on formalin-fixed, paraffin embedded tissue sections of rat liver and pituitary. Protocol: Antigen Retrieval: - with 10 mM Citrate buffer pH 6.0 using a Microwave Blocking: 5 min 0.5% Triton in TBS wash with TBS 20-25 min 0-35% H2O2 in TBS/Methanol (90/10) wash 3x with TBS 10 min DAKO blocking solution A 10 min DAKO blocking solution B wash 3x with TBS Primary antibody: AB2773 (Lot 19068) 1/50 in antibody diluent (DAKO) for 1h at RT wash 3x with TBS Secondary antibody: biotinylated secondary antibody from DAKO (LSAB-Kit, ready to use) for 30 min at RT wash 3x with TBS Streptavidin-Biotin-Complex (DAKO) for 30 min at RT wash 3x with TBS Detection with AEC-Substrate Kit. Counterstaining in Hämalaun, embedding in Kaisers Glycine-Gelatine. Problem: no specific staining could be obtained. The same protocol was used successfully using a Ki67 antibody on rat tonsil. Please hava a look at the attached pictures.

    Read More

    Abcam community

    Verified customer

    Asked on Jul 12 2004

    Answer

    Here is a reference that uses our monoclonal antibodies with IHC procedures: Rl Sorenson and LE Stout. Prolactin Receptors and JAK2 in Islets of Langerhans: An Immunohistochemical Analysis. Endocrinology 136:9 pp 4092-4098. 1995 Also used in FACS analysis: M. Gagnerault, et al. Expression of Prolactin Receptors in Murine Lymphoid Cells in Normal and Autoimmune Situations. J. Immunol., 150(12): 5673-5681, 1993. The customer should follow these protocols along with the other information have passed onto him/her with our previous correspondence. If not, please let us know how you would like to proceed. This will work, otherwise, there is another issue and not the antibody. Please let us know if this helps or if you require further assistance. Thank you,

    Read More

    Abcam Scientific Support

    回复于 Jul 14 2004

    Question

    do you know if the antibody AB2773 to Prolactin Receptor is specific to the long or short form of the receptor? Do you have an antibody available, that is specific for the long form only?

    Read More

    Abcam community

    Verified customer

    Asked on May 03 2004

    Answer

    Ab2773 has been shown to be specific for the short form around 42 kDa. The other Prolactin Receptor antibody that we currently have, ab2772, detects both the short and long forms. So unfortunately we don't have what your customer is looking for.

    Read More

    Abcam Scientific Support

    回复于 May 06 2004

    Question

    I would like to purchase a trial size (enough for 1 immunoblot) i.e. 20 ug of both the U5 (ab2772) and T6 (ab2773)- prolactin receptor antibodies to try out. Is this possible and can you give me a quote please.

    Read More

    Abcam community

    Verified customer

    Asked on Oct 13 2003

    Answer

    We do not routinely offer free or trial sized samples for testing purposes. Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we will offer a replacement or reimbursement. Should you decide to test an antibody in an application for which we do not have any information, please let us know how you get on and in return we will forward a GBP10/ USD15/ EUR15 Amazon gift voucher.

    Read More

    Asdf Edo

    Abcam Scientific Support

    回复于 Oct 13 2003

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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