AB183968

重组Anti-Prolactin/PRL抗体[EPR19372]

Anti-Prolactin/PRL antibody [EPR19372]

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal Prolactin/PRL antibody. Suitable for IHC-Fr, IP, WB and reacts with Mouse, Rat samples. Cited in 1 publication.

查看别名

Prolactin, PRL, Prl

4 Images

Immunohistochemistry (Frozen sections) - Anti-Prolactin/PRL antibody [EPR19372] (AB183968)

IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Prolactin/PRL antibody [EPR19372] (AB183968)

Immunohistochemical analysis of 4% paraformaldehyde-fixed 0.2% Triton X-100 permeabilized frozen Mouse pituitary tissue labeling Prolactin/PRL with ab183968 at 1/200 dilution followed by Goat Anti-Rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

The result showed cytoplasmic staining on mouse anterior pituitary.

The nuclear counterstain is DAPI (blue).

Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is ab150077 at 1/1000 dilution.

IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Prolactin/PRL antibody [EPR19372] (AB183968)

Immunohistochemical analysis of 4% paraformaldehyde-fixed 0.2% Triton X-100 permeabilized frozen Mouse kidney tissue labeling Prolactin/PRL with ab183968 at 1/200 dilution followed by Goat Anti-Rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

The result showed negative staining on mouse kidney.

The nuclear counterstain is DAPI (blue).

Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is ab150077 at 1/1000 dilution.

Supplier Data

Immunoprecipitation - Anti-Prolactin/PRL antibody [EPR19372] (AB183968)

Prolactin/PRL was immunoprecipitated from 0.35 mg of mouse pituitary whole cell lysate with ab183968 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab183968 at 1/5000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1 : Mouse pituitary whole cell lysate, 10μg (Input).

Lane 2 : ab183968 IP in Mouse pituitary whole cell lysate.

Lane 3 : Rabbit IgG,monoclonal [EPR25A]-Isotype Control (ab172730) instead of ab183968 in Mouse pituitary whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 10 seconds.

All lanes:

Immunoprecipitation - Anti-Prolactin/PRL antibody [EPR19372] (ab183968)

Predicted band size: 26 kDa

false

Supplier Data

Western blot - Anti-Prolactin/PRL antibody [EPR19372] (AB183968)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time : Lane 1 : 8 seconds; Lane 2,3,4,5 and 6 : 3 second.

All lanes:

Western blot - Anti-Prolactin/PRL antibody [EPR19372] (ab183968) at 1/5000 dilution

Lane 1:

Mouse pituitary lysate at 20 µg

Lane 2:

Rat pituitary lysate at 20 µg

Lane 3:

Mouse uterus tissue lysate at 20 µg

Lane 4:

Rat kidney tissue lysate at 20 µg

Lane 5:

C6 (Rat glial tumor cell line) whole cell lysate at 20 µg

Lane 6:

NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 26 kDa

Observed band size: 25 kDa

false

不同偶联物与剂型 (1)

Carrier free

Anti-Prolactin/PRL antibody [EPR19372] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR19372

亚型

IgG

不含载体蛋白

反应种属

Mouse, Rat

应用

IHC-Fr, IP, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/200", "IHCFr-species-notes": "Antigen retrieval: Heated citrate solution (10mM citrate pH 6.0 + 0.05% Tween-20).This application is recommended for mouse only.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "" }, "Rat": { "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "" } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Prolactin (PRL) also known as mammotropin is a peptide hormone with a molecular mass of approximately 23 kDa. PRL is primarily expressed in the anterior pituitary gland but can also be found in other tissues such as the mammary glands brain and immune system. PRL plays an important role in lactation stimulating milk production in mammals. The PRL protein functions by binding to the prolactin receptor a member of the cytokine receptor family initiating signal transduction pathways.

Biological function summary

PRL influences a variety of physiological processes including reproduction metabolism and osmoregulation. It acts as a single entity rather than part of a larger protein complex affecting development and growth through its regulatory activities. In addition to lactation PRL also plays roles in the immune system cell proliferation and the production of surfactant in the lungs.

Pathways

Prolactin is deeply involved in the JAK-STAT signaling pathway and the MAPK/ERK pathway which are pivotal for transmitting signals from the prolactin receptor to the nucleus. Through these pathways PRL interacts with proteins like Janus kinase (JAK) and Signal Transducer and Activator of Transcription (STAT) proteins facilitating its wide range of biological effects. This interplay helps regulate gene expression critical for various physiological conditions.

PRL is linked to conditions such as hyperprolactinemia and prolactinoma. Hyperprolactinemia characterized by elevated levels of prolactin in the blood can lead to symptoms such as galactorrhea and infertility. In the context of prolactinoma an adenoma of the pituitary gland PRL's overproduction is common. These disorders often relate to proteins like dopamine which typically acts to inhibit PRL secretion from the pituitary gland and any disruption in this regulation can result in the aforementioned conditions.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Prolactin acts primarily on the mammary gland by promoting lactation.

See full target information Prl

其他靶点

Prolactin/PRL

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Neuroendocrinology 114:517-537 PubMed38071956

2023

Oxytocin Receptor-Expressing Neurons in the Medial Preoptic Area Are Essential for Lactation, whereas Those in the Lateral Septum Are Not Critical for Maternal Behavior.

Applications

Unspecified application

Species

Unspecified reactive species

Shizu Hidema,Keisuke Sato,Hiroaki Mizukami,Yumi Takahashi,Yuko Maejima,Kenju Shimomura,Katsuhiko Nishimori

View all publications

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Abcam Product Promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

重组Anti-Prolactin/PRL抗体[EPR19372]