AB188229

重组Anti-Prolactin/PRL抗体[EPR18018-31]

Anti-Prolactin/PRL antibody [EPR18018-31]

RabMAb
Recombinant
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(9 Publications)

Rabbit Recombinant Monoclonal Prolactin/PRL antibody. Suitable for IHC-Fr, WB, IHC-P, IP and reacts with Mouse, Rat samples. Cited in 9 publications.

查看别名

Prolactin, PRL, Prl

5 Images

Immunohistochemistry (Frozen sections) - Anti-Prolactin/PRL antibody [EPR18018-31] (AB188229)

IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Prolactin/PRL antibody [EPR18018-31] (AB188229)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized mouse pituitary tissue labeling Prolactin/PRL with ab188229 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution (green). Cytoplasmic staining in lactotrophic cells of anterior pituitary gland (PMID : 16468032). The nuclear counter stain is DAPI (blue).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prolactin/PRL antibody [EPR18018-31] (AB188229)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prolactin/PRL antibody [EPR18018-31] (AB188229)

Immunohistochemical analysis of paraffin-embedded mouse pituitary tissue labeling Prolactin/PRL with ab188229 at 1/16000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on mouse pituitary (PMID : 24514953). Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prolactin/PRL antibody [EPR18018-31] (AB188229)

Immunohistochemical analysis of paraffin-embedded rat pituitary tissue labeling Prolactin/PRL with ab188229 at 1/16000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on rat pituitary (PMID : 24514953). Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Supplier Data

Immunoprecipitation - Anti-Prolactin/PRL antibody [EPR18018-31] (AB188229)

Prolactin/PRL was immunoprecipitated from 0.35 mg of mouse pituitary tissue lysate with ab188229 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab188229 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1 : Mouse pituitary tissue lysate 10 μg (Input).

Lane 2 : ab188229 IP in mouse pituitary tissue lysate.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab188229 in mouse pituitary tissue lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 1 second.

All lanes:

Immunoprecipitation - Anti-Prolactin/PRL antibody [EPR18018-31] (ab188229)

Predicted band size: 26 kDa

Observed band size: 23 kDa

false

Supplier Data

Western blot - Anti-Prolactin/PRL antibody [EPR18018-31] (AB188229)

Blocking/Dilution buffer : 5% NFDM/TBST

Prolactin expression is generally restricted to the anterior pituitary gland (PMID : 1697858).

Negative control : Mouse kidney, rat heart and C6 cells.

All lanes:

Western blot - Anti-Prolactin/PRL antibody [EPR18018-31] (ab188229) at 1/5000 dilution

Lane 1:

Rat pituitary tissue lysate at 20 µg

Lane 2:

Mouse pituitary tissue lysate at 20 µg

Lane 3:

Mouse kidney tissue lysate at 20 µg

Lane 4:

Rat heart tissue lysate at 20 µg

Lane 5:

C6 (rat glial tumor cell line) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 26 kDa

Observed band size: 23 kDa

true

Exposure time: 5s

不同偶联物与剂型 (2)

Carrier free

Anti-Prolactin/PRL antibody [EPR18018-31] - BSA and Azide free
Carrier free

Anti-Prolactin/PRL antibody [EPR18018-31] - BSA and Azide free (Capture)

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR18018-31

亚型

IgG

不含载体蛋白

反应种属

Mouse, Rat

应用

IP, IHC-P, IHC-Fr, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/1000", "IHCFr-species-notes": "Perform heat mediated antigen retrieval with 10 mM sodium citrate buffer pH 6.0 + 0.05% Tween-20 before commencing with IHC staining protocol.Under our IHC-Fr experimental conditions this antibody could not detect Prolactin on rat pituitary gland sample.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/16000", "IHCP-species-notes": "This antibody is not suitable for human species in IHC application.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "" }, "Rat": { "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/16000", "IHCP-species-notes": "This antibody is not suitable for human species in IHC application.", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "" } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Prolactin (PRL) also known as mammotropin is a peptide hormone with a molecular mass of approximately 23 kDa. PRL is primarily expressed in the anterior pituitary gland but can also be found in other tissues such as the mammary glands brain and immune system. PRL plays an important role in lactation stimulating milk production in mammals. The PRL protein functions by binding to the prolactin receptor a member of the cytokine receptor family initiating signal transduction pathways.

Biological function summary

PRL influences a variety of physiological processes including reproduction metabolism and osmoregulation. It acts as a single entity rather than part of a larger protein complex affecting development and growth through its regulatory activities. In addition to lactation PRL also plays roles in the immune system cell proliferation and the production of surfactant in the lungs.

Pathways

Prolactin is deeply involved in the JAK-STAT signaling pathway and the MAPK/ERK pathway which are pivotal for transmitting signals from the prolactin receptor to the nucleus. Through these pathways PRL interacts with proteins like Janus kinase (JAK) and Signal Transducer and Activator of Transcription (STAT) proteins facilitating its wide range of biological effects. This interplay helps regulate gene expression critical for various physiological conditions.

PRL is linked to conditions such as hyperprolactinemia and prolactinoma. Hyperprolactinemia characterized by elevated levels of prolactin in the blood can lead to symptoms such as galactorrhea and infertility. In the context of prolactinoma an adenoma of the pituitary gland PRL's overproduction is common. These disorders often relate to proteins like dopamine which typically acts to inhibit PRL secretion from the pituitary gland and any disruption in this regulation can result in the aforementioned conditions.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Prolactin acts primarily on the mammary gland by promoting lactation.

See full target information Prl

其他靶点

Prolactin/PRL

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文献 (9)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 16:710 PubMed41057318

2025

CTRP9 as a myokine mitigates sarcopenia via the LAMP-2A/NLRP3 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Linxi Li,Anju Zuo,Ruoyu Yin,Qiangqiang Liu,Chen Liu,Na Li,Dan Xu,Shaomeng Zhang,Jiarui Li,Shengyun Lei,Shiyan Ruan,Tingting Li,Yuan Guo

Journal of neuroinflammation 21:295 PubMed39543619

2024

Prolactin deficiency drives diabetes-associated cognitive dysfunction by inducing microglia-mediated synaptic loss.

Applications

Unspecified application

Species

Unspecified reactive species

Jiaxuan Jiang,Pengzi Zhang,Yue Yuan,Xiang Xu,Tianyu Wu,Zhou Zhang,Jin Wang,Yan Bi

Clinical and translational medicine 14:e1564 PubMed38344897

2024

The involvement of RNA N6-methyladenosine and histone methylation modification in decidualization and endometriosis-associated infertility.

Applications

Unspecified application

Species

Unspecified reactive species

Xiang Lin,Yongdong Dai,Weijia Gu,Yi Zhang,Feng Zhuo,Fanxuan Zhao,Xiaoying Jin,Chao Li,Dong Huang,Xiaomei Tong,Songying Zhang

Molecular medicine (Cambridge, Mass.) 28:100 PubMed36050637

2022

Temporal changes in cyclinD-CDK4/CDK6 and cyclinE-CDK2 pathways: implications for the mechanism of deficient decidualization in an immune-based mouse model of unexplained recurrent spontaneous abortion.

Applications

Unspecified application

Species

Unspecified reactive species

Zhuo Chang,Hai-Xue Kuang,Xueming Zhou,Hui Zhu,Yang Zhang,Yin Fu,Qiang Fu,Bei Jiang,Wei Wang,Sha Jiang,Li Ren,Lei Ma,Xue Pan,Xiao-Ling Feng

The Journal of pathology 258:49-57 PubMed35657600

2022

Low-rank fusion convolutional neural network for prediction of remission after stereotactic radiosurgery in patients with acromegaly: a proof-of-concept study.

Applications

Unspecified application

Species

Unspecified reactive species

Nidan Qiao,Damin Yu,Guoqing Wu,Qilin Zhang,Boyuan Yao,Min He,Hongying Ye,Zhaoyun Zhang,Yongfei Wang,Hanfeng Wu,Yao Zhao,Jinhua Yu

Reproductive biology and endocrinology : RB&E 20:76 PubMed35501804

2022

Increased expression of prokineticin 2 and its receptor in endometrium of recurrent implantation failure patients decreased the expression of MMP9 important for decidualization.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Zhai,Linna Ma,Ziyin Chang,Ting Yu

Journal of assisted reproduction and genetics 38:2641-2650 PubMed34718923

2021

TOB1 modulates the decidualization of human endometrial stromal cells via the Notch pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yaling Jiang,Xinhua Yuan,Bing Li,Mingxing Liu,Yu Shi,Jianhuai Feng,Hua Zhou,Miaoxian Ou,Xiaozhen Huang

Journal of advanced research 30:15-25 PubMed34026283

2021

Decreased PIBF1/IL6/p-STAT3 during the mid-secretory phase inhibits human endometrial stromal cell proliferation and decidualization.

Applications

Unspecified application

Species

Unspecified reactive species

Mingjuan Zhou,Huihui Xu,Dan Zhang,Chenchen Si,Xiaowei Zhou,Hui Zhao,Qiang Liu,Bufang Xu,Aijun Zhang

Aging 13:1276-1293 PubMed33472173

2020

Genomic and transcriptomic analysis of pituitary adenomas reveals the impacts of copy number variations on gene expression and clinical prognosis among prolactin-secreting subtype.

Applications

Unspecified application

Species

Unspecified reactive species

Yiyuan Chen,Hua Gao,Weiyan Xie,Jing Guo,Qiuyue Fang,Peng Zhao,Chunhui Liu,Haibo Zhu,Zhuang Wang,Jichao Wang,Songbai Gui,Yazhuo Zhang,Chuzhong Li

View all publications

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重组Anti-Prolactin/PRL抗体[EPR18018-31]