Anti-Prohibitin抗体[PHB/1882] (ab224657)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prohibitin antibody [PHB/1882] (ab224657)](https://www.abcam.cn/ps/products/224/ab224657/Images/ab224657-287622-anti-prohibitin-antibody-phb-1882-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prohibitin antibody [PHB/1882] (ab224657)")
Key features and details
- Mouse monoclonal [PHB/1882] to Prohibitin
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG1
概述
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产品名称
Anti-Prohibitin抗体[PHB/1882]
参阅全部 Prohibitin 一抗 -
描述
小鼠单克隆抗体[PHB/1882] to Prohibitin -
宿主
Mouse -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment within Human Prohibitin aa 167-261. The exact sequence is proprietary.
Database link: P35232 -
阳性对照
- IHC-P: Human liver tissue; WB: Ramos, HEK-293, HepG2, Jurkat, A549, K562, A431, and MCF7 cell lysates. Human liver tissue lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.05% BSA, PBS -
Concentration information loading... -
纯度
Protein A/G purified -
纯化说明
Purified from bioreactor concentrate. -
克隆
单克隆 -
克隆编号
PHB/1882 -
同种型
IgG1 -
轻链类型
kappa -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab224657于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 30 kDa.
|
|
| IHC-P |
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
| 说明 |
|---|
|
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 30 kDa. |
|
IHC-P
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
靶标
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功能
Prohibitin inhibits DNA synthesis. It has a role in regulating proliferation. As yet it is unclear if the protein or the mRNA exhibits this effect. May play a role in regulating mitochondrial respiration activity and in aging. -
组织特异性
Widely expressed in different tissues. -
序列相似性
Belongs to the prohibitin family. -
发展阶段
Levels of expression in fibroblasts decrease heterogeneously during cellular aging. -
细胞定位
Mitochondrion inner membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 5245 Human
- Omim: 176705 Human
- SwissProt: P35232 Human
- Unigene: 514303 Human
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别名
- Epididymis luminal protein 215 antibody
- Epididymis secretory sperm binding protein Li 54e antibody
- HEL 215 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prohibitin antibody [PHB/1882] (ab224657)
Formalin-fixed, paraffin-embedded human liver tissue stained for Prohibitin with ab224657 at 2 µg/ml in immunohistochemical analysis.
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![Western blot - Anti-Prohibitin antibody [PHB/1882] (ab224657)](https://www.abcam.cn/ps/products/224/ab224657/Images/ab224657-455291-anti-prohibitin-antibody-phb-1882-western-blot-ramos-hek293-k562-jurkat-hepg2-human.jpg)
Western blot - Anti-Prohibitin antibody [PHB/1882] (ab224657)All lanes : Anti-Prohibitin antibody [PHB/1882] (ab224657) at 2 µg/ml
Lane 1 : Ramos (human Burkitt's lymphoma cell line) whole cell lysate
Lane 2 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate
Lane 3 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
Lane 4 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 5 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Predicted band size: 30 kDa -
![Western blot - Anti-Prohibitin antibody [PHB/1882] (ab224657)](https://www.abcam.cn/ps/products/224/ab224657/Images/ab224657-455290-anti-prohibitin-antibody-phb-1882-western-blot-liver-human.jpg)
Western blot - Anti-Prohibitin antibody [PHB/1882] (ab224657)Anti-Prohibitin antibody [PHB/1882] (ab224657) at 2 µg/ml + Human liver tissue lysate
Predicted band size: 30 kDa -
![Western blot - Anti-Prohibitin antibody [PHB/1882] (ab224657)](https://www.abcam.cn/ps/products/224/ab224657/Images/ab224657-287623-anti-prohibitin-antibody-phb-1882-western-blot.jpg)
Western blot - Anti-Prohibitin antibody [PHB/1882] (ab224657)All lanes : Anti-Prohibitin antibody [PHB/1882] (ab224657) at 1 µg/ml
Lane 1 : A549 (human lung carcinoma cell line) cell lysate
Lane 2 : K562 (human chronic myelogenous leukemia cell line from bone marrow ) cell lysate
Lane 3 : A431 (human epidermoid carcinoma cell line) cell lysate
Lane 4 : MCF7 (human breast adenocarcinoma cell line) cell lysate
Developed using the ECL technique.
Predicted band size: 30 kDa
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab224657 尚未被引用在任何文献中。