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重组RabMAb

重组Anti-Progesterone Receptor抗体[SP2] (ab16661)

Reviews (4)Q&A (5)References (40)
Multiplex immunohistochemistry - Anti-Progesterone Receptor antibody [SP2] (ab16661)
  • Immunocytochemistry/ Immunofluorescence - Anti-Progesterone Receptor antibody [SP2] (ab16661)
  • Flow Cytometry - Anti-Progesterone Receptor antibody [SP2] (ab16661)
  • Multiplex immunohistochemistry - Anti-Progesterone Receptor antibody [SP2] (ab16661)
  • Multiplex immunohistochemistry - Anti-Progesterone Receptor antibody [SP2] (ab16661)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Progesterone Receptor antibody [SP2] (ab16661)
  • Flow Cytometry - Anti-Progesterone Receptor antibody [SP2] (ab16661)
  • Immunocytochemistry/ Immunofluorescence - Anti-Progesterone Receptor antibody [SP2] (ab16661)
  • Anti-Progesterone Receptor antibody [SP2] (ab16661)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [SP2] to Progesterone Receptor
  • Suitable for: ICC/IF, IHC-P, mIHC, Flow Cyt
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647 Carrier Free

概述

  • 产品名称

    Anti-Progesterone Receptor抗体[SP2]
    参阅全部 Progesterone Receptor 一抗

  • 描述

    兔单克隆抗体[SP2] to Progesterone Receptor

  • 宿主

    Rabbit

  • 经测试应用

    适用于: ICC/IF, IHC-P, mIHC, Flow Cytmore details

  • 种属反应性

    与反应: Human
    预测可用于: Rat, Rabbit

  • 免疫原

    Recombinant fragment. This information is considered to be commercially sensitive.

  • 阳性对照

    • Breast carcinomas IHC-P: Human breast carcinoma tissue. ICC/IF: T-47D cells Flow Cyt: T-47D cells mIHC: Human mammary gland tissue sections, Human triple-positive breast carcinoma tissue sections

  • 常规说明

    This product was switched from a hybridoma to recombinant production method on 13th November 2019.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab16661于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC/IF
1/100.
IHC-P (3)
1/400.

Staining of formalin-fixed tissues is required by boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
mIHC
1/6000.
Flow Cyt
1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
说明
ICC/IF
1/100.
IHC-P
1/400.

Staining of formalin-fixed tissues is required by boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
mIHC
1/6000.
Flow Cyt
1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

靶标

  • 功能

    The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Progesterone receptor isoform B (PRB) is involved activation of c-SRC/MAPK signaling on hormone stimulation.
    Isoform A: inactive in stimulating c-Src/MAPK signaling on hormone stimulation.
    Isoform 4: Increases mitochondrial membrane potential and cellular respiration upon stimulation by progesterone.

  • 序列相似性

    Belongs to the nuclear hormone receptor family. NR3 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.

  • 结构域

    Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.

  • 翻译后修饰

    Phosphorylated on multiple serine sites. Several of these sites are hormone-dependent. Phosphorylation on Ser-294 occurs preferentially on isoform B, is highly hormone-dependent and modulates ubiquitination and sumoylation on Lys-388. Phosphorylation on Ser-102 and Ser-345 also requires induction by hormone. Basal phosphorylation on Ser-81, Ser-162, Ser-190 and Ser-400 is increased in response to progesterone and can be phosphorylated in vitro by the CDK2-A1 complex. Increased levels of phosphorylation on Ser-400 also in the presence of EGF, heregulin, IGF, PMA and FBS. Phosphorylation at this site by CDK2 is ligand-independent, and increases nuclear translocation and transcriptional activity. Phosphorylation at Ser-162 and Ser-294, but not at Ser-190, is impaired during the G(2)/M phase of the cell cycle. Phosphorylation on Ser-345 by ERK1/2 MAPK is required for interaction with SP1.
    Sumoylation is hormone-dependent and represses transcriptional activity. Sumoylation on all three sites is enhanced by PIAS3. Desumoylated by SENP1. Sumoylation on Lys-388, the main site of sumoylation, is repressed by ubiquitination on the same site, and modulated by phosphorylation at Ser-294.
    Ubiquitination is hormone-dependent and represses sumoylation on the same site. Promoted by MAPK-mediated phosphorylation on Ser-294.
    Palmitoylated by ZDHHC7 and ZDHHC21. Palmitoylation is required for plasma membrane targeting and for rapid intracellular signaling via ERK and AKT kinases and cAMP generation.

  • 细胞定位

    Nucleus. Cytoplasm. Nucleoplasmic shuttling is both homone- and cell cycle-dependent. On hormone stimulation, retained in the cytoplasm in the G(1) and G(2)/M phases; Mitochondrion outer membrane and Nucleus. Cytoplasm. Mainly nuclear.
  • Information by UniProt

  • 数据库链接

    see all

  • 别名

    • NR3C3 antibody
    • Nuclear receptor subfamily 3 group C member 3 antibody
    • PGR antibody
    • PR antibody
    • PRA antibody
    • PRB antibody
    • PRGR_HUMAN antibody
    • Progesterone receptor antibody
    • Progestin receptor form A antibody
    • Progestin receptor form B antibody
    see all

图片

  • Multiplex immunohistochemistry - Anti-Progesterone Receptor antibody [SP2] (ab16661)
    Multiplex immunohistochemistry - Anti-Progesterone Receptor antibody [SP2] (ab16661)

    Multiplex immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human triple-positive breast carcinoma tissue sections labeling Progesterone Receptor (PR) with ab16661, at a 1/6000 dilution ( 0.2 μg/ml). Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins and Opal Polymer HRP Ms + Rb was used as the secondary antibody. DAPI was used as the nuclear counterstain.

    Panel A: merged staining of anti-Progesterone Receptor (PR) (magenta; Opal™690), anti-HER2 (red; Opal™570) and anti-Estrogen Receptor (ER) (green; Opal™520) on human triple-positive breast carcinoma.
    Panel B: anti-PR stained on nucleus of cancer cells.
    Panel C: anti-HER2 stained on membrane of cancer cells.
    Panel D: anti-ER stained on nucleus of cancer cells.

    The section was incubated in three rounds of staining: in the order of ab16661 for 30 mins, then ab16660 and ab231438 for 10 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

  • Immunocytochemistry/ Immunofluorescence - Anti-Progesterone Receptor antibody [SP2] (ab16661)
    Immunocytochemistry/ Immunofluorescence - Anti-Progesterone Receptor antibody [SP2] (ab16661)

    Immunocytochemistry/ Immunofluorescence analysis of T-47D (human ductal breast epithelial tumor epithelial cell) cells labeling Progesterone Receptor with purified ab16661 at 1/100 (2.28 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    This image was generated from the hybridoma version.

     

  • Flow Cytometry - Anti-Progesterone Receptor antibody [SP2] (ab16661)
    Flow Cytometry - Anti-Progesterone Receptor antibody [SP2] (ab16661)

    Flow Cytometry analysis of T-47D (human ductal breast epithelial tumor epithelial cell) cells labeling Progesterone Receptor with purified ab16661 at 1/220 dilution (1.04 µg/ml) - Red. Cells were fixed with 4% paraformaldehyde . A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) - Black. Unlabeled control - Blue.

    This image was generated from the hybridoma version.

     

  • Multiplex immunohistochemistry - Anti-Progesterone Receptor antibody [SP2] (ab16661)
    Multiplex immunohistochemistry - Anti-Progesterone Receptor antibody [SP2] (ab16661)

    Multiplex immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human mammary gland tissue sections labeling Progesterone Receptor (PR) with ab16661, at a 1/6000 dilution ( 0.2 μg/ml). Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins and Opal Polymer HRP Ms + Rb was used as the secondary antibody. DAPI was used as the nuclear counterstain.

    Panel A: merged staining of anti-Progesterone Receptor (PR) (magenta; Opal™690), anti-HER2 (red; Opal™570) and anti-Estrogen Receptor (ER) (green; Opal™520) on human mammary gland.
    Panel B: anti-PR stained on nucleus of some ductal cells.
    Panel C: anti-HER2 stained on no cells.
    Panel D: anti-ER stained on nucleus of some ductal cells.

    The section was incubated in three rounds of staining: in the order of ab16661 for 30 mins, then ab16660 and ab231438 for 10 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

  • Multiplex immunohistochemistry - Anti-Progesterone Receptor antibody [SP2] (ab16661)
    Multiplex immunohistochemistry - Anti-Progesterone Receptor antibody [SP2] (ab16661)

    Multiplex immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human triple-negative breast carcinoma tissue sections labeling Progesterone Receptor (PR) with ab16661, at a 1/6000 dilution ( 0.2 μg/ml). Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins and Opal Polymer HRP Ms + Rb was used as the secondary antibody. DAPI was used as the nuclear counterstain.

    Panel A: merged staining of anti-Progesterone Receptor (PR) (magenta; Opal™690), anti-HER2 (red; Opal™570) and anti-Estrogen Receptor (ER) (green; Opal™520) on human triple-negative breast carcinoma.
    Panel B: anti-PR stained on no cells.
    Panel C: anti-HER2 stained on no cells.
    Panel D: anti-ER stained on no cells.

    The section was incubated in three rounds of staining: in the order of ab16661 for 30 mins, then ab16660 and ab231438 for 10 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Progesterone Receptor antibody [SP2] (ab16661)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Progesterone Receptor antibody [SP2] (ab16661)

    Immunohistochemistry analysis of human breast carcinoma tissue labelling SP2 with ab16661.

    This image was generated from the hybridoma version.

     

  • Flow Cytometry - Anti-Progesterone Receptor antibody [SP2] (ab16661)
    Flow Cytometry - Anti-Progesterone Receptor antibody [SP2] (ab16661)

    Overlay histogram showing T47D cells stained with ab16661 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab16661, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    This image was generated from the hybridoma version.

     

  • Immunocytochemistry/ Immunofluorescence - Anti-Progesterone Receptor antibody [SP2] (ab16661)
    Immunocytochemistry/ Immunofluorescence - Anti-Progesterone Receptor antibody [SP2] (ab16661)Ding L, et al. (2019), PLOS Genetics, 15(3), e1008002. Fig 3D, DOI: 10.1371/journal.pgen.1008002. Reproduced under the Creative Commons licence. https://creativecommons.org/licenses/by/4.0/

    Rat mammary epithelial cells stained for Pr (pink) using ab16661 at 1/100 dilution in ICC/IF. 

  • Anti-Progesterone Receptor antibody [SP2] (ab16661)
    Anti-Progesterone Receptor antibody [SP2] (ab16661)

文献 (40)

发表研究结果有使用 ab16661？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab16661 被引用在 40 文献中.

  • Alradhi M  et al. Molecular genetic and clinical characteristic analysis of primary signet ring cell carcinoma of urinary bladder identified by a novel OR2L5 mutation. Cancer Med 12:3931-3951 (2023). PubMed: 36779496
  • Shen L  et al. Integrated transcriptomics, proteomics, and functional analysis to characterize the tissue-specific small extracellular vesicle network of breast cancer. MedComm (2020) 4:e433 (2023). PubMed: 38053815
  • Lee JH & Kim SH Functional and morphological maturation of the full-sized and mini-pig corpus luteum by programmed cell death mechanism. J Vet Res 67:307-314 (2023). PubMed: 38143820
  • Xie Y  et al. A delayed ovulation of progestin-primed ovarian stimulation (PPOS) by downregulating the LHCGR/PGR pathway. iScience 26:107357 (2023). PubMed: 37520702
  • Huang J  et al. Human amniotic mesenchymal stem cells combined with PPCNg facilitate injured endometrial regeneration. Stem Cell Res Ther 13:17 (2022). PubMed: 35022063
View all Publications for this product

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1-9 of 9 Abreviews or Q&A

Western blot abreview for Anti-Progesterone Receptor antibody [SP2]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (U937 cells)
Gel Running Conditions
Reduced Denaturing (4-12% Tris-Bis gel)
Loading amount
40 µg
Specification
U937 cells
Blocking step
Milk as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Mar 10 2022

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Progesterone Receptor antibody [SP2]

 Excellent
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (breast cancer)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: BOND ER2
Permeabilization
No
Specification
breast cancer
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Dec 13 2021

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Progesterone Receptor antibody [SP2]

 Good
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 15 minute(s) · Concentration: 20% · Temperature: RT°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: pH9
Sample
Human Tissue sections (Breast carcinoma)
Specification
Breast carcinoma
Permeabilization
No
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Jul 15 2013

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Progesterone Receptor antibody [SP2]

 Average
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Rat ovary, uterus)
Specification
Rat ovary, uterus
Fixative
10% buffered formalin
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM citrate buffer pH 6.0
Permeabilization
No
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 20°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Nov 27 2007

Question

I recently purchased an antibody from yourselves – Progesterone receptor, ab16661, lot: GR52327-3.
Please would you be able to tell me the protein concentration of this antibody.
Many Thanks

Read More
Answer

Thank you for your enquiry.
I can confirm that ab16661 antibody is sold as tissue culture supernatant. Unpurified antibodies, such as those sold as whole antiserum, ascites or tissue culture supernatant will not have a concentration stated on the datasheet. Antibody concentration is usually determined by protein assay, and serum / ascites / tissue culture supernatant will contain a lot of other proteins, which means the antibody quantification would not be accurate.
I can confirm that for tissue culture supernatant, concentration of antibody is known to very between 1 - 3 mg/ml
I am sorry we are not able to provide an exact concentration on this occasion, but hope this information will be helpful to you. If you have any further questions, please do not hesitate to contact us.

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Question

Hi,

I recently purchased an antibody from yourselves – Progesterone receptor, ab16661, lot: GR52327-3.

Please would you be able to tell me the protein concentration of this antibody.

Many Thanks

Read More
Answer

Thank you for your enquiry.

I can confirm that ab16661 Progesterone Receptor antibody SP2 is sold as tissue culture supernatant. TC supernatant will not have a concentration stated on the datasheet. Antibody concentration is usually determined by protein assay, and tissue culture supernatant will contain a lot of other proteins, which means the antibody quantification would not be accurate.

I can confirm that for tissue culture supernatant, concentration of antibody is known to very between 1 - 3 mg/ml

I am sorry we are not able to provide an exact concentration on this occasion, but hope this information will be helpful to you. If you have any further questions, please do not hesitate to contact us.

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Question

Dear Sir/Madam Please kindly recommend the abcam slide control for IHC-P application for this antibodies. 1. Anti-ErbB 2 antibody (ab2428) 2. Anti-Progesterone Receptor antibody [SP2] (ab16661) 3. Anti-Estrogen Receptor antibody [SP1] (ab16660) 4. Anti-Myeloperoxidase antibody (ab45977) 5. Anti-TdT antibody (ab85148) 6. Anti-TMEM16A antibody (ab53212) 7. Anti-NSE antibody [EPR3377] (ab79757) 8. Anti-CD23 antibody [EPR3617(IHC)] (ab92495) 9. Anti-CD10 antibody [56C6] (ab951) 10. Anti-Melanoma antibody [HMB45 + DT101 + BC199] (ab732) 11. Anti-CD79a antibody [HM47/A9] (ab3121) 12. Anti-CD45RO antibody [UCHL-1] (ab86080) 13. Anti-CD45 antibody (ab10559) 14. Anti-CD30 antibody [CON6D/ C2] - BSA and Azide free (ab76518) 15. Anti-CD15 antibody [(Workshop IV) (BRA-4F1) our designation 238)] (ab115993) Best regards

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Answer

Thank you for your enquiry. I am pleased to provide the following suggested positive controls for these antibodies. I have mainly chosen tissue that has been tested succesfully and shown in an image on the datasheets: 1.Anti-ErbB 2 antibody (ab2428) Human breast cancer 2.Anti-Progesterone Receptor antibody [SP2] (ab16661) Human breast carcinoma 3.Anti-Estrogen Receptor antibody [SP1] (ab16660) Human breast carcinoma 4.Anti-Myeloperoxidase antibody (ab45977) Human spleen tissue 5.Anti-TdT antibody (ab85148) Human testis 6.Anti-TMEM16A antibody (ab53212) Human liver 7.Anti-NSE antibody [EPR3377] (ab79757) Human brain tissue 8.Anti-CD23 antibody [EPR3617(IHC)] (ab92495) Human tonsil 9.Anti-CD10 antibody [56C6] (ab951) Lymph node tissue or human breast microepithelium 10.Anti-Melanoma antibody [HMB45 + DT101 + BC199] (ab732) Murine melanoma metastasis tissue 11.Anti-CD79a antibody [HM47/A9] (ab3121) Human Lymphoid tissue, tonsil 12.Anti-CD45RO antibody [UCHL-1] (ab86080) Human Tonsil, Thymus 13.Anti-CD45 antibody (ab10559) Human Tonsil tissue 14.Anti-CD30 antibody [CON6D/ C2] - BSA and Azide free (ab76518) Human Hodgekins lymphoma tissue 15.Anti-CD15 antibody [(Workshop IV) (BRA-4F1) our designation 238)] (ab115993) Human Hodgekins lymphoma tissue Human spleen I am providing a list ofslides that we sell which I hope will be helpful to you. I can recommend to review the online datasheet for more details. Human brain: ab4305Brain (human): hippocampus normal tissue slides https://www.abcam.com/Brain-human-hippocampus-normal-tissue-slides-ab4305.html (or use the following: https://www.abcam.com/Brain-human-hippocampus-normal-tissue-slides-ab4305.html). Human lymph node: ab4350Lymph node (human) normal tissue slides IHC-P, ISH https://www.abcam.com/Lymph-node-human-normal-tissue-slides-ab4350.html (or use the following: https://www.abcam.com/Lymph-node-human-normal-tissue-slides-ab4350.html). Human testis: ab4373Testis (human) normal tissue slides IHC-P, ISH https://www.abcam.com/Testis-human-normal-tissue-slides-ab4373.html (or use the following: https://www.abcam.com/Testis-human-normal-tissue-slides-ab4373.html). Human liver: ab4348Liver (human) normal tissue slides IHC-P, ISH https://www.abcam.com/Liver-human-normal-tissue-slides-ab4348.html (or use the following: https://www.abcam.com/Liver-human-normal-tissue-slides-ab4348.html). Human spleen: ab4372Spleen (human) normal tissue slides IHC-P, ISH https://www.abcam.com/Spleen-human-normal-tissue-slides-ab4372.html (or use the following: https://www.abcam.com/Spleen-human-normal-tissue-slides-ab4372.html). I am sorry we do not have slides forthe following: Human tonsil Hodgekins lymphoma tissue Mouse melenoma tissue I hope this will be helpful to you. If you have any further questions,please do not hesitate to contact us.

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Question

 I want to konw if ab16661 (Rabbit monoclonal [SP2] to Progesterone Receptor) can be used for IHC of rat ovary tissue's Progesterone Receptor. I want a firm answer. Thank you! Best wishes!               

Read More
Answer

Thank you for contacting us. This antibody has been tested on human breast carcinoma tissue sections in our own lab and as you can see from image it produced best results. One of the customers has used this antibody on Rat ovary tissue; as you can see from Abreview this also produced good result based on the target localization however we do not know why customer has given three stars to the Abreview. It is actually a good image. I can confirm that the antibody can be used for staining of target on Rat sections. In case the product does not work after applying troubleshooting steps; full amount can be refunded. Please contact our distributor in China for full terms and conditions.    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information. Check the following compatible products: ab6012       Goat F(ab')2 polyclonal Secondary Antibody to Rabbit IgG - H&L (Biotin), pre-adsorbed ab6013       Goat F(ab')2 polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed ab6015      Goat F(ab')2 polyclonal Secondary Antibody to Rabbit IgG - H&L (AP), pre-adsorbed ab6021      Goat F(ab')2 polyclonal Secondary Antibody to Rabbit IgG - Fc (Biotin) ab6023      Goat F(ab')2 polyclonal Secondary Antibody to Rabbit IgG - Fc (HRP) ab6024      Goat F(ab')2 polyclonal Secondary Antibody to Rabbit IgG - Fc (AP) ab96895    Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (DyLight® 488), pre-adsorbed ab96896    Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (DyLight® 550), pre-adsorbed For complete please visit website at www.abcam.com

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Question

I want to konw if ab16661 (Rabbit monoclonal [SP2] to Progesterone Receptor) can be used for IHC of rat ovary tissue's Progesterone Receptor. I want a firm answer. Thank you!  

Read More
Answer

Thank you for contacting Abcam. Under our Abpromise we guarantee that ab16661 will work on rat tissue in IHC-P. As long as the protein is expressed in the ovarian tissues of the rat then this antibody will be able to detect it. If there is anything else I can help you with, please let me kn

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com