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Anti-Progesterone Receptor抗体[Alpha PR6] (ab2765)

Reviews (6)Q&A (7)References (34)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Progesterone Receptor antibody [Alpha PR6] (ab2765)
  • Immunocytochemistry/ Immunofluorescence - Anti-Progesterone Receptor antibody [Alpha PR6] - ChIP Grade (ab2765)
  • Western blot - Anti-Progesterone Receptor antibody [Alpha PR6] - ChIP Grade (ab2765)
  • Flow Cytometry - Anti-Progesterone Receptor antibody [Alpha PR6] - ChIP Grade (ab2765)

Key features and details

  • Mouse monoclonal [Alpha PR6] to Progesterone Receptor
  • Suitable for: IHC-P, WB, Flow Cyt, ICC/IF
  • Reacts with: Human
  • Isotype: IgG2a

概述

  • 产品名称

    Anti-Progesterone Receptor抗体[Alpha PR6]
    参阅全部 Progesterone Receptor 一抗

  • 描述

    小鼠单克隆抗体[Alpha PR6] to Progesterone Receptor

  • 宿主

    Mouse

  • 特异性

    Detects the B form of the progesterone receptor (PR). This antibody does not cross-react with estrogen receptor or glucocorticoid receptor.

  • 经测试应用

    适用于: IHC-P, WB, Flow Cyt, ICC/IFmore details

  • 种属反应性

    与反应: Human

  • 免疫原

    Full length protein corresponding to Chicken Progesterone Receptor. Progesterone receptor purified from chick oviduct cytosol.

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab2765于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-P (5)
Use a concentration of 5 µg/ml.
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 99 kDa.
Flow Cyt
Use 0.5µg for 106 cells.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
ICC/IF (1)
Use at an assay dependent concentration.
说明
IHC-P
Use a concentration of 5 µg/ml.
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 99 kDa.
Flow Cyt
Use 0.5µg for 106 cells.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
ICC/IF
Use at an assay dependent concentration.

靶标

  • 功能

    The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Progesterone receptor isoform B (PRB) is involved activation of c-SRC/MAPK signaling on hormone stimulation.
    Isoform A: inactive in stimulating c-Src/MAPK signaling on hormone stimulation.
    Isoform 4: Increases mitochondrial membrane potential and cellular respiration upon stimulation by progesterone.

  • 序列相似性

    Belongs to the nuclear hormone receptor family. NR3 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.

  • 结构域

    Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.

  • 翻译后修饰

    Phosphorylated on multiple serine sites. Several of these sites are hormone-dependent. Phosphorylation on Ser-294 occurs preferentially on isoform B, is highly hormone-dependent and modulates ubiquitination and sumoylation on Lys-388. Phosphorylation on Ser-102 and Ser-345 also requires induction by hormone. Basal phosphorylation on Ser-81, Ser-162, Ser-190 and Ser-400 is increased in response to progesterone and can be phosphorylated in vitro by the CDK2-A1 complex. Increased levels of phosphorylation on Ser-400 also in the presence of EGF, heregulin, IGF, PMA and FBS. Phosphorylation at this site by CDK2 is ligand-independent, and increases nuclear translocation and transcriptional activity. Phosphorylation at Ser-162 and Ser-294, but not at Ser-190, is impaired during the G(2)/M phase of the cell cycle. Phosphorylation on Ser-345 by ERK1/2 MAPK is required for interaction with SP1.
    Sumoylation is hormone-dependent and represses transcriptional activity. Sumoylation on all three sites is enhanced by PIAS3. Desumoylated by SENP1. Sumoylation on Lys-388, the main site of sumoylation, is repressed by ubiquitination on the same site, and modulated by phosphorylation at Ser-294.
    Ubiquitination is hormone-dependent and represses sumoylation on the same site. Promoted by MAPK-mediated phosphorylation on Ser-294.
    Palmitoylated by ZDHHC7 and ZDHHC21. Palmitoylation is required for plasma membrane targeting and for rapid intracellular signaling via ERK and AKT kinases and cAMP generation.

  • 细胞定位

    Nucleus. Cytoplasm. Nucleoplasmic shuttling is both homone- and cell cycle-dependent. On hormone stimulation, retained in the cytoplasm in the G(1) and G(2)/M phases; Mitochondrion outer membrane and Nucleus. Cytoplasm. Mainly nuclear.
  • Information by UniProt

  • 数据库链接

    • 别名

      • NR3C3 antibody
      • Nuclear receptor subfamily 3 group C member 3 antibody
      • PGR antibody
      • PR antibody
      • PRA antibody
      • PRB antibody
      • PRGR_HUMAN antibody
      • Progesterone receptor antibody
      • Progestin receptor form A antibody
      • Progestin receptor form B antibody
      see all

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Progesterone Receptor antibody [Alpha PR6] (ab2765)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Progesterone Receptor antibody [Alpha PR6] (ab2765)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) was performed on human uterus tissue. Antigen retrieval was performed using 10mM sodium citrate followed by microwave treatment for 8-15 minutes. Endogenous peroxidases were blocked in 3% H202-methanol for 15 minutes and tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab2765 (1:20) overnight in a humidified chamber. Tissues were washed in PBST and detection was performed using a secondary antibody conjugated to HRP. DAB staining buffer was applied and tissues were counterstained with hematoxylin and prepped for mounting. Images were taken at 40X magnification.

    • Immunocytochemistry/ Immunofluorescence - Anti-Progesterone Receptor antibody [Alpha PR6] - ChIP Grade (ab2765)
      Immunocytochemistry/ Immunofluorescence - Anti-Progesterone Receptor antibody [Alpha PR6] - ChIP Grade (ab2765)

      Immunocytochemistry/ Immunofluorescence analysis of T47D cells untreated (left) or stimulated with 100nm promegestone for 1 hour (right), labeling Progesterone Receptor with ab2765 (green). The cells were fixed with formalin for 15 minutes, permeabilized with 0.1% Triton X-100 in TBS for 10 minutes, and blocked with 3% Blocker BSA for 15 minutes at room temperature. Cells were stained with Anti-Progesterone Receptor antibody [Alpha PR6] - ChIP Grade (ab2765) at a dilution of 1/100 for 1 hour at 37C, and then incubated with a Alexa Fluor 488 goat anti-mouse IgG secondary antibody at a dilution of 1/1000 for 30 minutes at room temperature (both panels, green). Nuclei (both panels, blue) were stained with Hoechst 33342 dye.

    • Western blot - Anti-Progesterone Receptor antibody [Alpha PR6] - ChIP Grade (ab2765)
      Western blot - Anti-Progesterone Receptor antibody [Alpha PR6] - ChIP Grade (ab2765)
      All lanes : Anti-Progesterone Receptor antibody [Alpha PR6] (ab2765) at 1 µg/ml

      Lane 1 : T47D cell lysate untreated (-)
      Lane 2 : T47D cell lysate stimulated (+) with 100 nm promegestone for 1 hour

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat anti-Mouse IgG-HRP at 1/2000 dilution

      Predicted band size: 99 kDa

    • Flow Cytometry - Anti-Progesterone Receptor antibody [Alpha PR6] - ChIP Grade (ab2765)
      Flow Cytometry - Anti-Progesterone Receptor antibody [Alpha PR6] - ChIP Grade (ab2765)
      Overlay histogram showing T47D cells stained with ab2765 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab2765, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    文献 (34)

    发表研究结果有使用 ab2765？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab2765 被引用在 34 文献中.

    • Zhang D  et al. Cysteine dioxygenase and taurine are essential for embryo implantation by involving in E2-ERα and P4-PR signaling in mouse. J Anim Sci Biotechnol 14:6 (2023). PubMed: 36604722
    • Namiki T  et al. Uterine epithelial Gp130 orchestrates hormone response and epithelial remodeling for successful embryo attachment in mice. Sci Rep 13:854 (2023). PubMed: 36646738
    • Cheng Y  et al. NrCAM secreted by endometrial stromal cells enhances the progestin sensitivity of endometrial cancer cells through epigenetic modulation of PRB. Cancer Gene Ther 29:1452-1462 (2022). PubMed: 35388173
    • Xin L  et al. An Acellular Scaffold Facilitates Endometrial Regeneration and Fertility Restoration via Recruiting Endogenous Mesenchymal Stem Cells. Adv Healthc Mater 11:e2201680 (2022). PubMed: 36049781
    • Hammoudeh SM  et al. Enriched transcriptome analysis of laser capture microdissected populations of single cells to investigate intracellular heterogeneity in immunostained FFPE sections. Comput Struct Biotechnol J 19:5198-5209 (2021). PubMed: 34745451
    View all Publications for this product

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    Immunocytochemistry/ Immunofluorescence abreview for Anti-Progesterone Receptor antibody [Alpha PR6] - ChIP Grade

     Good
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Sheep Cell (fetal sheep cells)
    Permeabilization
    No
    Specification
    fetal sheep cells
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: RT°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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    提交于 Jan 29 2020

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