AB271997

重组Anti-Progesterone抗体[EPPTX-R1] - BSA and Azide free

Anti-Progesterone antibody [EPPTX-R1] - BSA and Azide free

RabMAb
Recombinant
了解详情

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Rabbit Recombinant Monoclonal Progesterone antibody. Carrier free. Suitable for cELISA and reacts with Chemical samples.

查看别名

Pregn 4 ene 3 20 dione

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Competitive ELISA - Anti-Progesterone antibody [EPPTX-R1] - BSA and Azide free (AB271997)

cELISA

Supplier Data

Competitive ELISA - Anti-Progesterone antibody [EPPTX-R1] - BSA and Azide free (AB271997)

ab215527 is specific to Progesterone and the percent cross reactivity with other compounds is demonstrated in the table.

This data was kindly provided by Pantex Division of Bio-Analysis, Inc.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab215527).

cELISA

Supplier Data

Competitive ELISA - Anti-Progesterone antibody [EPPTX-R1] - BSA and Azide free (AB271997)

Goat anti-rabbit IgG was coated onto a 96-well plate. Serial dilution of Progesterone (0, 10, 25, 50, 100, 300, 1000 pg/ml) was added (50 μl). This was followed by adding 25 μl HRP-conjugated Progesterone and 3-5 ng/ml of ab215527 (50 μl) into each well. Plates were incubated for 2 hours at room temperature by shaking. After washing, 125 μl Tetramethylbenzidine (TMB) substrate was added and incubated for 30 minutes at room temperature for color development. Then 125 ul of stopping solution (acid solution) was dispensed and OD was read at 450 nm on a microplate reader within 10 minutes.

This data was kindly provided by Pantex Division of Bio-Analysis, Inc.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab215527).

不同偶联物与剂型 (2)

Unconjugated

Anti-Progesterone antibody [EPPTX-R1]
Carrier free

Anti-Progesterone antibody [EPPTX-R1] - Low endotoxin, Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPPTX-R1

亚型

IgG

不含载体蛋白

Yes

应用

cELISA

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "cELISA" : {"fullname" : "Competitive ELISA", "shortname":"cELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Chemical": { "cELISA-species-checked": "testedAndGuaranteed", "cELISA-species-dilution-info": "", "cELISA-species-notes": "<p>Use 3-5 ng/ml.</p>" } } }

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产品详情

ab271997 is the carrier-free version of ab215527.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Constituents: PBS

运输条件

Blue Ice

储存信息

Do Not Freeze

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Progesterone also called P4 is a steroid hormone playing an important role in the reproductive system. Its molecular weight is approximately 314.47 Da. This hormone is mainly expressed in the ovaries but also in the adrenal glands and placenta. Progesterone acts by binding to its receptor the progesterone receptor (PR) which is found in target tissues such as the uterus mammary glands and the brain. When progesterone binds to PR it translocates to the nucleus to act as a transcription factor regulating gene expression that is important for reproductive function.

Biological function summary

Progesterone is essential for preparation and maintenance of pregnancy. It facilitates the transformation of the endometrium to a secretory phase to support embryo implantation. It also modulates immune responses during pregnancy to prevent the maternal immune system from rejecting the fetus. Progesterone and its receptor are not part of a larger protein complex but they do interact with other molecular pathways to exert their actions. Additionally progesterone inhibits the proliferation of mammary gland epithelial cells contributing to breast development and function.

Pathways

Progesterone plays a significant role in the menstrual cycle and pregnancy-related pathways. It works within the ovarian steroidogenesis pathway which includes other hormones like estrogen and luteinizing hormone. This pathway ensures the release and regulation of female sex hormones. Progesterone also interacts with the MAPK signaling pathway which involves proteins such as ERK1/2 affecting cellular processes including cell growth and differentiation.

Progesterone is linked to conditions such as endometriosis and breast cancer. In endometriosis progesterone resistance leads to the abnormal growth of endometrial tissue outside the uterus. The hormone's association with breast cancer involves its interaction with proteins like estrogen receptor alpha which can promote cancer cell proliferation when dysregulated. Understanding progesterone’s role in disease helps in developing therapeutic strategies to manage these conditions.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

See full target information Progesterone

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重组Anti-Progesterone抗体[EPPTX-R1] - BSA and Azide free