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Anti-ProDynorphin抗体(ab11137)

Reviews (3)Q&A (2)References (5)

Key features and details

  • Rabbit polyclonal to Dynorphin B
  • Suitable for: IHC-Fr
  • Reacts with: Mouse, Rat
  • Isotype: IgG

概述

  • 产品名称

    Anti-ProDynorphin抗体

  • 描述

    兔多克隆抗体to Dynorphin B

  • 宿主

    Rabbit

  • 特异性

    This antibody blocks specifically with leumorphin (rat only). Cells are found in the paraventricular and supraoptic nucleus and fibres in the lateral hypothalamus in rat brain of colchicine treated 4% FA perfused tissue.

  • 经测试应用

    适用于: IHC-Frmore details

  • 种属反应性

    与反应: Mouse, Rat

  • 免疫原

    Synthetic peptide corresponding to Rat ProDynorphin.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • 常规说明

    This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab11137于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-Fr
1/100 - 1/2000.
说明
IHC-Fr
1/100 - 1/2000.

靶标

  • 功能

    Leu-enkephalins compete with and mimic the effects of opiate drugs. They play a role in a number of physiologic functions, including pain perception and responses to stress.
    Dynorphin peptides differentially regulate the kappa opioid receptor. Dynorphin A(1-13) has a typical opiod activity, it is 700 times more potent than Leu-enkephalin.
    Leumorphin has a typical opiod activity and may have anti-apoptotic effect.

  • 疾病相关

    Defects in PDYN are the cause of spinocerebellar ataxia type 23 (SCA23) [MIM:610245]. Spinocerebellar ataxia is a clinically and genetically heterogeneous group of cerebellar disorders. Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements, due to degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. SCA23 is an adult-onset autosomal dominant form characterized by slowly progressive gait and limb ataxia, with variable additional features, including peripheral neuropathy and dysarthria.

  • 序列相似性

    Belongs to the opioid neuropeptide precursor family.

  • 翻译后修饰

    The N-terminal domain contains 6 conserved cysteines thought to be involved in disulfide bonding and/or processing.

  • 细胞定位

    Secreted.
  • Information by UniProt

  • 数据库链接

    • 别名

      • Beta-neoendorphin-dynorphin antibody
      • Big Dyn antibody
      • Dyn B antibody
      • Dyn-A17 antibody
      • Dyn-B antibody
      • Dynorphin A antibody
      • Dynorphin B antibody
      • Dynorphin B(1-13) antibody
      • Dynorphin B-29 antibody
      • Leumorphin antibody
      • PDYN antibody
      • PDYN_HUMAN antibody
      • Preprodynorphin antibody
      see all

    文献 (5)

    发表研究结果有使用 ab11137？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab11137 被引用在 5 文献中.

    • Lee GJ  et al. Anterior Insular-nucleus Accumbens Pathway Controls Refeeding-induced Analgesia under Chronic Inflammatory Pain Condition. Neuroscience 495:58-73 (2022). PubMed: 35643248
    • Liu L  et al. Dynorphin/KOR inhibits neuronal autophagy by activating mTOR signaling pathway to prevent acute seizure epilepsy. Cell Biol Int 46:1814-1824 (2022). PubMed: 35989483
    • Whitfield TW  et al. ? Opioid receptors in the nucleus accumbens shell mediate escalation of methamphetamine intake. J Neurosci 35:4296-305 (2015). PubMed: 25762676
    • Romero-Picó A  et al. Hypothalamic ?-opioid receptor modulates the orexigenic effect of ghrelin. Neuropsychopharmacology 38:1296-307 (2013). PubMed: 23348063
    • Schlosburg JE  et al. Long-term antagonism of ? opioid receptors prevents escalation of and increased motivation for heroin intake. J Neurosci 33:19384-92 (2013). Rat . PubMed: 24305833

    客户评价及客户问答

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    1-5 of 5 Abreviews or Q&A

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-ProDynorphin antibody

     Good
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Mouse Tissue sections (Brain)
    Antigen retrieval step
    None
    Permeabilization
    Yes - 0.3% Triton X-100
    Specification
    Brain
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Fixative
    Formalin
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Allisa Song

    Verified customer

    提交于 Feb 26 2016

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-ProDynorphin antibody

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Blocking step
    BSA+NGS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
    Antigen retrieval step
    None
    Sample
    Mouse Tissue sections (Cortex)
    Specification
    Cortex
    Permeabilization
    Yes - 0.3% Triton
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Mar 18 2014

    Immunohistochemistry - Free Floating abreview for Anti-ProDynorphin antibody

     Average
    Abreviews
    abreview image
    Application
    Immunohistochemistry free floating
    Sample
    Rat Tissue sections (Brain)
    Specification
    Brain
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Dr. Kouichi Nakamura

    Verified customer

    提交于 Mar 24 2011

    Question

    Thank you very much for your thoughtful replies.We would like to get the refund.I will visit you website often and make orders if necessary.Thanks again for your work. Best wishes,

    Read More
    Answer

    I have contacted our accounting department and you will be issued a refund for your order for ab11137. This process does take a couple of weeks and if you have any questions regarding the refund please email: accounts@abcam.com If you have any future questions, please contact us again. Have a good weekend!

    Read More

    Question

    Sorry for my delay to give you this letter as a reply about the antibody problem. Except of busying on other experiments I have to repeat testing on this Prodynorphin antibody by all ways I can try and make sure it could not be our faults. Now we doubt this antibody is not good for mouse tissue detection and we have to ask a replacement or refund. Anyway I am going to give the descriptions based on your questions as follows. ProDynorphin antibody Cat#ab11137, Lot number (located on the vial) :75613. Purchase order number: 72932R 1.The problem No staining 2.On what material are you testing the antibody in IHC? Mouse brain and spinal cord tissue. 3. How did you fix the samples? The tissue was fixed by immersion in 4% paraformaldehyde in PBS pH 7.4 for 4-6 hr at 4°C and cryoprotected in 30% sucrose PBS for 2 ds at 4°C. 4.Did you apply antigen retrieval step? Microwave Method. Slides are in 0.01 M tri-sodium citrate buffer pH 6.0 retrieved for 15 minutes once the temperature has reached 98°C. 5. How did you block the unspecific binding sites? Tissue sections were incubated for 2 hr at room temperature in a blocking solution of 2 % normal donkey serum in PBS with 0.3% Triton-X100. 6. Primary antibody specification (in which species was it raised against)? what dilution(s) have you tested this antibody? incubation time, wash steps (multiple short washes are more effective than fewer longer wash steps)? The primary antibody is a Rabbit polyclonal one. After blocking I incubated tissue overnight at 4°C in the primary antiserum and I have test this antibody by 1:200,1:400,1:500,1:1000 and 1:1500. I always use 0.01M PBS pH 7.4 3 x 10 min for washing. 7. Do you know whether the problems you are experiencing come from the secondary? What detection method are you using? The sections were processed with a biotinylated donkey anti-rabbit IgG secondary antibody (Jackson; 1:200), followed by the Cy3-cogugated streptavidin (Jackson; 1:1,000). This system has been used on many antibodies in my hand and worked very well. 8. Background staining at lease provide an image of your staining. See attached. 9. Which detection system did you use? See the item 7. I answered there. 10. Did you apply positive and negative controls along with the samples? Please specify. I used negative control along with the samples which use PBS to replace the primary antibody. 11. Did you apply positive and negative controls along with the samples? Please specify. Positive control: use tissues which have been demonstrated are rich in prodynorphin and other related molecules to test.( attached including Dorsal raphe nucleus and spinal dorsal horn.) Negative control: Instead of primary antibody, use buffer in which the primary antibody was diluted. 12. Optimization attempts .How many times have you tried the IHC? Do you obtain the same results every time? What steps have you altered? At least 5 times. Same negative results every time. Use microwave method to get antigen retrieval or try to use different second antibodies from 2 different companies. Attached 3 images. Image 1 is from what I did by using this antibody. Image 2 is a figure that I did recently. I picked it up randomly and just want to show that I really perform immunostaining a lot and I can provide more than enough similar staff from my hand. Image 3 is a image from The Journal of Neuroscience, September 10, 2003, 23(23):8370-8379 and I want to use it as an example. Best regards,

    Read More
    Answer

    Thank you for your patience and the details that you have provided us with. To our knowledge, this antibody has only been tested for application in IHC-frozen sections. We do not know how the antibody works in paraformaldehyde or formalin-fixed sections. My suggestion would be to try fixation in acetone or ethanol. However, I can offer you a refund if you wish, please let me know.

    Read More

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