Fix & Perm / Cell Fixation & Permeabilization试剂盒(Flow Cytometry) (ab185917)
概述
-
产品名称
Fix & Perm / Cell Fixation & Permeabilization试剂盒(Flow Cytometry) -
产品概述
Fix & Perm / Cell Fixation and Permeabilization Kit ab185917 is used to improve the staining of intracellular markers for flow cytometry. It is used to fix cells in suspension and then to permeabilize the cell membranes. It can be used for both cytoplasmic, mitochondrial and nuclear markers, as well as markers in other cellular organelles.
The reagents allow antibodies access to intracellular structures and leave the morphological scatter characteristics of cells intact. The formulation reduces background staining and allows the simultaneous addition of the permeabilization medium and fluorochrome labeled antibodies.
-
说明
The kit protocol is very simple:
- incubate cells with Fixation Medium for 15 min
- add PBS and spin down cells
- add Permeabilization Medium and antibody to cell pellet
- incubate for 15 min
- wash cells with PBS and analyze
The overwhelming majority of flow cytometry antibodies are suitable for use with this kit (a limited number are sensitive to the fixation step).
-
经测试应用
适用于: Flow Cytmore details
性能
-
存放说明
Store at room temperature. Please refer to protocols. -
组件 50 tests 200 tests Fixation Medium (Reagent A) 1 x 5ml 4 x 5ml Permeabilization Medium (Reagent B) 1 x 5ml 4 x 5ml -
研究领域
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab185917于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt |
Use at an assay dependent concentration.
|
说明 |
---|
Flow Cyt
Use at an assay dependent concentration. |
图片
-
Double labeling of a normal blood sample treated with ab185917, and immunostained for Lactoferrin (PE) and Lysozyme (FITC).
-
Double labeling of a normal blood sample treated with ab185917, and immunostained for CD3 (PE) and MPO-C2 (FITC).
-
Double labeling of a normal blood sample treated with ab185917, and immunostained for Lactoferrin (PE) and MPO-C2 (FITC).
-
Forward and side scatter pattern of a normal blood sample treated with ab185917.
-
Immunostaining with anti MPO-FITC conjugate of undifferentiated leukemia cells of AML type.
-
Immunostaining with CD3-PE conjugate of surface CD3-negative undifferentiated leukemia cells of T-ALL type.
-
Immunostaining with CD22-PE conjugate of undifferentiated leukemia cells of B-ALL type.
-
Flow cytometric analysis of normal bone marrow (BM) after fixation and pemeabilization using Fix and Perm kit ab185917, followed by immunostaining for the intra-cellular antigens Lactoferrin and MPO.
-
Flow cytrometric analysis of bone marrow (BM) from a leukemia patient after fixation and pemeabilization using ab185917, followed by immunostaining for lactoferrin and MPO-C2.
数据表及文件
-
SDS download
-
Datasheet download
文献 (6)
ab185917 被引用在 6 文献中.
- Baek MO et al. Self-transducible LRS-UNE-L peptide enhances muscle regeneration. J Cachexia Sarcopenia Muscle 13:1277-1288 (2022). PubMed: 35178893
- Sangree AK et al. Benchmarking of SpCas9 variants enables deeper base editor screens of BRCA1 and BCL2. Nat Commun 13:1318 (2022). PubMed: 35288574
- Li D et al. Targeting long noncoding RNA PMIF facilitates osteoprogenitor cells migrating to bone formation surface to promote bone formation during aging. Theranostics 11:5585-5604 (2021). PubMed: 33859765
- Wu J et al. Genome-Wide Screen and Validation of Microglia Pro-Inflammatory Mediators in Stroke. Aging Dis 12:786-800 (2021). PubMed: 34094642
- Aligholipour Farzani T et al. Immunological Analysis of a CCHFV mRNA Vaccine Candidate in Mouse Models. Vaccines (Basel) 7:N/A (2019). PubMed: 31527460
- Deng YP et al. Human bocavirus induces apoptosis and autophagy in human bronchial epithelial cells. Exp Ther Med 14:753-758 (2017). PubMed: 28672995