重组人BACE1蛋白(Active) (ab168075)
Key features and details
- Expression system: Insect cells
- Purity: > 90% SDS-PAGE
- Active: Yes
- Suitable for: SDS-PAGE, Functional Studies
描述
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产品名称
重组人BACE1蛋白(Active)
参阅全部 BACE1 蛋白酶 -
生物活性
Determined using a ß-Secretase Activity Assay Kit. Specific units > 120x103 units/mg. One unit is defined as the amount of enzyme that produces 1 pmol/min EDANS by hydrolyzing the substrate BVT9964 (NH2-Arg-Glu(EDANS)-Glu-Val-Asn-Leu-Asp-Ala-Glu-Phe-Lys(DABCYL)-Arg-COOH) at 37°C in 100 mM acetate buffer pH 4.0.
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纯度
> 90 % SDS-PAGE. -
表达系统
Insect cells -
Accession
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蛋白长度
Protein fragment -
无动物成分
No -
性质
Recombinant -
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种属
Human -
预测分子量
47 kDa -
氨基酸
46 to 460 -
额外的序列信息
Mature protease domain.
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技术指标
Our Abpromise guarantee covers the use of ab168075 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
SDS-PAGE
Functional Studies
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形式
Liquid -
补充说明
This product is manufactured by BioVision, an Abcam company and was previously called 7609 Active Recombinant Human beta-Secretase 1 - BACE-1. 7609-5 is the same size as the 5 µg size of ab168075.
Produced using non-baculovirus insect cells.
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Concentration information loading...
制备和贮存
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稳定性和存储
Shipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Constituents: 50% Glycerol (glycerin, glycerine), PBS
This product is an active protein and may elicit a biological response in vivo, handle with caution.
常规信息
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别名
- APP beta secretase
- Asp 2
- ASP2
see all -
功能
Responsible for the proteolytic processing of the amyloid precursor protein (APP). Cleaves at the N-terminus of the A-beta peptide sequence, between residues 671 and 672 of APP, leads to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase. -
组织特异性
Expressed at high levels in the brain and pancreas. In the brain, expression is highest in the substantia nigra, locus coruleus and medulla oblongata. -
序列相似性
Belongs to the peptidase A1 family. -
结构域
The transmembrane domain is necessary for its activity. It determines its late Golgi localization and access to its substrate, APP. -
翻译后修饰
Glycosylated. -
细胞定位
Membrane. Golgi apparatus > trans-Golgi network. Endoplasmic reticulum. Endosome. Cell surface. Predominantly localized to the later Golgi/trans-Golgi network (TGN) and minimally detectable in the early Golgi compartments. A small portion is also found in the endoplasmic reticulum, endosomes and on the cell surface. - Information by UniProt
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab168075 尚未被引用在任何文献中。