AFM positive control ChIP引物pair (ab269259)
概述
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产品名称
AFM positive control ChIP引物pair -
常规说明
Positive control ChIP-qPCR 5' and 3' primers for AFM gene. Use with SYBR green.
We recommend these primers as a positive control (based on Abcam's testing) for the Histone H3 dimethyl K9 histone mark. They may also be useful for other histone marks.
500pmole of each oligo per unit (lyophilised). HPLC purified, desalted and lyophilised as a sodium salt.
Quantity provided is sufficient for approx. 200 reactions based on 2.5pmol of primer per reaction with a final concentration of 100nM in 25µl.
Please contact us after purchase if you require the sequence of the oligos.
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经测试应用
适用于: ChIPmore details
性能
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形式
Lyophilized -
存放说明
Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Concentration information loading...
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研究领域
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功能
Vitamin E binding protein. May transport vitamin E in body fluids under conditions where the lipoprotein system is not sufficient or across the blood-brain barrier. -
序列相似性
Belongs to the ALB/AFP/VDB family.
Contains 3 albumin domains. -
翻译后修饰
N-glycosylated. -
细胞定位
Secreted. - Information by UniProt
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别名
- AFAM_HUMAN
- Afamin
- Afm
see all
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab269259于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ChIP |
Use at an assay dependent concentration.
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说明 |
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ChIP
Use at an assay dependent concentration. |
图片
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Chromatin was prepared from U2OS cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10min. The ChIP was performed with 25µg of chromatin, 2µg of ab1220 (blue), and 20µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci). Primers and probes are located in the first Kb of the transcribed region.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (0)
ab269259 尚未被引用在任何文献中。