AB285115

重组Anti-Procollagen III N-terminal propeptide (PIIINP)抗体[EPR24255-101] - BSA and Azide free (Detector)

Anti-Procollagen III N-terminal propeptide (PIIINP) antibody [EPR24255-101] - BSA and Azide free (Detector)

RabMAb
Recombinant
了解详情

Be the first to review this product! Submit a review

(0 Publication)

Rabbit Recombinant Monoclonal Collagen III antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

查看别名

Collagen alpha-1(III) chain, COL3A1

1 Images

Sandwich ELISA - Anti-Procollagen III N-terminal propeptide (PIIINP) antibody [EPR24255-101] - BSA and Azide free (Detector) (AB285115)

sELISA

Lab

Sandwich ELISA - Anti-Procollagen III N-terminal propeptide (PIIINP) antibody [EPR24255-101] - BSA and Azide free (Detector) (AB285115)

Sandwich ELISA of ab253842 with the capture antibody ab285009 dilution at 2 µg/mL and detector antibody ab285115 dilution at 0.1 µg/mL.

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR24255-101

亚型

IgG

不含载体蛋白

Yes

反应种属

Human

应用

sELISA

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }

style

left-column

产品详情

ab285115 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human Collagen III. The recommended pair for sandwich ELISA is:
Capture: ab285009, Human Procollagen III N-terminal propeptide (PIIINP) Capture Antibody (unconjugated)
Detector: ab285115, Procollagen III N-terminal propeptide (PIIINP) Detector Antibody (unconjugated)
The reference range value is 39.1-5000 pg/mL.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

性能和储存信息

形式

Liquid

存储溶液

Constituents: PBS

运输条件

Blue Ice

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Procollagen III N-terminal propeptide known as PIIINP serves as an important marker in collagen synthesis processes. PIIINP results from the cleavage of procollagen III and functions as a byproduct of the maturation of collagen molecules. It possesses a molecular weight of approximately 42 kDa. This target is initially expressed within fibroblast cells found in connective tissues and then secreted into the bloodstream. Given its role in collagen synthesis it is frequently measured to assess the turnover and formation of collagen in tissues.

Biological function summary

This propeptide plays a significant role in the formation of the extracellular matrix. PIIINP is not part of a protein complex but accumulates during the conversion of procollagen into mature collagen fibers which later support tissue structure. Its presence is essential for proper collagen deposition and tissue remodeling. Through its involvement in collagen synthesis PIIINP helps maintain the structural integrity of connective tissues across various organs.

Pathways

Its role reflects the broader functions of collagen metabolism and tissue remodeling pathways. PIIINP marks the processing pathway of procollagen to collagen which is important for the formation of the extracellular matrix. This pathway sometimes intersects with pathways involving other collagens such as collagen I which is another critical component of connective tissues. Together PIIINP and collagen I contribute to the proper maintenance of the extracellular matrix and the mechanical properties of tissues.

Elevated levels of PIIINP often relate to fibrotic conditions and can serve as a biomarker for diseases like liver fibrosis and systemic sclerosis. In liver fibrosis excessive collagen deposition and remodeling occur affecting liver function. In systemic sclerosis abnormal collagen synthesis and deposition in the skin cause thickening and hardening. PIIINP connection to these conditions often involves collagen-binding proteins which regulate collagen stability and deposition.

style

left-column

style

left-column

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

靶点信息

Collagen type III occurs in most soft connective tissues along with type I collagen. Involved in regulation of cortical development. Is the major ligand of ADGRG1 in the developing brain and binding to ADGRG1 inhibits neuronal migration and activates the RhoA pathway by coupling ADGRG1 to GNA13 and possibly GNA12.

See full target information COL3A1

style

left-column

style

left-column

style

right-column

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

重组Anti-Procollagen III N-terminal propeptide (PIIINP)抗体[EPR24255-101] - BSA and Azide free (Detector)

Anti-Procollagen III N-terminal propeptide (PIIINP) antibody [EPR24255-101] - BSA and Azide free (Detector)

Sandwich ELISA - Anti-Procollagen III N-terminal propeptide (PIIINP) antibody [EPR24255-101] - BSA and Azide free (Detector) (AB285115)

关键信息

宿主种属

克隆

克隆号

亚型

不含载体蛋白

反应种属

应用

免疫原