重组Anti-PRKX抗体[EPR4840] (ab109389)
![Western blot - Anti-PRKX antibody [EPR4840] (ab109389)](https://www.abcam.cn/ps/products/109/ab109389/Images/ab109389-122188-anti-prkx-antibody-epr4840-western-blot.jpg "Western blot - Anti-PRKX antibody [EPR4840] (ab109389)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4840] to PRKX
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-PRKX抗体[EPR4840]
参阅全部 PRKX 一抗 -
描述
兔单克隆抗体[EPR4840] to PRKX -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: IHC-P or IP -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Flow Cyt: permeabilized A549 cells; WB: A549, Jurkat, 293T, fetal kidney and fetal brain lysates.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR4840 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab109389于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 41 kDa.
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| 说明 |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 41 kDa. |
应用说明
Is unsuitable for IHC-P or IP.
靶标
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组织特异性
High levels in adult and fetal brain, kidney and lung; low levels in adult placenta, heart, liver, skeletal muscle, pancreas and fetal liver. -
疾病相关
Note=A chromosomal aberration involving PRKX is a cause of sex reversal disorder. Translocation t(X;Y)(p22;p11) with PRKY. Chromosomal translocations proximal to PRKY account for about 30% of the cases of sex reversal disorder in XX males and XY females. -
序列相似性
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. cAMP subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 protein kinase domain. - Information by UniProt
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数据库链接
- Entrez Gene: 5613 Human
- Entrez Gene: 19108 Mouse
- Entrez Gene: 501563 Rat
- Omim: 300083 Human
- SwissProt: P51817 Human
- SwissProt: Q922R0 Mouse
- Unigene: 390788 Human
- Unigene: 290338 Mouse
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别名
- PKX1 antibody
- PRKX antibody
- PRKX_HUMAN antibody
see all
图片
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Western blot - Anti-PRKX antibody [EPR4840] (ab109389)All lanes : Anti-PRKX antibody [EPR4840] (ab109389) at 1/1000 dilution
Lane 1 : A549 cell lysates
Lane 2 : Jurkat cell lysates
Lane 3 : 293T cell lysates
Lane 4 : Human fetal kidney lysates
Lane 5 : Human fetal brain lysates
Lysates/proteins at 10 µg per lane.
Predicted band size: 41 kDa -
![Anti-PRKX antibody [EPR4840] (ab109389)](https://www.abcam.cn/ps/products/109/ab109389/Images/ab109389-3-benefits-of-recombinant-antibodies.png)
Anti-PRKX antibody [EPR4840] (ab109389)
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab109389 被引用在 2 文献中.
- Tang B et al. PEA15 promotes liver metastasis of colorectal cancer by upregulating the ERK/MAPK signaling pathway. Oncol Rep 41:43-56 (2019). PubMed: 30365128
- Ouyang C et al. Transforming Growth Factor (TGF)-ß-activated Kinase 1 (TAK1) Activation Requires Phosphorylation of Serine 412 by Protein Kinase A Catalytic Subunit a (PKACa) and X-linked Protein Kinase (PRKX). J Biol Chem 289:24226-37 (2014). WB . PubMed: 25028512