Anti-PRKRIR抗体(ab70334)
Key features and details
- Rabbit polyclonal to PRKRIR
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-PRKRIR抗体
参阅全部 PRKRIR 一抗 -
描述
兔多克隆抗体to PRKRIR -
宿主
Rabbit -
经测试应用
适用于: WB, IP, IHC-Pmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat, Rabbit, Horse, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
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免疫原
Synthetic peptide corresponding to Human PRKRIR. Synthetic peptide (Human) corresponding to a region between residues 150 and 200 of human PRKRIR (NP_004696).
Database link: O43422 -
阳性对照
- Whole cell lysate from HeLa cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab70334于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/2000 - 1/10000. Detects a band of approximately 88 kDa (predicted molecular weight: 88 kDa).
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| IP |
Use a concentration of 1 - 4 µg/ml.
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| IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 说明 |
|---|
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WB
1/2000 - 1/10000. Detects a band of approximately 88 kDa (predicted molecular weight: 88 kDa). |
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IP
Use a concentration of 1 - 4 µg/ml. |
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IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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相关性
PRKRIR is upstream regulator of interferon induced serine/threonine protein kinase R (PKR). It may block the PKR inhibitory function of P58IPK, resulting in the restoration of kinase activity and suppression of cell growth. -
细胞定位
Nucleus -
数据库链接
- Entrez Gene: 5612 Human
- Entrez Gene: 72981 Mouse
- Entrez Gene: 308845 Rat
- Omim: 607374 Human
- SwissProt: O43422 Human
- SwissProt: Q9CUX1 Mouse
- SwissProt: Q9R0T3 Rat
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别名
- 52 kDa repressor of the inhibitor of the protein kinase antibody
- 58 kDa interferon induced protein kinase interacting protein antibody
- DAP4 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRKRIR antibody (ab70334)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma (left) and mouse squamous cell carcinoma (right) tissues labelling PRKRIR with ab70334 at 1/1000 (0.2µg/ml) and 1/200 (1µg/ml). Detection: DAB.
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Western blot - Anti-PRKRIR antibody (ab70334)All lanes : Anti-PRKRIR antibody (ab70334) at 0.04 µg/ml
Lane 1 : whole cell lysate of HeLa cells at 50 µg
Lane 2 : whole cell lysate of HeLa cells at 15 µg
Lane 3 : whole cell lysate of HeLa cells at 5 µg
Lane 4 : whole cell lysate of NIH3T3 cells at 50 µg
Predicted band size: 88 kDa
Observed band size: 88 kDa -
Immunoprecipitation - Anti-PRKRIR antibody (ab70334)IP/WB - 1 mg whole cell lysate of HeLa cells used, and 1/4 of the reaction loaded.
Lane 1: ab70333 used at 3µg/ml lysate for IP.
Lane 2: ab70334 used at 3µg/ml lysate for IP.
Lane 3: IgG control.
ab70334 used at at 1µg/ml for the subsequent WB for all lanes.
Detection: Chemiluminescence with an exposure time of 30 seconds.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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