重组Anti-PRKACG抗体[EP2647Y] (ab108385)
![Western blot - Anti-PRKACG antibody [EP2647Y] (ab108385)](https://www.abcam.cn/ps/products/108/ab108385/Images/ab108385-553914-anti-prkacg-antibody-ep2647y-western-blot-cell-lysates-human.jpg "Western blot - Anti-PRKACG antibody [EP2647Y] (ab108385)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP2647Y] to PRKACG
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-PRKACG抗体[EP2647Y]
参阅全部 PRKACG 一抗 -
描述
兔单克隆抗体[EP2647Y] to PRKACG -
宿主
Rabbit -
特异性
This antibody is likely to react with the alpha subunit, as it possesses 85.7% similar homology with the immunogen. Additionally, it may react with the beta subunit, possessing 78.6% homology.
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经测试应用
适用于: WBmore details
不适用于: ICC/IF or IHC-P -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: HeLa and PC-3 cell lysates.
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常规说明
The production method for this product has been changed from hybridoma to recombinant on 27th September 2024.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP2647Y -
同种型
IgG -
研究领域
相关产品
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab108385于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) |
1/10000. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).
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| 说明 |
|---|
|
WB
1/10000. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa). |
应用说明
Is unsuitable for ICC/IF or IHC-P.
靶标
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功能
Phosphorylates a large number of substrates in the cytoplasm and the nucleus. -
组织特异性
Testis specific. But important tissues such as brain and ovary have not been analyzed for the content of transcript. -
序列相似性
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. cAMP subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 protein kinase domain. - Information by UniProt
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数据库链接
- Entrez Gene: 5568 Human
- Omim: 176893 Human
- SwissProt: P22612 Human
- Unigene: 158029 Human
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别名
- cAMP-dependent protein kinase catalytic subunit gamma antibody
- KAPCG_HUMAN antibody
- KAPG antibody
see all
图片
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Western blot - Anti-PRKACG antibody [EP2647Y] (ab108385)All lanes : Anti-PRKACG antibody [EP2647Y] (ab108385) at 1/10000 dilution
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : PC-3 (Human prostate adenocarcinoma epithelial cell) whole cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 40 kDa
Exposure time: 40 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST.
数据表及文件
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SDS download
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Datasheet download
文献 (7)
ab108385 被引用在 7 文献中.
- Song C et al. Eleutheroside E supplementation prevents radiation-induced cognitive impairment and activates PKA signaling via gut microbiota. Commun Biol 5:680 (2022). PubMed: 35804021
- Ye J et al. Tau inhibits PKA by nuclear proteasome-dependent PKAR2a elevation with suppressed CREB/GluA1 phosphorylation. Aging Cell 19:e13055 (2020). PubMed: 31668016
- Reis MB et al. Interleukin-1 receptor-induced PGE2 production controls acetylcholine-mediated cardiac dysfunction and mortality during scorpion envenomation. Nat Commun 11:5433 (2020). PubMed: 33116136
- Yin C et al. PID1 alters the antilipolytic action of insulin and increases lipolysis via inhibition of AKT/PKA pathway activation. PLoS One 14:e0214606 (2019). PubMed: 30990811
- Cao W et al. Hoxa5 Promotes Adipose Differentiation via Increasing DNA Methylation Level and Inhibiting PKA/HSL Signal Pathway in Mice. Cell Physiol Biochem 45:1023-1033 (2018). PubMed: 29439250
- Liu G et al. ColXV promotes adipocyte differentiation via inhibiting DNA methylation and cAMP/PKA pathway in mice. Oncotarget 8:60135-60148 (2017). PubMed: 28947959
- Cao N et al. Clinical-grade human umbilical cord-derived mesenchymal stem cells reverse cognitive aging via improving synaptic plasticity and endogenous neurogenesis. Cell Death Dis 8:e2996 (2017). WB . PubMed: 28796260