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Neuroscience Neurology process Neurodegenerative disease Prions

Anti-Prion蛋白PrP抗体[F89/160.1.5] (ab2777)

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Flow Cytometry - Anti-Prion protein PrP antibody [F89/160.1.5] (ab2777)

    Key features and details

    • Mouse monoclonal [F89/160.1.5] to Prion protein PrP
    • Suitable for: Flow Cyt
    • Reacts with: Human
    • Isotype: IgG1

    选择批间可重复性更高的重组抗体

    Product image
    Anti-Prion protein PrP antibody [EP1802Y] (ab52604)
    • 研究可靠 —— 各批次间结果一致且可重复
    • 长期批量供应 —— 采用重组技术,可实现快速生产
    • 首次实验即可成功 —— 经过大量验证确认了特异性
    • 符合伦理标准 —— 产品不含动物成分

    概述

    • 产品名称

      Anti-Prion蛋白PrP抗体[F89/160.1.5]
      参阅全部 Prion protein PrP 一抗
    • 描述

      小鼠单克隆抗体[F89/160.1.5] to Prion蛋白PrP
    • 宿主

      Mouse
    • 经测试应用

      适用于: Flow Cytmore details
    • 种属反应性

      与反应: Human
      预测可用于: Pig
    • 免疫原

      Synthetic peptide corresponding to Cow Prion protein PrP aa 146-159. U.S. Patent Number: 4,806,627.
      Sequence:

      SRPLIHFGSDYEDR


      (Peptide available as ab4908)
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    • 表位

      This antibody is known to specifically recognize a conserved epitope of the PrP(Sc) protein comprising the amino acids n-IHFG-c.
    • 常规说明

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    • 形式

      Liquid
    • 存放说明

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • 存储溶液

      Preservative: 0.05% Sodium azide
      Constituent: 0.1% BSA
    • Concentration information loading...
    • 纯度

      Protein G purified
    • 克隆

      单克隆
    • 克隆编号

      F89/160.1.5
    • 同种型

      IgG1
    • 研究领域

      • Neuroscience
      • Neurology process
      • Neurodegenerative disease
      • Prions
      • Stem Cells
      • Hematopoietic Progenitors
      • Surface Molecules
      • Neuroscience
      • Diseases

    相关产品

    • Compatible Secondaries

      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
      • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Conjugation kits

      • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
      • APC Conjugation Kit - Lightning-Link® (ab201807)
    • Isotype control

      • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
      • Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353)
    • Recombinant Protein

      • Recombinant Human Prion protein PrP (denatured) (ab140567)

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab2777于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    Flow Cyt
    Use 0.1µg for 106 cells.

    ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. 

    说明
    Flow Cyt
    Use 0.1µg for 106 cells.

    ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. 

    靶标

    • 功能

      The function of PrP is still under debate. May play a role in neuronal development and synaptic plasticity. May be required for neuronal myelin sheath maintenance. May play a role in iron uptake and iron homeostasis (By similarity). Isoform 2 may act as a growth suppressor by arresting the cell cycle at the G0/G1 phase. Soluble oligomers are toxic to cultured neuroblastoma cells and induce apoptosis (in vitro).
    • 疾病相关

      Note=PrP is found in high quantity in the brain of humans and animals infected with neurodegenerative diseases known as transmissible spongiform encephalopathies or prion diseases, like: Creutzfeldt-Jakob disease (CJD), fatal familial insomnia (FFI), Gerstmann-Straussler disease (GSD), Huntington disease-like type 1 (HDL1) and kuru in humans; scrapie in sheep and goat; bovine spongiform encephalopathy (BSE) in cattle; transmissible mink encephalopathy (TME); chronic wasting disease (CWD) of mule deer and elk; feline spongiform encephalopathy (FSE) in cats and exotic ungulate encephalopathy (EUE) in nyala and greater kudu. The prion diseases illustrate three manifestations of CNS degeneration: (1) infectious (2) sporadic and (3) dominantly inherited forms. TME, CWD, BSE, FSE, EUE are all thought to occur after consumption of prion-infected foodstuffs.
      Defects in PRNP are the cause of Creutzfeldt-Jakob disease (CJD) [MIM:123400]. CJD occurs primarily as a sporadic disorder (1 per million), while 10-15% are familial. Accidental transmission of CJD to humans appears to be iatrogenic (contaminated human growth hormone (HGH), corneal transplantation, electroencephalographic electrode implantation, etc.). Epidemiologic studies have failed to implicate the ingestion of infected annimal meat in the pathogenesis of CJD in human. The triad of microscopic features that characterize the prion diseases consists of (1) spongiform degeneration of neurons, (2) severe astrocytic gliosis that often appears to be out of proportion to the degree of nerve cell loss, and (3) amyloid plaque formation. CJD is characterized by progressive dementia and myoclonic seizures, affecting adults in mid-life. Some patients present sleep disorders, abnormalities of high cortical function, cerebellar and corticospinal disturbances. The disease ends in death after a 3-12 months illness.
      Defects in PRNP are the cause of fatal familial insomnia (FFI) [MIM:600072]. FFI is an autosomal dominant disorder and is characterized by neuronal degeneration limited to selected thalamic nuclei and progressive insomnia.
      Defects in PRNP are the cause of Gerstmann-Straussler disease (GSD) [MIM:137440]. GSD is a heterogeneous disorder and was defined as a spinocerebellar ataxia with dementia and plaquelike deposits. GSD incidence is less than 2 per 100 million live births.
      Defects in PRNP are the cause of Huntington disease-like type 1 (HDL1) [MIM:603218]. HDL1 is an autosomal dominant, early onset neurodegenerative disorder with prominent psychiatric features.
      Defects in PRNP are the cause of kuru (KURU) [MIM:245300]. Kuru is transmitted during ritualistic cannibalism, among natives of the New Guinea highlands. Patients exhibit various movement disorders like cerebellar abnormalities, rigidity of the limbs, and clonus. Emotional lability is present, and dementia is conspicuously absent. Death usually occurs from 3 to 12 month after onset.
      Defects in PRNP are the cause of spongiform encephalopathy with neuropsychiatric features (SENF) [MIM:606688]; an autosomal dominant presenile dementia with a rapidly progressive and protracted clinical course. The dementia was characterized clinically by frontotemporal features, including early personality changes. Some patients had memory loss, several showed aggressiveness, hyperorality and verbal stereotypy, others had parkinsonian symptoms.
    • 序列相似性

      Belongs to the prion family.
    • 结构域

      The normal, monomeric form has a mainly alpha-helical structure. The disease-associated, protease-resistant form forms amyloid fibrils containing a cross-beta spine, formed by a steric zipper of superposed beta-strands. Disease mutations may favor intermolecular contacts via short beta strands, and may thereby trigger oligomerization.
      Contains an N-terminal region composed of octamer repeats. At low copper concentrations, the sidechains of His residues from three or four repeats contribute to the binding of a single copper ion. Alternatively, a copper ion can be bound by interaction with the sidechain and backbone amide nitrogen of a single His residue. The observed copper binding stoichiometry suggests that two repeat regions cooperate to stabilize the binding of a single copper ion. At higher copper concentrations, each octamer can bind one copper ion by interactions with the His sidechain and Gly backbone atoms. A mixture of binding types may occur, especially in the case of octamer repeat expansion. Copper binding may stabilize the conformation of this region and may promote oligomerization.
    • 翻译后修饰

      The glycosylation pattern (the amount of mono-, di- and non-glycosylated forms or glycoforms) seems to differ in normal and CJD prion.
      Isoform 2 is sumoylated by SUMO1.
    • 细胞定位

      Cell membrane. Golgi apparatus and Cytoplasm. Nucleus. Accumulates outside the secretory route in the cytoplasm, from where it relocates to the nucleus.
    • Target information above from: UniProt accession P04156 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 数据库链接

      • Entrez Gene: 5621 Human
      • Entrez Gene: 494014 Pig
      • Omim: 176640 Human
      • SwissProt: P04156 Human
      • Unigene: 472010 Human
      • Unigene: 610285 Human
      • Unigene: 727471 Human
      • 别名

        • Alternative prion protein; major prion protein antibody
        • AltPrP antibody
        • ASCR antibody
        • CD230 antibody
        • CD230 antigen antibody
        • CJD antibody
        • GSS antibody
        • KURU antibody
        • Major prion protein antibody
        • p27 30 antibody
        • PRIO_HUMAN antibody
        • Prion protein antibody
        • Prion related protein antibody
        • PRIP antibody
        • PRNP antibody
        • PrP antibody
        • PrP27 30 antibody
        • PrP27-30 antibody
        • PrP33-35C antibody
        • PrPC antibody
        • PrPSc antibody
        • Sinc antibody
        see all

      图片

      • Flow Cytometry - Anti-Prion protein PrP antibody [F89/160.1.5] (ab2777)
        Flow Cytometry - Anti-Prion protein PrP antibody [F89/160.1.5] (ab2777)
        Overlay histogram showing SH-SH5Y cells stained with ab2777 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab2777, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SH-SY5Y cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

      实验方案

      • Flow cytometry protocols

      Click here to view the general protocols

      数据表及文件

      • Datasheet download

        Download

      文献 (2)

      发表研究结果有使用 ab2777?请让我们知道,以便我们可以引用本数据表中的参考文章。

      ab2777 被引用在 2 文献中.

      • Wang P  et al. Selection and characterization of DNA aptamers against PrP(Sc). Exp Biol Med (Maywood) 236:466-76 (2011). WB . PubMed: 21444369
      • O'Rourke KI  et al. Monoclonal antibody F89/160.1.5 defines a conserved epitope on the ruminant prion protein. J Clin Microbiol 36:1750-5 (1998). PubMed: 9620413

      客户评价及客户问答

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      1-3 of 3 Abreviews or Q&A

      Question

      I was wondering if this product could be ordered without BSA. Thanks in advance, Rob

      Read More

      Abcam community

      Verified customer

      Asked on Nov 15 2004

      Answer

      T hank you for your enquiry regarding ab2777. Unfortunately, we don't supply this product without BSA. I'm sorry for any inconvenience this may cause to you. In the future, if you cannot find what you are looking for within the Abcam online catalogue, you can click on the link to "The World's Antibody Gateway". The World's Antibody Gateway is a free search engine service provided by Abcam to help you to quickly find the antibodies that you are looking for. It is a free-text search engine developed by the Abcam team so that it searches the catalogues of all online antibody companies (currently 249). Should you require any further information, please get in touch with us and we will gladly assist you.

      Read More

      Abcam Scientific Support

      回复于 Nov 15 2004

      Question

      I have some rather difficult questions regarding your PrP antibody AB2777: - What kind of chemical link was used to couple the immunizing Peptide to its carrier? - Was the linker coupled N- or C-terminal to the peptide? - What was used as a carrier (BSA, KLH?)? - Has the antibody been tested for reactivity with its immunizing peptide? The background is, that a customer would like to build up a detection system for PrP based on capillary electrophoresis. He would like to use AB2777 in this method for binding to PrP and compete the binding with the fluorescent labeled peptide.

      Read More

      Abcam community

      Verified customer

      Asked on Sep 26 2003

      Answer

      KLH was used as a carrier but I do not know if it was linked to the N or C-terminal. This antibody has been tested with overlapping peptide sequences according to table 1 of J. Clin. Microbiol. 36 (6): 1750-1755.

      Read More

      Abcam Scientific Support

      回复于 Sep 30 2003

      Question

      We are working lately at university of kentucky, lexington, USA., on the chronic wasting disease (CWD) prion diseases of deer and elk. we are analyzing the brain and skeletal muscle tissues from these infected animals. I would appreciate greatly if you could spare some free sample of Prion protein (PrP) antibody (ab2777) [F89/160.1.5], for our studies. Based on the results, we would like to buy this antibody from Abcam for our future studies. Please let me know about the possibility of sparing some quantity of the aforesaid antibody. I look forward to your reply.

      Read More

      Abcam community

      Verified customer

      Asked on Apr 28 2003

      Answer

      We do not offer free or trial sized samples for testing purposes. Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we will offer a replacement or reimbursement. Should you decide to test an antibody in an application for which we do not have any information, please let us know how you get on and in return we will forward a GBP10/ USD15/ EUR15 Amazon gift voucher.

      Read More

      Abcam Scientific Support

      回复于 Apr 28 2003

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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