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Neuroscience Neurology process Neurodegenerative disease Prions

Anti-Prion蛋白PrP抗体[7B6 / D2] (ab2882)

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Western blot - Anti-Prion protein PrP antibody [7B6 / D2] (ab2882)

    Key features and details

    • Mouse monoclonal [7B6 / D2] to Prion protein PrP
    • Suitable for: WB
    • Reacts with: Cow
    • Isotype: IgG1

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    概述

    • 产品名称

      Anti-Prion蛋白PrP抗体[7B6 / D2]
      参阅全部 Prion protein PrP 一抗
    • 描述

      小鼠单克隆抗体[7B6 / D2] to Prion蛋白PrP
    • 宿主

      Mouse
    • 特异性

      This antibody detects: Human recombinant prion protein. Bovine recombinant, native and PrPres proteinase K treated prion protein.
    • 经测试应用

      适用于: WBmore details
    • 种属反应性

      与反应: Cow
    • 免疫原

      Recombinant full length protein corresponding to Bovine Prion protein PrP. Immunization was performed according to the protocol described by Hofmann, J. et al.

    • 表位

      This antibody has been shown to specifically bind between amino acids 123 - 136 and 140 - 160 of bovine prion protein, determined by pepscan (Osman, A.A. et al.).
    • 常规说明

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    • 形式

      Liquid
    • 存放说明

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
    • 存储溶液

      Preservative: 0.02% Sodium azide
    • Concentration information loading...
    • 纯度

      Immunogen affinity purified
    • 克隆

      单克隆
    • 克隆编号

      7B6 / D2
    • 同种型

      IgG1
    • 轻链类型

      kappa
    • 研究领域

      • Neuroscience
      • Neurology process
      • Neurodegenerative disease
      • Prions
      • Stem Cells
      • Hematopoietic Progenitors
      • Surface Molecules
      • Neuroscience
      • Diseases

    相关产品

    • Compatible Secondaries

      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
      • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Isotype control

      • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
    • Recombinant Protein

      • Recombinant Human Prion protein PrP (denatured) (ab140567)

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab2882于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    WB
    Use at an assay dependent concentration.

    Western blot (non-reducing conditions): Use at a concentration of 5 µg/ml.

    说明
    WB
    Use at an assay dependent concentration.

    Western blot (non-reducing conditions): Use at a concentration of 5 µg/ml.

    靶标

    • 功能

      The function of PrP is still under debate. May play a role in neuronal development and synaptic plasticity. May be required for neuronal myelin sheath maintenance. May play a role in iron uptake and iron homeostasis (By similarity). Isoform 2 may act as a growth suppressor by arresting the cell cycle at the G0/G1 phase. Soluble oligomers are toxic to cultured neuroblastoma cells and induce apoptosis (in vitro).
    • 疾病相关

      Note=PrP is found in high quantity in the brain of humans and animals infected with neurodegenerative diseases known as transmissible spongiform encephalopathies or prion diseases, like: Creutzfeldt-Jakob disease (CJD), fatal familial insomnia (FFI), Gerstmann-Straussler disease (GSD), Huntington disease-like type 1 (HDL1) and kuru in humans; scrapie in sheep and goat; bovine spongiform encephalopathy (BSE) in cattle; transmissible mink encephalopathy (TME); chronic wasting disease (CWD) of mule deer and elk; feline spongiform encephalopathy (FSE) in cats and exotic ungulate encephalopathy (EUE) in nyala and greater kudu. The prion diseases illustrate three manifestations of CNS degeneration: (1) infectious (2) sporadic and (3) dominantly inherited forms. TME, CWD, BSE, FSE, EUE are all thought to occur after consumption of prion-infected foodstuffs.
      Defects in PRNP are the cause of Creutzfeldt-Jakob disease (CJD) [MIM:123400]. CJD occurs primarily as a sporadic disorder (1 per million), while 10-15% are familial. Accidental transmission of CJD to humans appears to be iatrogenic (contaminated human growth hormone (HGH), corneal transplantation, electroencephalographic electrode implantation, etc.). Epidemiologic studies have failed to implicate the ingestion of infected annimal meat in the pathogenesis of CJD in human. The triad of microscopic features that characterize the prion diseases consists of (1) spongiform degeneration of neurons, (2) severe astrocytic gliosis that often appears to be out of proportion to the degree of nerve cell loss, and (3) amyloid plaque formation. CJD is characterized by progressive dementia and myoclonic seizures, affecting adults in mid-life. Some patients present sleep disorders, abnormalities of high cortical function, cerebellar and corticospinal disturbances. The disease ends in death after a 3-12 months illness.
      Defects in PRNP are the cause of fatal familial insomnia (FFI) [MIM:600072]. FFI is an autosomal dominant disorder and is characterized by neuronal degeneration limited to selected thalamic nuclei and progressive insomnia.
      Defects in PRNP are the cause of Gerstmann-Straussler disease (GSD) [MIM:137440]. GSD is a heterogeneous disorder and was defined as a spinocerebellar ataxia with dementia and plaquelike deposits. GSD incidence is less than 2 per 100 million live births.
      Defects in PRNP are the cause of Huntington disease-like type 1 (HDL1) [MIM:603218]. HDL1 is an autosomal dominant, early onset neurodegenerative disorder with prominent psychiatric features.
      Defects in PRNP are the cause of kuru (KURU) [MIM:245300]. Kuru is transmitted during ritualistic cannibalism, among natives of the New Guinea highlands. Patients exhibit various movement disorders like cerebellar abnormalities, rigidity of the limbs, and clonus. Emotional lability is present, and dementia is conspicuously absent. Death usually occurs from 3 to 12 month after onset.
      Defects in PRNP are the cause of spongiform encephalopathy with neuropsychiatric features (SENF) [MIM:606688]; an autosomal dominant presenile dementia with a rapidly progressive and protracted clinical course. The dementia was characterized clinically by frontotemporal features, including early personality changes. Some patients had memory loss, several showed aggressiveness, hyperorality and verbal stereotypy, others had parkinsonian symptoms.
    • 序列相似性

      Belongs to the prion family.
    • 结构域

      The normal, monomeric form has a mainly alpha-helical structure. The disease-associated, protease-resistant form forms amyloid fibrils containing a cross-beta spine, formed by a steric zipper of superposed beta-strands. Disease mutations may favor intermolecular contacts via short beta strands, and may thereby trigger oligomerization.
      Contains an N-terminal region composed of octamer repeats. At low copper concentrations, the sidechains of His residues from three or four repeats contribute to the binding of a single copper ion. Alternatively, a copper ion can be bound by interaction with the sidechain and backbone amide nitrogen of a single His residue. The observed copper binding stoichiometry suggests that two repeat regions cooperate to stabilize the binding of a single copper ion. At higher copper concentrations, each octamer can bind one copper ion by interactions with the His sidechain and Gly backbone atoms. A mixture of binding types may occur, especially in the case of octamer repeat expansion. Copper binding may stabilize the conformation of this region and may promote oligomerization.
    • 翻译后修饰

      The glycosylation pattern (the amount of mono-, di- and non-glycosylated forms or glycoforms) seems to differ in normal and CJD prion.
      Isoform 2 is sumoylated by SUMO1.
    • 细胞定位

      Cell membrane. Golgi apparatus and Cytoplasm. Nucleus. Accumulates outside the secretory route in the cytoplasm, from where it relocates to the nucleus.
    • Target information above from: UniProt accession P04156 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 数据库链接

      • Entrez Gene: 281427 Cow
      • SwissProt: P10279 Cow
      • 别名

        • Alternative prion protein; major prion protein antibody
        • AltPrP antibody
        • ASCR antibody
        • CD230 antibody
        • CD230 antigen antibody
        • CJD antibody
        • GSS antibody
        • KURU antibody
        • Major prion protein antibody
        • p27 30 antibody
        • PRIO_HUMAN antibody
        • Prion protein antibody
        • Prion related protein antibody
        • PRIP antibody
        • PRNP antibody
        • PrP antibody
        • PrP27 30 antibody
        • PrP27-30 antibody
        • PrP33-35C antibody
        • PrPC antibody
        • PrPSc antibody
        • Sinc antibody
        see all

      图片

      • Western blot - Anti-Prion protein PrP antibody [7B6 / D2] (ab2882)
        Western blot - Anti-Prion protein PrP antibody [7B6 / D2] (ab2882)
        ab2882 at a 1 µg/ml concentration staining ~23 kDa  (mature) and ~28 kDa (pro-peptide) native bovine prion protein from brain (M. oblongata; 100 µg comprising 10% homogenised brain, diluted 1:10 in buffer, 10µl loaded) by Western blot (non-reducing conditions).     ab2882 at a 1 µg/ml concentration staining ~23 kDa (mature) and ~28 kDa (pro-peptide) native bovine prion protein from brain (M. oblongata; 100 µg comprising 10% homogenised brain, diluted 1:10 in buffer, 10µl loaded) by Western blot (non-reducing conditions).

      实验方案

      • Western blot protocols

      Click here to view the general protocols

      数据表及文件

      • Datasheet download

        Download

      文献 (1)

      发表研究结果有使用 ab2882?请让我们知道,以便我们可以引用本数据表中的参考文章。

      ab2882 被引用在 1 文献中.

      • Varshney M  et al. Prion protein detection using nanomechanical resonator arrays and secondary mass labeling. Anal Chem 80:2141-8 (2008). ELISA . PubMed: 18271602

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