重组Anti-Presenilin 2/AD5抗体[EP1515Y] (ab51249)
![Western blot - Anti-Presenilin 2/AD5 antibody [EP1515Y] (ab51249)](https://www.abcam.cn/ps/products/51/ab51249/Images/ab51249-365457-anti-presenilin-2-ad5-antibody-ep1515y-westernblot-human-mouse-rat.jpg "Western blot - Anti-Presenilin 2/AD5 antibody [EP1515Y] (ab51249)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1515Y] to Presenilin 2/AD5
- Suitable for: Flow Cyt (Intra), WB, IHC-P, IP, ICC/IF
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-Presenilin 2/AD5抗体[EP1515Y]
参阅全部 Presenilin 2/AD5 一抗 -
描述
兔单克隆抗体[EP1515Y] to Presenilin 2/AD5 -
宿主
Rabbit -
特异性
The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
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经测试应用
适用于: Flow Cyt (Intra), WB, IHC-P, IP, ICC/IFmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human Presenilin 2/AD5 aa 300-400 (C terminal). The exact sequence is proprietary.
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阳性对照
- WB: HeLa, HepG2, C2C12, C6, and Neuro-2a lysates; IHC-P: Human cerebrum tissue; ICC/IF: PC-12 cells; Flow Cyt (intra): HeLa cells; IP: HeLa and PC-12 cell lysates.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP1515Y -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab51249于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt (Intra) |
1/50.
|
|
| WB | (4) |
1/20000. Detects a band of approximately 23 kDa (predicted molecular weight: 50 kDa).
|
| IHC-P | (1) |
1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
See IHC antigen retrieval protocols. The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. |
| IP | (1) |
1/20.
|
| ICC/IF |
1/50 - 1/250.
|
| 说明 |
|---|
|
Flow Cyt (Intra)
1/50. |
|
WB
1/20000. Detects a band of approximately 23 kDa (predicted molecular weight: 50 kDa). |
|
IHC-P
1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. See IHC antigen retrieval protocols. The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. |
|
IP
1/20. |
|
ICC/IF
1/50 - 1/250. |
靶标
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功能
Probable catalytic subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (beta-amyloid precursor protein). Requires the other members of the gamma-secretase complex to have a protease activity. May play a role in intracellular signaling and gene expression or in linking chromatin to the nuclear membrane. May function in the cytoplasmic partitioning of proteins. -
组织特异性
Isoform 1 is seen in the placenta, skeletal muscle and heart while isoform 2 is seen in the heart, brain, placenta, liver, skeletal muscle and kidney. -
疾病相关
Defects in PSEN2 are the cause of Alzheimer disease type 4 (AD4) [MIM:606889]. AD is an autosomal dominant Alzheimer disease. Alzheimer disease is a neurodegenerative disorder characterized by progressive dementia, loss of cognitive abilities, and deposition of fibrillar amyloid proteins as intraneuronal neurofibrillary tangles, extracellular amyloid plaques and vascular amyloid deposits. The major constituent of these plaques is the neurotoxic amyloid-beta-APP 40-42 peptide (s), derived proteolytically from the transmembrane precursor protein APP by sequential secretase processing. The cytotoxic C-terminal fragments (CTFs) and the caspase-cleaved products such as C31 derived from APP, are also implicated in neuronal death.
Defects in PSEN2 are the cause of cardiomyopathy dilated type 1V (CMD1V) [MIM:613697]. It is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death. -
序列相似性
Belongs to the peptidase A22A family. -
结构域
The PAL motif is required for normal active site conformation. -
翻译后修饰
Heterogeneous proteolytic processing generates N-terminal and C-terminal fragments.
Phosphorylated on serine residues. -
细胞定位
Endoplasmic reticulum membrane. Golgi apparatus membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 5664 Human
- Entrez Gene: 19165 Mouse
- Entrez Gene: 81751 Rat
- Omim: 600759 Human
- SwissProt: P49810 Human
- SwissProt: Q61144 Mouse
- SwissProt: O88777 Rat
- Unigene: 25363 Human
see all -
别名
- AD3L antibody
- AD3LP antibody
- AD4 antibody
see all
图片
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Western blot - Anti-Presenilin 2/AD5 antibody [EP1515Y] (ab51249)All lanes : Anti-Presenilin 2/AD5 antibody [EP1515Y] (ab51249) at 1/20000 dilution (Purified)
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates
Lane 3 : C2C12 (Mouse myoblasts myoblast) whole cell lysates
Lane 4 : C6 (Rat glial tumor glial cell) whole cell lysates
Lane 5 : Neuro-2a (Mouse neuroblastoma neuroblast) whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 50 kDa
Observed band size: 23 kDa why is the actual band size different from the predicted? -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Presenilin 2/AD5 antibody [EP1515Y] (ab51249)](https://www.abcam.cn/ps/products/51/ab51249/Images/ab51249-10-anti-presenilin-2-ad5-antibody-ep1515y-immunohistochemistry-cerebrum-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Presenilin 2/AD5 antibody [EP1515Y] (ab51249)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cerebrum tissue sections labeling Presenilin 2/AD5 with purified ab51249 at 1/500 dilution (0.51 µg/ml). Heat mediated antigen retrieval using BondTM Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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![Immunocytochemistry/ Immunofluorescence - Anti-Presenilin 2/AD5 antibody [EP1515Y] (ab51249)](https://www.abcam.cn/ps/products/51/ab51249/Images/ab51249-11-anti-presenilin-2-ad5-antibody-ep1515y-immunocytochemistry-pc12-rat.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Presenilin 2/AD5 antibody [EP1515Y] (ab51249)Immunocytochemistry/ Immunofluorescence analysis of PC-12 (Rat adrenal gland pheochromocytoma) cells labeling Presenilin 2/AD5 with purified ab51249 at 1/50 dilution (5 µg/ml). Cells were fixed in 100% Methanol and permeabilized with None. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control. -
![Flow Cytometry (Intracellular) - Anti-Presenilin 2/AD5 antibody [EP1515Y] (ab51249)](https://www.abcam.cn/ps/products/51/ab51249/Images/ab51249-12-anti-presenilin-2-ad5-antibody-ep1515y-flowcytometry-hela-human.jpg)
Flow Cytometry (Intracellular) - Anti-Presenilin 2/AD5 antibody [EP1515Y] (ab51249)Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Presenilin 2/AD5 with purified ab51249 at 1/50 dilution (5 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
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![Immunoprecipitation - Anti-Presenilin 2/AD5 antibody [EP1515Y] (ab51249)](https://www.abcam.cn/ps/products/51/ab51249/Images/ab51249-13-anti-presenilin-2-ad5-antibody-ep1515y-immunoprecipitation-hela-human.jpg)
Immunoprecipitation - Anti-Presenilin 2/AD5 antibody [EP1515Y] (ab51249)ab51249 (purified ) at 1/20 dilution (1ug) immunoprecipitating Presenilin 2/AD5 in HeLa whole cell lysate.
Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10ug
Lane 2 (+): ab51249 & HeLa whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab51249 in HeLa whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST. -
![Immunoprecipitation - Anti-Presenilin 2/AD5 antibody [EP1515Y] (ab51249)](https://www.abcam.cn/ps/products/51/ab51249/Images/ab51249-239034-anti-presenilin-2-ad5-antibody-ep1515y-immunoprecipitation.jpg)
Immunoprecipitation - Anti-Presenilin 2/AD5 antibody [EP1515Y] (ab51249)Presenilin was immunoprecipitated from 1 mg of HeLa whole cell extract with unpurified ab51249 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab51249 at 1/5000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.
Input: HeLa whole cell extract 10 µg.
IP (+): ab51249 IP in HeLa whole cell extract.
IP (-): Rabbit monoclonal IgG (ab172730) instead of ab51249 in HeLa whole cell extract.
Blocking and dilution buffer and concentration: 5% NFDM/TBST. -
![Immunoprecipitation - Anti-Presenilin 2/AD5 antibody [EP1515Y] (ab51249)](https://www.abcam.cn/ps/products/51/ab51249/Images/ab51249-239035-anti-presenilin-2-ad5-antibody-ep1515y-immunoprecipitation.jpg)
Immunoprecipitation - Anti-Presenilin 2/AD5 antibody [EP1515Y] (ab51249)Presenilin was immunoprecipitated from 1 mg of PC-12 (rat adrenal gland pheochromocytoma) whole cell extract with unpurified ab51249 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab51249 at 1/5000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.
Input: PC-12 whole cell extract 10 µg.
IP (+): ab51249 IP in PC-12 whole cell extract.
IP (-): Rabbit monoclonal IgG (ab172730) instead of ab51249 in PC-12 whole cell extract.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (21)
ab51249 被引用在 21 文献中.
- Gao C et al. Presenilin2 D439A Mutation Induces Dysfunction of Mitochondrial Fusion/Fission Dynamics and Abnormal Regulation of GTPase Activity. Mol Neurobiol 61:5047-5070 (2024). PubMed: 38159198
- Ulku I et al. Mechanisms of amyloid-β34 generation indicate a pivotal role for BACE1 in amyloid homeostasis. Sci Rep 13:2216 (2023). PubMed: 36750595
- Depp C et al. Myelin dysfunction drives amyloid-β deposition in models of Alzheimer's disease. Nature 618:349-357 (2023). PubMed: 37258678
- Sun Y et al. Apolipoprotein E4 inhibits γ-secretase activity via binding to the γ-secretase complex. J Neurochem 164:858-874 (2023). PubMed: 36582176
- Klein M et al. Converging roles of PSENEN/PEN2 and CLN3 in the autophagy-lysosome system. Autophagy 18:2068-2085 (2022). PubMed: 34964690