AB76114

重组Anti-Presenilin 2/AD5 (phospho S327)抗体[EP2613Y]

Anti-Presenilin 2/AD5 (phospho S327) antibody [EP2613Y]

20ul selling size
RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal Presenilin 2/AD5 phospho S327 antibody. Suitable for WB, Dot and reacts with Human, Mouse, Synthetic peptide samples. Cited in 1 publication.

查看别名

AD4, PS2, PSNL2, STM2, PSEN2, Presenilin-2, PS-2, AD3LP, AD5, E5-1, STM-2

3 Images

Supplier Data

Western blot - Anti-Presenilin 2/AD5 (phospho S327) antibody [EP2613Y] (AB76114)

All lanes:

Western blot - Anti-Presenilin 2/AD5 (phospho S327) antibody [EP2613Y] (ab76114) at 1/500 dilution

Lane 1:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 15 µg

Lane 2:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate. Then the membrane was incubated with alkaline phosphatase at 15 µg

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Exposure time: 180s

Supplier Data

Western blot - Anti-Presenilin 2/AD5 (phospho S327) antibody [EP2613Y] (AB76114)

Blocking and diluting buffer and concentration : 5% NFDM/TBST, Primary antibody : incubated for 1h at room temperature. Secondary antibody : incubated for 1h at 1 : 20,000 at room temperature (ab97051); incubated for 1h at 1 : 2000 at room temperature (Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG)

All lanes:

Western blot - Anti-Presenilin 2/AD5 (phospho S327) antibody [EP2613Y] (ab76114) at 1/500 dilution

Lane 1:

RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 15 µg

Lane 2:

RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate. Then the membrane was incubated with alkaline phosphatase at 15 µg

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Supplier Data

Dot Blot - Anti-Presenilin 2/AD5 (phospho S327) antibody [EP2613Y] (AB76114)

Dot blot analysis labelling Presenilin 2/AD5 (phospho S327) with ab76114 at 1/500 dilution. Lane 1 : Presenilin 2/AD5 (phospho S327) peptide Lane 2 : Presenilin 2/AD5 non-phospho peptide A peroxidase-conjugated goat anti-rabbit IgG (H+L) (ab97051) was used as the secondary antibody at a dilution of 1/100,000. Blocking and dilution buffer : 5% NFDM/TBST. Exposure time : 180s.

不同偶联物与剂型 (1)

Carrier free

Anti-Presenilin 2/AD5 (phospho S327) antibody [EP2613Y] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EP2613Y

亚型

IgG

不含载体蛋白

反应种属

Mouse, Human

应用

WB, Dot

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "Dot-species-checked": "predicted", "Dot-species-dilution-info": "", "Dot-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "Dot-species-checked": "predicted", "Dot-species-dilution-info": "", "Dot-species-notes": "" }, "Synthetic peptide": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "" } } }

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产品详情

This product has switched from a hybridoma to recombinant production method on 18th September 2023

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Conditional Ambient

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The presenilin 2 (PSEN2) protein also known as AD5 plays a role as a catalytic subunit in the gamma-secretase complex. This complex is involved in intramembrane proteolysis of several type-I transmembrane proteins. PSEN2 has a mass of about 52 kDa and is expressed mainly in the brain and several other tissues throughout the body. Its gene is located on chromosome 1 and has the alternative name 'EP-2614'. Presenilin 2 is closely associated with presenilin 1 (PSEN1) a related protein within the same family.

Biological function summary

Presenilin 2 functions in the regulation of proteolytic processing of the amyloid precursor protein (APP) within the gamma-secretase complex. This action produces amyloid beta peptides which are critical for normal brain function but may accumulate under pathological conditions. The protein is also involved in NOTCH receptor activation which plays a part in cell fate determination during development. The gamma-secretase complex includes other key proteins such as nicastrin anterior pharynx-defective 1 (APH-1) and presenilin enhancer 2 (PEN-2).

Pathways

Presenilin 2 is central to the amyloidogenic pathway through its role in APP processing. This pathway links to Alzheimer's disease where dysregulation of amyloid beta peptide production leads to plaque formation. Additionally presenilin 2 takes part in the NOTCH signaling pathway which influences neurogenesis and cellular differentiation. Presenilin 1 is a partner protein of presenilin 2 within these pathways sharing overlapping functions and regulation.

Presenilin 2's connection to Alzheimer's disease arises from its role in amyloid beta production. Mutations in the PSEN2 gene can lead to familial Alzheimer's disease where altered processing of APP results in neurotoxic peptide accumulation. Additionally presenilin 2 influences cardiovascular disease development through its involvement in cell signaling pathways affecting vascular biology. Presenilin 1 mutations also associate with similar disorders due to its analogous role and participation in these pathological processes.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Catalytic subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (amyloid-beta precursor protein) (PubMed : 10497236, PubMed : 10652302, PubMed : 16752394, PubMed : 27293189, PubMed : 36272978). Selectively cleaves late endosomal/lysosomal localized substrates and generates the prominent pool of intracellular amyloid beta that contains longer amyloid beta (PubMed : 27293189). The holoprotein functions as a calcium-leak channel that allows the passive movement of calcium from endoplasmic reticulum to cytosol and is involved in calcium homeostasis (PubMed : 16959576). Is a regulator of mitochondrion-endoplasmic reticulum membrane tethering and modulates calcium ions shuttling between ER and mitochondria (PubMed : 21285369).

See full target information PSEN2 pS327

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 16:1609340 PubMed40873563

2025

Clonal divergence and genomic meltdown in prostatic pleomorphic giant cell adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoshi Ma,Kun Chen,Jing Zhang,Liming Liu,Jiping Luo,Kaipeng Huang,Hongying Zhang,Danni Liu,Jizhou Gou,Changyin Feng,Xia Zhao,Wanying Li,Lipeng Chen,Li Yin,Xianlin Meng,Zhiqiang Cheng

View all publications

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Abcam Product Promise

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重组Anti-Presenilin 2/AD5 (phospho S327)抗体[EP2613Y]