重组Anti-Prealbumin抗体[EPR20971]

• IHC-Fr

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Immunohistochemistry (Frozen sections) - Anti-Prealbumin antibody [EPR20971] (AB215202)

Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse brain (choroid plexus) tissue labeling Prealbumin with ab215202 at 1/10000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Positive cytoplasmic staining in the endothelial cells of choroid plexus, negative staining in the endothelial cells of blood vessels on mouse brain tissue section (PMID : 19539719; 20462362).

The nuclear counter stain is DAPI (blue).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prealbumin antibody [EPR20971] (AB215202)

Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling Prealbumin with ab215202 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP), Ready to use. Cytoplasmic staining in choroid plexuses (arrows) of mouse cerebrum (PMID : 25758461; PMID : 19032346). Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP), Ready to use.

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-Fr

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Immunohistochemistry (Frozen sections) - Anti-Prealbumin antibody [EPR20971] (AB215202)

Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse liver tissue labeling Prealbumin with ab215202 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Positive cytoplasmic staining in the hepatic cells of mouse liver tissue section (PMID : 25519307).

The nuclear counter stain is DAPI (blue).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prealbumin antibody [EPR20971] (AB215202)

Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling Prealbumin with ab215202 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP), Ready to use. Cytoplasmic staining in mouse liver (PMID : 25519307; PMID : 19032346). Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP), Ready to use.

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IP

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Immunoprecipitation - Anti-Prealbumin antibody [EPR20971] (AB215202)

Prealbumin was immunoprecipitated from 0.35 mg of mouse plasma with ab215202 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab215202 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution

Lane 1 : Mouse plasma 10 μg (Input).

Lane 2 : ab215202 IP in mouse plasma.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab215202 in mouse plasma.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 15 seconds.

The band shown at 30 kDa is likely to be a dimer of Prealbumin based on the literature (PMID : 19279259).

All lanes:

Immunoprecipitation - Anti-Prealbumin antibody [EPR20971] (ab215202)

Predicted band size: 15 kDa

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