重组Anti-PRDM1/Blimp1抗体[EPR25163-80] (ab307644)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR25163-80] to PRDM1/Blimp1
- Suitable for: WB, IHC-P, IP
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-PRDM1/Blimp1抗体[EPR25163-80]
参阅全部 PRDM1/Blimp1 一抗 -
描述
兔单克隆抗体[EPR25163-80] to PRDM1/Blimp1 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, IPmore details
不适用于: Flow Cyt (Intra) or ICC/IF -
种属反应性
与反应: Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: SR lysates IHC-P: Human tonsil tissues. IP: SR cell
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR25163-80 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab307644于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000. Predicted molecular weight: 100 kDa.
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IHC-P |
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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IP |
1/30.
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说明 |
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WB
1/1000. Predicted molecular weight: 100 kDa. |
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
IP
1/30. |
靶标
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功能
Transcriptional repressor that binds specifically to the PRDI element in the promoter of the beta-interferon gene (PubMed:1851123). Drives the maturation of B-lymphocytes into Ig secreting cells (PubMed:12626569). -
序列相似性
Belongs to the class V-like SAM-binding methyltransferase superfamily.
Contains 4 C2H2-type zinc fingers.
Contains 1 SET domain. -
翻译后修饰
Sumoylation at Lys-816 by PIAS1 augments transcriptional repressor activity, and is critical for plasma cell differentiation.
Ubiquitinated by the SCF(FBXO11) complex, leading to its degradation by the proteasome. -
细胞定位
Nucleus. Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 639 Human
- Omim: 603423 Human
- SwissProt: O75626 Human
- Unigene: 436023 Human
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别名
- B Lymphocyte Induced Maturation Protein 1 antibody
- Beta interferon gene positive regulatory domain I binding factor antibody
- Beta-interferon gene positive regulatory domain I-binding factor antibody
see all
图片
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All lanes : Anti-PRDM1/Blimp1 antibody [EPR25163-80] (ab307644) at 1/1000 dilution
Lane 1 : SR (human pleural effusion lymphoblast) whole cell lysate
Lane 2 : Raji (human burkitts lymphoma b lymphocyte) whole cell lysate
Lane 3 : NAMALWA (human burkitts lymphoma b lymphocyte) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution
Predicted band size: 100 kDa
Observed band size: 100 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST
Negative control: Raji, NAMALWA (PMID: 28842558).
Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.
Exposure time: 180 seconds
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Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling PRDM1/Blimp1 with ab307644 at 1/500 (0.924 ug/ml) followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) at Ready to use dilution. Nuclear staining on human tonsil (PMID: 24438193). The section was incubated with ab307644 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection) at Ready to use dilution.
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
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Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling PRDM1/Blimp1 with ab307644 at 1/500 (0.924 ug/ml) followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) at Ready to use dilution. Negative control: No staining on human liver. The section was incubated with ab307644 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection) at Ready to use dilution.
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
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PRDM1/Blimp1 was immunoprecipitated from 0.35 mg SR (human pleural effusion lymphoblast) whole cell lysate 10 µg with ab307644 at 1/30 dilution (2 µg in 0.35 mg lysates). Western blot was performed on the immunoprecipitate using ab307644 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP) (ab131366) was used at 1/5000 dilution.
Lane 1: SR (human pleural effusion lymphoblast) whole cell lysate 10 µg
Lane 2: ab307644 IP in SR whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab307644 in SR whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 50 seconds
Lysate was freshly made and used for Western blotting immediately to minimize protein degradation.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (0)
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