Anti-PRDM16抗体(ab106410)
Key features and details
- Rabbit polyclonal to PRDM16
- Suitable for: ICC, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-PRDM16抗体
参阅全部 PRDM16 一抗 -
描述
兔多克隆抗体to PRDM16 -
宿主
Rabbit -
特异性
At least four isoforms of PRDM16 are known to exist; ab106410 will recognize all four. -
经测试应用
适用于: ICC, IHC-P, WBmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human PRDM16 (C terminal). The immunogen is located within amino acids 1120 - 1170 of PRDM16.
Database link: Q9HAZ2 -
阳性对照
- WB: K562 cell lysate IHC-P: Human brain tissue ICC: Human brain cells
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at +4°C. -
存储溶液
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab106410于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC |
Use a concentration of 20 µg/ml.
|
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| IHC-P | (3) |
Use a concentration of 2 µg/ml.
|
| WB | (3) |
Use a concentration of 1 µg/ml. Predicted molecular weight: 140 kDa.
|
| 说明 |
|---|
|
ICC
Use a concentration of 20 µg/ml. |
|
IHC-P
Use a concentration of 2 µg/ml. |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 140 kDa. |
靶标
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功能
Binds DNA and functions as a transcriptional regulator. Functions in the differentiation of brown adipose tissue (BAT) which is specialized in dissipating chemical energy in the form of heat in response to cold or excess feeding while white adipose tissue (WAT) is specialized in the storage of excess energy and the control of systemic metabolism. Together with CEBPB, regulates the differentiation of myoblastic precursors into brown adipose cells. Functions also as a repressor of TGF-beta signaling. Isoform 4 may regulate granulocytes differentiation. -
组织特异性
Expressed in uterus and kidney. -
疾病相关
Note=A chromosomal aberration involving PRDM16 is found in myelodysplastic syndrome (MDS) and acute myeloid leukemia (AML). Reciprocal translocation t(1;3)(p36;q21). Isoform 4 is specifically expressed in adult T-cell leukemia. -
序列相似性
Contains 10 C2H2-type zinc fingers.
Contains 1 SET domain. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 63976 Human
- Omim: 605557 Human
- SwissProt: Q9HAZ2 Human
- Unigene: 99500 Human
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别名
- CMD1LL antibody
- KIAA1675 antibody
- LVNC8 antibody
see all
图片
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Western blot - Anti-PRDM16 antibody (ab106410)Anti-PRDM16 antibody (ab106410) at 1 µg/ml + K562 (human chronic myelogenous leukemia cell line from bone marrow) cell lysate
Predicted band size: 140 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRDM16 antibody (ab106410)Paraffin-embedded human brain tissue stained for PRDM16 using ab106410 at 2 µg/ml in immunohistochemical analysis.
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Immunocytochemistry - Anti-PRDM16 antibody (ab106410)Image is courtesy of an Abreview submitted by Lena Appelsvedab106410 staining RAP1 in Human Brown adipocytes from supraclavicular fat tissue by ICC (Immunocytochemistry). Cells were fixed with paraformaldehyde, permeabilized with 0.3% Triton X-100 and blocked with 10% serum, 1% BSA for 1 hour at 22°C. Samples were incubated with primary antibody (2 ug/ml in 10% serum, 1% BSA, 0.3% Triton X100, PBS) for 16 hours at 4°C. A Cy3®-conjugated Donkey F(ab')2 anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.
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Immunocytochemistry - Anti-PRDM16 antibody (ab106410)Immunocytochemical analysis of PRDM16 in Human Brain cells using ab106410 at 20 ug/ml.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (79)
ab106410 被引用在 79 文献中.
- Hurwitz E et al. Antagonism between Prdm16 and Smad4 specifies the trajectory and progression of pancreatic cancer. J Cell Biol 222:N/A (2023). PubMed: 36828547
- Wang Z et al. PRDM16 deficiency in vascular smooth muscle cells aggravates abdominal aortic aneurysm. JCI Insight 8:N/A (2023). PubMed: 37079380
- Choi HJ et al. Immunostimulatory and anti‑obesity activities of Adenocaulon himalaicum leaves in RAW264.7 and 3T3‑L1 cells. Exp Ther Med 25:219 (2023). PubMed: 37123212
- Duan G et al. β-Hydroxy-β-methyl Butyrate Regulates the Lipid Metabolism, Mitochondrial Function, and Fat Browning of Adipocytes. Nutrients 15:N/A (2023). PubMed: 37299513
- Choi JW et al. Paeonia lactiflora root decreases lipid accumulation through the induction of lipolysis and thermogenesis via AMPK activation in 3T3‑L1 cells. Int J Mol Med 52:N/A (2023). PubMed: 37326061