AB187881

Anti-PRDM14 抗体

Name: PRDM14抗体 (ab187881)| Abcam中文官网
Brand: Abcam Limited
SKU: AB187881
Price: 4649 CNY
Availability: InStock
Rating: 1 (1 reviews)

Anti-PRDM14 antibody

(1 Review)

(18 Publications)

Rabbit Polyclonal PRDM14 antibody. Suitable for IP, ChIP, WB, IHC-P, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 18 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PR domain zinc finger protein 14.

查看别名

PR domain zinc finger protein 14, PR domain-containing protein 14, PRDM14

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-PRDM14 antibody (AB187881)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-PRDM14 antibody (AB187881)

Immunocytochemistry/Immunofluorescence analysis of A549 cells using ab187881. Blue DAPI for nuclear staining.

ChIP

Supplier Data

ChIP - Anti-PRDM14 antibody (AB187881)

Chromatin immunoprecipitation analysis of RUNX1 gene from 293 cell line, using ab187881 and rabbit IgG. P1, P2, P3 and P4 were four probes located on RUNX1 gene as the schematic diagram illustrated. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Supplier Data

Western blot - Anti-PRDM14 antibody (AB187881)

Western blot analysis of various groups from PA-1 cell line. ShRNA-1, shRNA-2, shRNA-3, shRNA-4, shRNA-5, shRNA-6 show knock-down samples which are transfected by different shRNAs targeting PRDM14. Control represents the untreated one.

All lanes:

Western blot - Anti-PRDM14 antibody (ab187881) at 1/500 dilution

Lane 1:

shRNA-6

Lane 2:

Control

Lane 3:

shRNA-5

Lane 4:

shRNA-4

Lane 5:

shRNA-3

Lane 6:

shRNA-2

Lane 7:

shRNA-1

Predicted band size: 64 kDa

false

Supplier Data

Western blot - Anti-PRDM14 antibody (AB187881)

All lanes:

Western blot - Anti-PRDM14 antibody (ab187881) at 1/500 dilution

Lane 1:

BT474 cell extract

Lane 2:

Jurkat cell extract

Lane 3:

HeLa cell extract

Predicted band size: 64 kDa

false

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Human, Mouse, Rat

应用

ICC/IF, WB, IHC-P, ChIP, IP

applications

免疫原

Recombinant Fragment Protein within Human PR domain zinc finger protein 14. The exact immunogen used to generate this antibody is proprietary information.

Q9GZV8

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ChIP" : {"fullname" : "ChIP", "shortname":"ChIP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "ChIP-species-checked": "testedAndGuaranteed", "ChIP-species-dilution-info": "1/20 - 1/100", "ChIP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

存储溶液

pH: 7.3 Preservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

PRDM14 also known as PR domain zinc finger protein 14 acts as a transcription factor with a mass of approximately 64 kDa. It contains an N-terminal PR (PRDI-BF1 and RIZ) domain and zinc-finger motifs within its structure which participate in DNA binding. The protein expresses highly in embryonic stem cells and primordial germ cells showing a strong presence in pluripotent cell states.

Biological function summary

PRDM14 plays a vital role in the maintenance of pluripotency and regulation of gene expression during early embryonic development. It forms part of a regulatory network that includes other factors like OCT4 and SOX2 promoting stem cell self-renewal and inhibiting differentiation. This protein directly influences the expression of genes related to germ cell formation.

Pathways

PRDM14 interacts with several key signaling pathways notably the stem cell pluripotency pathway and the Wnt signaling pathway. It collaborates closely with proteins such as NANOG and TET proteins modulating gene expression patterns essential for pluripotent stem cell identity. Through these pathways PRDM14 ensures proper cell fate decisions during development.

PRDM14’s dysfunctions associate with certain cancers and reproductive health issues. Aberrant expression or mutation of PRDM14 appear in germ cell tumors as its regulatory functions become disrupted. Additionally PRDM14 may link to infertility disorders due to its role in germ cell development. Alterations in its activity can disrupt interactions with proteins like PRDM1 impacting normal cell proliferation and differentiation.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Transcription factor that has both positive and negative roles on transcription. Required for the maintenance of embryonic stem cell identity and the reacquisition of pluripotency in somatic cells. May play an essential role in germ cell development at 2 levels : the reacquisition of potential pluripotency, including SOX2 up-regulation, and successful epigenetic reprogramming, characterized by EHMT1 repression. Its association with CBFA2T2 is required for the functions in pluripotency and germ cell formation (By similarity). Directly up-regulates the expression of pluripotency gene POU5F1 through its proximal enhancer. Binds to the DNA consensus sequence 5'-GGTC[TC]CTAA-3'.

See full target information PR domain zinc finger protein 14

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文献 (18)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:33308 PubMed41006376

2025

The downregulation of ubiquitin-specific peptidase 2 indicates a poor prognosis and promotes the progression of gastric cancer through focal adhesion and ECM pathway signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Yingjun Liu,Xiao Li,Kena Lian,Youcai Wang,Mingke Huo,Zhi Li,Xiaobin Chen,Jianwei Wang

EMBO reports 25:4950-4978 PubMed39358551

2024

SCAR-6 elncRNA locus epigenetically regulates PROZ and modulates coagulation and vascular function.

Applications

Unspecified application

Species

Unspecified reactive species

Gyan Ranjan,Paras Sehgal,Vinod Scaria,Sridhar Sivasubbu

Molecular cell 84:476-489.e10 PubMed38211589

2024

Pioneer and PRDM transcription factors coordinate bivalent epigenetic states to safeguard cell fate.

Applications

Unspecified application

Species

Unspecified reactive species

Satoshi Matsui,Marissa Granitto,Morgan Buckley,Katie Ludwig,Sandra Koigi,Joseph Shiley,William J Zacharias,Christopher N Mayhew,Hee-Woong Lim,Makiko Iwafuchi

International journal of molecular sciences 24: PubMed37569280

2023

Mixed-Lineage Leukemia 1 Inhibition Enhances the Differentiation Potential of Bovine Embryonic Stem Cells by Increasing H3K4 Mono-Methylation at Active Promoters.

Applications

Unspecified application

Species

Unspecified reactive species

Chen Li,Xuejie Han,Jing Wang,Fang Liu,Yuanyuan Zhang,Zihong Li,Zhenyu Lu,Yongli Yue,Jinzhu Xiang,Xueling Li

Stem cell investigation 10:10 PubMed37155477

2023

Transcriptomic analysis of feeder-free culture system for maintaining naïve-state pluripotency in human pluripotent stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Wataru Isono,Tomoyuki Kawasaki,Justin K Ichida,Kazunori Nagasaka,Osamu Hiraike,Akihiro Umezawa,Hidenori Akutsu

American journal of cancer research 13:1471-1485 PubMed37168326

2023

Somatic-to-primordial germ cell-like transformation is critical in tumor initiation of mouse breast tumor 4T1 cells.

Applications

Unspecified application

Species

Unspecified reactive species

Zhan Ma,Fengyu Zhang,Aiping Liu,Huikuan Lin,Chunfang Liu

Science advances 8:eabk0013 PubMed35333572

2022

Genome-wide screening identifies Polycomb repressive complex 1.3 as an essential regulator of human naïve pluripotent cell reprogramming.

Applications

Unspecified application

Species

Unspecified reactive species

Amanda J Collier,Adam Bendall,Charlene Fabian,Andrew A Malcolm,Katarzyna Tilgner,Claudia I Semprich,Katarzyna Wojdyla,Paola Serena Nisi,Kamal Kishore,Valar Nila Roamio Franklin,Bahar Mirshekar-Syahkal,Clive D'Santos,Kathrin Plath,Kosuke Yusa,Peter J Rugg-Gunn

International journal of cancer 149:646-656 PubMed33783816

2021

Treatment of primary and metastatic breast and pancreatic tumors upon intravenous delivery of a PRDM14-specific chimeric siRNA/nanocarrier complex.

Applications

Unspecified application

Species

Unspecified reactive species

Hiroaki Taniguchi,Yukikazu Natori,Yohei Miyagi,Kotaro Hayashi,Fumitaka Nagamura,Kazunori Kataoka,Kohzoh Imai

Molecular medicine reports 23: PubMed33355367

2020

PRDM14 mediates chemosensitivity and glycolysis in drug‑resistant A549/cisplatin cells and their progenitor A549 human lung adenocarcinoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Saifei He,Xiaokun Ma,Ni Zheng,Guoyu Wang,Menghan Wang,Wei Xia,Donghai Yu

Regenerative therapy 15:161-168 PubMed33426214

2020

The combination of dibenzazepine and a DOT1L inhibitor enables a stable maintenance of human naïve-state pluripotency in non-hypoxic conditions.

Applications

Unspecified application

Species

Unspecified reactive species

Wataru Isono,Tomoyuki Kawasaki,Justin K Ichida,Takuya Ayabe,Osamu Hiraike,Akihiro Umezawa,Hidenori Akutsu

View all publications

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Anti-PRDM14 抗体

Anti-PRDM14 antibody

Immunocytochemistry/ Immunofluorescence - Anti-PRDM14 antibody (AB187881)

ChIP - Anti-PRDM14 antibody (AB187881)

Western blot - Anti-PRDM14 antibody (AB187881)

Western blot - Anti-PRDM14 antibody (AB187881)