AB197890

重组Anti-PRD抗体[EPR16959]

Anti-PRD antibody [EPR16959]

RabMAb
Recombinant
了解详情

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(4 Publications)

Rabbit Recombinant Monoclonal PRD antibody. Suitable for WB and reacts with Human samples. Cited in 4 publications.

查看别名

PRD, PEPD, Xaa-Pro dipeptidase, X-Pro dipeptidase, Imidodipeptidase, Peptidase D, Proline dipeptidase, Prolidase

2 Images

Supplier Data

Western blot - Anti-PRD antibody [EPR16959] (AB197890)

Blocking and dilution buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-PRD antibody [EPR16959] (ab197890) at 1/10000 dilution

Lane 1:

HepG2 (Human liver hepatocellular carcinoma) whole cell lysate at 20 µg

Lane 2:

SH-SY5Y (Human neuroblastoma from bone marrow cells) whole cell lysate at 20 µg

Lane 3:

293 (Human epithelial cells from embryonic kidney) whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 55 kDa

false

Supplier Data

Western blot - Anti-PRD antibody [EPR16959] (AB197890)

Blocking/dilution buffer : 5% NFDM /TBST

All lanes:

Western blot - Anti-PRD antibody [EPR16959] (ab197890) at 1/1000 dilution

Lane 1:

Human fetal kidney lysate at 10 µg

Lane 2:

Human fetal placenta lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 55 kDa

false

不同偶联物与剂型 (1)

Carrier free

Anti-PRD antibody [EPR16959] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR16959

亚型

IgG

不含载体蛋白

反应种属

Human

应用

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Conditional Ambient

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

PRD also known as the podocyte retinoid-dependent domain is a protein of interest in several research areas. This protein has a known mass of approximately 62 kDa. PRD expresses in kidney podocytes specialized cells responsible for filtration in the renal glomerulus. The protein plays an important role mechanically in maintaining podocyte structure and function contributing to the integrity of the glomerular filtration barrier.

Biological function summary

PRD interacts with cellular mechanisms critical for podocyte stability. PRD associates with lipid rafts specialized membrane structures suggesting a role in signal transduction. It functions as part of a larger protein complex that includes nephrin and podocin essential components of the slit diaphragm in podocytes. PRD is involved in cytoskeletal dynamics regulating actin filament organization important for maintaining cell shape and adhesion.

Pathways

Several cellular activities incorporate PRD into their processes. It is closely tied to the PI3K/AKT signaling pathway a critical pathway regulating cell survival and growth. In the slit diaphragm context PRD works alongside nephrin and p130Cas forming a signaling cascade that ensures effective communication between podocytes. These pathways help maintain the health of the glomerular filtration system by responding to mechanical and biochemical stimuli.

The disruption of PRD function links to renal diseases such as nephrotic syndrome and focal segmental glomerulosclerosis (FSGS). Mutations or alterations in PRD expression or interaction can impair the slit diaphragm leading to proteinuria and progressive kidney damage. The protein interacts with nephrin and podocin whose mutations are also implicated in these disorders highlighting its importance in preserving kidney function.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Dipeptidase that catalyzes the hydrolysis of dipeptides with a prolyl (Xaa-Pro) or hydroxyprolyl residue in the C-terminal position (PubMed : 17081196, PubMed : 35165443). The preferred dipeptide substrate is Gly-Pro, but other Xaa-Pro dipeptides, such as Ala-Pro, Met-Pro, Phe-Pro, Val-Pro and Leu-Pro, can be cleaved (PubMed : 17081196). Plays an important role in collagen metabolism because the high level of iminoacids in collagen (PubMed : 2925654).

See full target information PEPD

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文献 (4)

Recent publications for all applications. Explore the full list and refine your search

Aging 16:10997-11017 PubMed38968580

2024

Pan-cancer analysis of disulfidptosis with potential implications in prognosis, immune microenvironment, and drug resistance in human cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Fobao Lai,Wanrong Zheng,Chengqian Zhong,Zhiyong Chen

Journal of medicinal chemistry 66:2589-2607 PubMed36724486

2023

Optimized M24B Aminopeptidase Inhibitors for CARD8 Inflammasome Activation.

Applications

Unspecified application

Species

Unspecified reactive species

Qifeng Chen,Alvin Wang,Dominic J Covelli,Abir Bhattacharjee,Qinghui Wang,Elizabeth L Orth-He,Sahana D Rao,Hsin-Che Huang,Daniel P Ball,Jeffrey C Hsiao,Daniel A Bachovchin

Nature chemical biology 18:565-574 PubMed35165443

2022

M24B aminopeptidase inhibitors selectively activate the CARD8 inflammasome.

Applications

Unspecified application

Species

Unspecified reactive species

Sahana D Rao,Qifeng Chen,Qinghui Wang,Elizabeth L Orth-He,Michelle Saoi,Andrew R Griswold,Abir Bhattacharjee,Daniel P Ball,Hsin-Che Huang,Ashley J Chui,Dominic J Covelli,Shaochen You,Justin R Cross,Daniel A Bachovchin

Communications biology 4:1373 PubMed34880421

2021

Loss of peptidase D binding restores the tumor suppressor functions of oncogenic p53 mutants.

Applications

Unspecified application

Species

Unspecified reactive species

Lu Yang,Yun Li,Arup Bhattacharya,Yuesheng Zhang

View all publications

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Abcam Product Promise

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重组Anti-PRD抗体[EPR16959]