重组Anti-PRC1 (phospho T481)抗体[EP1514Y] (ab62366)
![Dot Blot - Anti-PRC1 (phospho T481) antibody [EP1514Y] (ab62366)](https://www.abcam.cn/ps/products/62/ab62366/Images/ab62366-280631-ab62366dot-002.jpg "Dot Blot - Anti-PRC1 (phospho T481) antibody [EP1514Y] (ab62366)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1514Y] to PRC1 (phospho T481)
- Suitable for: WB, Dot blot
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-PRC1 (phospho T481)抗体[EP1514Y]
参阅全部 PRC1 一抗 -
描述
兔单克隆抗体[EP1514Y] to PRC1 (phospho T481) -
宿主
Rabbit -
经测试应用
适用于: WB, Dot blotmore details
不适用于: Flow Cyt or IP -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- HeLa cells and HeLa whole cell lysate (ab150035).
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP1514Y -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab62366于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500. Detects a band of approximately 72 kDa (predicted molecular weight: 72 kDa).
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| Dot blot |
Use at an assay dependent concentration.
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| 说明 |
|---|
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WB
1/500. Detects a band of approximately 72 kDa (predicted molecular weight: 72 kDa). |
|
Dot blot
Use at an assay dependent concentration. |
应用说明
Is unsuitable for Flow Cyt or IP.
靶标
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功能
Cross-links antiparrallel microtubules at an average distance of 35 nM. Essential for controlling the spatiotemporal formation of the midzone and successful cytokinesis. Required for KIF14 localization to the central spindle and midbody. -
序列相似性
Belongs to the MAP65/ASE1 family. -
结构域
Microtubule binding occurs via a basic patch in the central spectrin-like domain and requires also the unstructured C-terminal domain. -
翻译后修饰
Phosphorylation by CDK1 in early mitosis holds PRC1 in an inactive monomeric state, during the metaphase to anaphase transition, PRC1 is dephosphorylated, promoting interaction with KIF4A, which then translocates PRC1 along mitotic spindles to the plus ends of antiparallel interdigitating microtubules. Dephosphorylation also promotes MT-bundling activity by allowing dimerization. -
细胞定位
Nucleus. Cytoplasm. Cytoplasm > cytoskeleton > spindle pole. Predominantly localized to the nucleus of interphase cells. During mitosis becomes associated with the mitotic spindle poles and localizes with the cell midbody during cytokinesis. - Information by UniProt
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数据库链接
- Entrez Gene: 9055 Human
- Omim: 603484 Human
- SwissProt: O43663 Human
- Unigene: 366401 Human
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别名
- Anaphase spindle elongation 1 homolog antibody
- ASE1 antibody
- PRC1 antibody
see all
图片
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Dot Blot - Anti-PRC1 (phospho T481) antibody [EP1514Y] (ab62366)
Primary ab dilution 1:1000 dilution. Secondary ab description and code (ab id) Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051). Secondary ab dilution 1:100,000 dilution. Blocking buffer and concentration 5% NFDM/TBST. Lane 1: PRC1 (pT481) phospho peptide Lane 2: PRC1 non-phospho peptide. Exposure time 3 minutes
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![Western blot - Anti-PRC1 (phospho T481) antibody [EP1514Y] (ab62366)](https://www.abcam.cn/ps/products/62/ab62366/Images/ab62366-75694-ab62366-WB-002.jpg)
Western blot - Anti-PRC1 (phospho T481) antibody [EP1514Y] (ab62366)All lanes : Anti-PRC1 (phospho T481) antibody [EP1514Y] (ab62366) at 1/500 dilution
Lane 1 : HeLa cell lysates (untreated)
Lane 2 : HeLa cell lysates treated with aphidicolin + nocodazole
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : goat anti-rabbit HRP labeled, at 1/2000 dilution
Predicted band size: 72 kDa
Observed band size: 72 kDa -
![Anti-PRC1 (phospho T481) antibody [EP1514Y] (ab62366)](https://www.abcam.cn/ps/products/62/ab62366/Images/ab62366-5-benefits-of-recombinant-antibodies.png)
Anti-PRC1 (phospho T481) antibody [EP1514Y] (ab62366)
数据表及文件
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SDS download
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Datasheet download
文献 (8)
ab62366 被引用在 8 文献中.
- Sobajima T et al. PP6 regulation of Aurora A-TPX2 limits NDC80 phosphorylation and mitotic spindle size. J Cell Biol 222:N/A (2023). PubMed: 36897279
- Galarreta A et al. USP7 limits CDK1 activity throughout the cell cycle. EMBO J 40:e99692 (2021). PubMed: 33856059
- Prokhorova E et al. Unrestrained poly-ADP-ribosylation provides insights into chromatin regulation and human disease. Mol Cell 81:2640-2655.e8 (2021). PubMed: 34019811
- Serena M et al. Molecular basis of MKLP2-dependent Aurora B transport from chromatin to the anaphase central spindle. J Cell Biol 219:N/A (2020). PubMed: 32356865
- Holder J et al. Ordered dephosphorylation initiated by the selective proteolysis of cyclin B drives mitotic exit. Elife 9:N/A (2020). PubMed: 32869743
- Capalbo L et al. The midbody interactome reveals unexpected roles for PP1 phosphatases in cytokinesis. Nat Commun 10:4513 (2019). PubMed: 31586073
- Bartlett E et al. Interplay of Histone Marks with Serine ADP-Ribosylation. Cell Rep 24:3488-3502.e5 (2018). PubMed: 30257210
- Cundell MJ et al. A PP2A-B55 recognition signal controls substrate dephosphorylation kinetics during mitotic exit. J Cell Biol 214:539-54 (2016). PubMed: 27551054