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Anti-PRC1抗体[16F2] (ab119338)

Submit a review Submit a question References (2)
Western blot - Anti-PRC1 antibody [16F2] (ab119338)
  • Immunocytochemistry/ Immunofluorescence - Anti-PRC1 antibody [16F2] (ab119338)
  • Immunocytochemistry/ Immunofluorescence - Anti-PRC1 antibody [16F2] (ab119338)
  • Immunocytochemistry/ Immunofluorescence - Anti-PRC1 antibody [16F2] (ab119338)
  • Immunocytochemistry/ Immunofluorescence - Anti-PRC1 antibody [16F2] (ab119338)

Key features and details

  • Mouse monoclonal [16F2] to PRC1
  • Suitable for: ICC/IF, WB
  • Reacts with: Human
  • Isotype: IgG2b

选择批间可重复性更高的重组抗体

Product image
Anti-PRC1 antibody [EP1513Y] (ab51248)
  • 研究可靠 —— 各批次间结果一致且可重复
  • 长期批量供应 —— 采用重组技术，可实现快速生产
  • 首次实验即可成功 —— 经过大量验证确认了特异性
  • 符合伦理标准 —— 产品不含动物成分

概述

  • 产品名称

    Anti-PRC1抗体[16F2]
    参阅全部 PRC1 一抗

  • 描述

    小鼠单克隆抗体[16F2] to PRC1

  • 宿主

    Mouse

  • 经测试应用

    适用于: ICC/IF, WBmore details

  • 种属反应性

    与反应: Human

  • 免疫原

    Recombinant Human PRC1

  • 阳性对照

    • WB: PANC-1, HCT 116, C2C12, HeLa, SH-SY5Y, K-562, Raji whole cell extracts; ICC/IF: HeLa, A2058, U251 cells

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • 存储溶液

    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...

  • 纯度

    Protein G purified

  • 克隆

    单克隆

  • 克隆编号

    16F2

  • 同种型

    IgG2b

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab119338于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC/IF
1/100.
WB
Use a concentration of 3 µg/ml. Predicted molecular weight: 72 kDa.
说明
ICC/IF
1/100.
WB
Use a concentration of 3 µg/ml. Predicted molecular weight: 72 kDa.

靶标

  • 功能

    Cross-links antiparrallel microtubules at an average distance of 35 nM. Essential for controlling the spatiotemporal formation of the midzone and successful cytokinesis. Required for KIF14 localization to the central spindle and midbody.

  • 序列相似性

    Belongs to the MAP65/ASE1 family.

  • 结构域

    Microtubule binding occurs via a basic patch in the central spectrin-like domain and requires also the unstructured C-terminal domain.

  • 翻译后修饰

    Phosphorylation by CDK1 in early mitosis holds PRC1 in an inactive monomeric state, during the metaphase to anaphase transition, PRC1 is dephosphorylated, promoting interaction with KIF4A, which then translocates PRC1 along mitotic spindles to the plus ends of antiparallel interdigitating microtubules. Dephosphorylation also promotes MT-bundling activity by allowing dimerization.

  • 细胞定位

    Nucleus. Cytoplasm. Cytoplasm > cytoskeleton > spindle pole. Predominantly localized to the nucleus of interphase cells. During mitosis becomes associated with the mitotic spindle poles and localizes with the cell midbody during cytokinesis.
  • Information by UniProt

  • 数据库链接

    • 别名

      • Anaphase spindle elongation 1 homolog antibody
      • ASE1 antibody
      • PRC1 antibody
      • PRC1_HUMAN antibody
      • Protein regulating cytokinesis 1 antibody
      • Protein regulator of cytokinesis 1 antibody
      see all

    图片

    • Western blot - Anti-PRC1 antibody [16F2] (ab119338)
      Western blot - Anti-PRC1 antibody [16F2] (ab119338)
      All lanes : Anti-PRC1 antibody [16F2] (ab119338) at 3 µg/ml

      Lane 1 : PANC-1 (human pancreatic epithelial carcinoma cell line) whole cell extract
      Lane 2 : HCT 116 (human colorectal carcinoma cell line) whole cell extract
      Lane 3 : C2C12 (mouse myoblast cell line) whole cell extract
      Lane 4 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
      Lane 5 : SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell extract
      Lane 6 : K-562 (human chronic myelogenous leukemia cell line from bone marrow ) whole cell extract
      Lane 7 : Raji (human Burkitt's lymphoma cell line) whole cell extract

      Lysates/proteins at 30 µg per lane.

      Secondary
      All lanes : Goat anti-Mouse IgG (H+L) Secondary Antibody, HRP conjugate at 1/4000 dilution

      Predicted band size: 72 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-PRC1 antibody [16F2] (ab119338)
      Immunocytochemistry/ Immunofluorescence - Anti-PRC1 antibody [16F2] (ab119338)

      Immunocytochemistry analysis of PRC1 using ab119338 at 2µg/mL concentration shows staining in 4% paraformaldehyde-fixed 0.1% Triton X-100 permeabilized HeLa Cells. PRC1 (green), F-Actin staining with Alexa Fluor® 555 Rhodamine Phalloidin (red) and nuclei with SlowFade® Gold Antifade Mountant with DAPI (blue) is shown. Negative control has no primary antibody.

    • Immunocytochemistry/ Immunofluorescence - Anti-PRC1 antibody [16F2] (ab119338)
      Immunocytochemistry/ Immunofluorescence - Anti-PRC1 antibody [16F2] (ab119338)

      Immunofluorescent analysis of PRC1 using PRC1 Monoclonal Antibody (16F2) (ab119338) shows staining in U251 Cells. PRC1 (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing PRC1 (ab119338) at a dilution of 1:20 over night at 4oC, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.

    • Immunocytochemistry/ Immunofluorescence - Anti-PRC1 antibody [16F2] (ab119338)
      Immunocytochemistry/ Immunofluorescence - Anti-PRC1 antibody [16F2] (ab119338)

      Immunofluorescent analysis of PRC1 using PRC1 Monoclonal Antibody (16F2) (ab119338) shows staining in Hela Cells. PRC1 (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing PRC1 (ab119338) at a dilution of 1:100 over night at 4oC, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.

    • Immunocytochemistry/ Immunofluorescence - Anti-PRC1 antibody [16F2] (ab119338)
      Immunocytochemistry/ Immunofluorescence - Anti-PRC1 antibody [16F2] (ab119338)

      Immunofluorescent analysis of PRC1 using PRC1 Monoclonal Antibody (16F2) (ab119338 ) shows staining in A2058 Cells. PRC1 (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing PRC1 (ab119338 ) at a dilution of 1:100 over night at 4 oC, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.

    文献 (2)

    发表研究结果有使用 ab119338？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab119338 被引用在 2 文献中.

    • Wang Q  et al. Analysis of transcriptome in the relationship between expression of PRC1 protein and prognosis of patients with cholangiocarcinoma. J Int Med Res 49:300060521989200 (2021). PubMed: 33706578
    • Moawia A  et al. Mutations of KIF14 cause primary microcephaly by impairing cytokinesis. Ann Neurol 82:562-577 (2017). PubMed: 28892560

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