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AB151092

Anti-PRAM1抗体

Anti-PRAM1 antibody

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(1 Publication)

Rabbit Polyclonal PRAM1 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human PML-RARA-regulated adapter molecule 1 aa 150-250.

查看别名

PML-RARA-regulated adapter molecule 1, PRAM, PRAM-1, PRAM1

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRAM1 antibody (AB151092)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRAM1 antibody (AB151092)

Immunohistochemical analysis of paraffin-embedded Human bone marrow tissue labeling PRAM1 with ab151092 at 1/500 dilution.

Western blot - Anti-PRAM1 antibody (AB151092)
  • WB

Unknown

Western blot - Anti-PRAM1 antibody (AB151092)

All lanes:

Western blot - Anti-PRAM1 antibody (ab151092) at 1/250 dilution

Lane 1:

RT-4 lysate

Lane 2:

U-251 MG lysate

Lane 3:

Human plasma lysate

Lane 4:

Human liver lysate

Lane 5:

Human tonsil lysate

Predicted band size: 79 kDa

false

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

No

反应种属

Human

应用

WB, IHC-P

applications

免疫原

Recombinant Fragment Protein within Human PML-RARA-regulated adapter molecule 1 aa 150-250. The exact immunogen used to generate this antibody is proprietary information.

Q96QH2

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/250 - 1/500", "WB-species-notes": "<p></p>" } } }
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产品详情

The antibody solution should be gently mixed before use.
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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Immunogen
存储溶液
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

PRAM1 also known as PML-associated protein is an adaptor protein with a mass of approximately 101 kDa. The protein predominantly localizes in hematopoietic cells such as myeloid and lymphoid cells. PRAM1 plays an integral role in signal transduction processes by acting as a scaffolding protein. It possesses several domains facilitating interactions with other signaling molecules contributing to cellular communication and response.
Biological function summary

PRAM1 influences immune cell function by modulating intracellular signaling pathways. It does not work alone as it is part of larger signaling complexes interacting with other proteins to transmit extracellular signals into cellular responses. Through its adaptor role PRAM1 influences cellular processes like differentiation proliferation and apoptosis specifically in immune cells. This involvement highlights its importance in maintaining immune system balance and response to external stimuli.

Pathways

The PRAM1 protein participates in key signaling networks like the MAPK and NF-kB pathways. In these pathways PRAM1 interacts with proteins such as SOS and MEKK1 representing important nodes in the cascade. The MAPK pathway plays a significant role in cellular responses to stress and mitogenic signals while the NF-kB pathway is central to immune response regulation and inflammatory processes. PRAM1's participation in these critical pathways reflects its broader impact on cellular signaling and function.

Abnormalities in PRAM1 expression or function may contribute to leukemias and autoimmune conditions. For instance PRAM1 deregulation has links to acute promyelocytic leukemia (APL) where its interaction with the PML-RAR fusion protein influences the disease's pathophysiology. PRAM1 also relates to systemic lupus erythematosus potentially affecting disease progression through alterations in signal transduction pathways and immune cell function. Understanding PRAM1's role in these disorders highlights its potential as a therapeutic target in hematological malignancies and autoimmune disease management.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

May be involved in myeloid differentiation. May be involved in integrin signaling in neutrophils. Binds to PtdIns(4)P.
See full target information PML-RARA-regulated adapter molecule 1
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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 14:1268141 PubMed38035073

2023

A comprehensive cuproptosis score and associated gene signatures reveal prognostic and immunological features of idiopathic pulmonary fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Chuanqing Jing,Rong Fu,Xue Liu,Guodong Zang,Xue Zhu,Can Wang,Wei Zhang
View all publications
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