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AB91181

Anti-PR3 抗体 [WGM2]

Anti-PR3 antibody [WGM2]

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(13 Publications)

Mouse Monoclonal PR3 antibody. Suitable for Flow Cyt, ELISA, WB, IHC-P, FuncS, Inhib, IHC-Fr and reacts with Human samples. Cited in 13 publications.

查看别名

MBN, PRTN3, Myeloblastin, AGP7, C-ANCA antigen, Leukocyte proteinase 3, Neutrophil proteinase 4, P29, Wegener autoantigen, PR-3, PR3, NP-4

不同偶联物与剂型 (1)

  • 519 FITC

    FITC Anti-PR3 antibody [WGM2]

关键信息

宿主种属

Mouse

克隆

Monoclonal

克隆号

WGM2

亚型

IgG1

不含载体蛋白

No

反应种属

Human

应用

FuncS, ELISA, WB, IHC-Fr, IHC-P, Flow Cyt, Inhib

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FuncS" : {"fullname" : "Functional Studies", "shortname":"FuncS"}, "Inhib" : {"fullname" : "Inhibition Assay", "shortname":"Inhib"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p><a href='/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "FuncS-species-checked": "guaranteed", "FuncS-species-dilution-info": "", "FuncS-species-notes": "<p></p>", "Inhib-species-checked": "guaranteed", "Inhib-species-dilution-info": "", "Inhib-species-notes": "<p></p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" } } }

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为什么推荐这个?

我们推荐这个产品,因为它经常被用于相同的实验或相关的研究。

我们建议您务必查阅产品说明书,以确认其是否符合您的实验需求;若有疑问,也可联系我们的技术团队获取协助。

产品详情

ab91181 blocks the PR3 activity and partially inhibits the binding of Human PR3-ANCA to PR3; it can be used in immuno assays, for inhibition of biological PR3 activity and induction of monocytes and neutrophils activation.

性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein G
纯化说明
0.2 µm filtered antibody solution
存储溶液
Constituents: PBS, 0.1% BSA
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Proteinase 3 (PR3) also known as PRTN3 is a serine protease with a mass of approximately 29 kDa. It belongs to the human neutrophil elastase (HNE) family and is mainly expressed in neutrophils. Within these immune cells PR3 localizes to azurophilic granules where it participates in early immune responses. The proteolytic activity of PR3 involves cleaving peptide bonds in target proteins contributing to the processing and degradation of various proteins important for immune function.
Biological function summary

Proteinase 3 serves multiple roles including modulating inflammation and immune responses. PR3 functions as part of a complex with azurophilic granules aiding in microbial defense mechanisms. In addition the protease regulates cytokine activation and deactivation which in turn influences cell signaling related to inflammation. Its enzymatic action ensures that PR3 can manage inflammatory processes by breaking down extracellular matrix components thereby allowing white blood cells to access infection sites.

Pathways

Proteinase 3 plays an important role within the serine protease and inflammatory pathways. It interacts with other proteins such as neutrophil elastase with which it shares a pathway to modulate immune responses. PR3 also partners with cathepsin G contributing to processes such as pathogen clearance and tissue remodeling providing a coordinated response during infection and injury. These interactions show PR3's involvement in regulating the balance between inflammation and tissue repair.

PR3 is notably associated with Wegener's granulomatosis also known as granulomatosis with polyangiitis. This autoimmune condition results in systemic vasculitis where anti-proteinase 3 antibodies notably characterize the disease. Additionally PR3 plays a part in chronic obstructive pulmonary disease (COPD) where its proteolytic activity contributes to tissue damage in the lungs. In both conditions PR3 interplays with related proteins like myeloperoxidase further demonstrating its importance in processes leading to these disorders.

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

靶点信息

Serine protease that degrades elastin, fibronectin, laminin, vitronectin, and collagen types I, III, and IV (in vitro) (PubMed : 2033050, PubMed : 28240246, PubMed : 3198760). By cleaving and activating receptor F2RL1/PAR-2, enhances endothelial cell barrier function and thus vascular integrity during neutrophil transendothelial migration (PubMed : 23202369). May play a role in neutrophil transendothelial migration, probably when associated with CD177 (PubMed : 22266279).
See full target information PRTN3

文献 (13)

Recent publications for all applications. Explore the full list and refine your search

Oncoimmunology 11:2052418 PubMed35309732

2022

Neutrophil extracellular traps induce tumor metastasis through dual effects on cancer and endothelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Ze-Zhou Jiang,Zhi-Peng Peng,Xing-Chen Liu,Hao-Fan Guo,Meng-Meng Zhou,Da Jiang,Wan-Ru Ning,Yu-Fan Huang,Limin Zheng,Yan Wu

BMC immunology 20:22 PubMed31248381

2019

Macrophage migration inhibitory factor is involved in antineutrophil cytoplasmic antibody-mediated activation of C5a-primed neutrophils.

Applications

Unspecified application

Species

Unspecified reactive species

Jian Hao,Tiegang Lv,Liping Xu,Mao Ran,Kaili Wu

Journal of the American Chemical Society 139:10115-10125 PubMed28672107

2017

Toolbox of Fluorescent Probes for Parallel Imaging Reveals Uneven Location of Serine Proteases in Neutrophils.

Applications

Unspecified application

Species

Unspecified reactive species

Paulina Kasperkiewicz,Yoav Altman,Maximiliano D'Angelo,Guy S Salvesen,Marcin Drag

Disease models & mechanisms 9:885-97 PubMed27483354

2016

Alterations in nuclear structure promote lupus autoimmunity in a mouse model.

Applications

Unspecified application

Species

Human

Namrata Singh,Duncan B Johnstone,Kayla A Martin,Italo Tempera,Mariana J Kaplan,Michael F Denny

PloS one 9:e110677 PubMed25330301

2014

Vesicular LL-37 contributes to inflammation of the lesional skin of palmoplantar pustulosis.

Applications

Unspecified application

Species

Unspecified reactive species

Masamoto Murakami,Takaaki Kaneko,Teruaki Nakatsuji,Kenji Kameda,Hidenori Okazaki,Xiuju Dai,Yasushi Hanakawa,Mikiko Tohyama,Akemi Ishida-Yamamoto,Koji Sayama

Arthritis research & therapy 16:R142 PubMed25000985

2014

The interaction between C5a and sphingosine-1-phosphate in neutrophils for antineutrophil cytoplasmic antibody mediated activation.

Applications

Unspecified application

Species

Unspecified reactive species

Jian Hao,Yi-Min Huang,Ming-Hui Zhao,Min Chen

Innate immunity 20:440-8 PubMed24189124

2013

Myeloperoxidase influences the complement regulatory function of modified C-reactive protein.

Applications

Unspecified application

Species

Human

Peng-cheng Xu,Zhi-ying Li,Xiao-wei Yang,Ming-hui Zhao,Min Chen

Journal of immunology (Baltimore, Md. : 1950) 189:5476-84 PubMed23105141

2012

Broad cross-presentation of the hematopoietically derived PR1 antigen on solid tumors leads to susceptibility to PR1-targeted immunotherapy.

Applications

Unspecified application

Species

Unspecified reactive species

Gheath Alatrash,Elizabeth A Mittendorf,Anna Sergeeva,Pariya Sukhumalchandra,Na Qiao,Mao Zhang,Lisa S St John,Kathryn Ruisaard,Christine E Haugen,Zein Al-Atrache,Haroon Jakher,Anne V Philips,Xiaoling Ding,Jie Qing Chen,Yun Wu,Rebecca S Patenia,Chantale Bernatchez,Luis M Vence,Laszlo G Radvanyi,Patrick Hwu,Karen Clise-Dwyer,Qing Ma,Sijie Lu,Jeffrey J Molldrem

PloS one 7:e38317 PubMed22675451

2012

p38MAPK, ERK and PI3K signaling pathways are involved in C5a-primed neutrophils for ANCA-mediated activation.

Applications

Flow Cyt

Species

Human

Jian Hao,Li-Qiang Meng,Peng-Cheng Xu,Min Chen,Ming-Hui Zhao

Rheumatology (Oxford, England) 41:1313-7 PubMed12422006

2002

A critical evaluation of commercial immunoassays for antineutrophil cytoplasmic antibodies directed against proteinase 3 and myeloperoxidase in Wegener's granulomatosis and microscopic polyangiitis.

Applications

Unspecified application

Species

Unspecified reactive species

E Csernok,D Ahlquist,S Ullrich,W L Gross
View all publications

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