Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online

Anti-PPTC7抗体(ab122548)

Submit a review Q&A (1)References (1)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPTC7 antibody (ab122548)
  • Western blot - Anti-PPTC7 antibody (ab122548)
  • Immunocytochemistry/ Immunofluorescence - Anti-PPTC7 antibody (ab122548)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPTC7 antibody (ab122548)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPTC7 antibody (ab122548)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPTC7 antibody (ab122548)
  • Western blot - Anti-PPTC7 antibody (ab122548)

Key features and details

  • Rabbit polyclonal to PPTC7
  • Suitable for: IHC-P, WB, ICC/IF
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

概述

  • 产品名称

    Anti-PPTC7抗体

  • 描述

    兔多克隆抗体to PPTC7

  • 宿主

    Rabbit

  • 经测试应用

    适用于: IHC-P, WB, ICC/IFmore details

  • 种属反应性

    与反应: Mouse, Rat, Human

  • 免疫原

    amino acids 118-215 (ILTTSYCELL QNKVPLLGSS TACIVVLDRT SHRLHTANLG DSGFLVVRGG EVVHRSDEQQ HYFNTPFQLS IAPPEAEGVV LSDSPDAADS TSFDVQL) of the Human PPTC7 protein (Q8NI37).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • 阳性对照

    • IHC-P: Human testis, rectum, liver and kidney tissue. WB: RT-4, U-251 MG, NIH-3T3, NBT-II. ICC/IF: A-431 cells.

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • 存储溶液

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)
  • Concentration information loading...

  • 纯度

    Immunogen affinity purified

  • 克隆

    多克隆

  • 同种型

    IgG

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab122548于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
Use a concentration of 0.04 - 0.4 µg/ml.
ICC/IF
Use a concentration of 0.25 - 2 µg/ml.
说明
IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
Use a concentration of 0.04 - 0.4 µg/ml.
ICC/IF
Use a concentration of 0.25 - 2 µg/ml.

靶标

图片

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPTC7 antibody (ab122548)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPTC7 antibody (ab122548)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded human testis tissue labeling PPTC7 with ab122548 at a 1/500 dilution.

  • Western blot - Anti-PPTC7 antibody (ab122548)
    Western blot - Anti-PPTC7 antibody (ab122548)
    All lanes : Anti-PPTC7 antibody (ab122548) at 0.4 µg/ml

    Lane 1 : RT4 Cell lysate
    Lane 2 : U 251 MG Cell lysate
  • Immunocytochemistry/ Immunofluorescence - Anti-PPTC7 antibody (ab122548)
    Immunocytochemistry/ Immunofluorescence - Anti-PPTC7 antibody (ab122548)
    Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli, plasma membrane and cytoplasm. Recommended concentration of ab122548 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPTC7 antibody (ab122548)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPTC7 antibody (ab122548)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded human rectum tissue labeling PPTC7 with ab122548 at a 1/500 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPTC7 antibody (ab122548)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPTC7 antibody (ab122548)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded human kidney tissue labeling PPTC7 with ab122548 at a 1/500 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPTC7 antibody (ab122548)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPTC7 antibody (ab122548)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded human liver tissue labeling PPTC7 with ab122548 at a 1/500 dilution.

  • Western blot - Anti-PPTC7 antibody (ab122548)
    Western blot - Anti-PPTC7 antibody (ab122548)
    All lanes : Anti-PPTC7 antibody (ab122548) at 0.4 µg/ml

    Lane 1 : NIH-3T3 cell lysate
    Lane 2 : NBT-II cell lysate

文献 (1)

发表研究结果有使用 ab122548？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab122548 被引用在 1 文献中.

  • Mishra A  et al. A protein phosphatase network controls the temporal and spatial dynamics of differentiation commitment in human epidermis. Elife 6:N/A (2017). PubMed: 29043977

客户评价及客户问答

Show All 评价 Q&A
提交评价 提交问题

Question

Hi, Can I get small trials of these products (ab122659 ab122548)? Thank you!

Read More
Answer

Thank you for contacting us. Because we carry over 90,000 products, it isn't feasible for us to keep small sample sizes of our products.

We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees that the product will work in the applications and species specified on the datasheet, or we will offer a replacement, credit, or refund within 6 months of purchase.

If the product is to be used in an untested species or application, you may be eligible for our AbTrial program if the antibody has not yet been purchased. Please contact our Scientific Support team by replying to this email prior to purchase for more information. Or you can find more information about this program at the following link:

www.abcam.com/abtrial

Otherwise, we like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Plus, each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates.

To find out more about our Abreview system, please see the following link:

https://www.abcam.com/abreviews

I hope this information is helpful. Please do not hesitate to contact us again with any other questions.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com