重组Anti-PPP6C/Ppv抗体[EPR8764] (ab131335)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8764] to PPP6C/Ppv
- Suitable for: Flow Cyt (Intra), WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-PPP6C/Ppv抗体[EPR8764]
参阅全部 PPP6C/Ppv 一抗 -
描述
兔单克隆抗体[EPR8764] to PPP6C/Ppv -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), WB, ICC/IFmore details
不适用于: IHC-P -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: HeLa, fetal thymus and 293T and Jurkat whole cell lysate (ab7899). ICC/IF: HeLa cells. Flow Cyt (intra): HeLa cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
解离常数(KD)
KD = 5.00 x 10 -11 M Learn more about KD -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EPR8764 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab131335于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt (Intra) |
1/280.
|
|
WB | (1) |
1/1000 - 1/10000. Predicted molecular weight: 35 kDa.
|
ICC/IF |
1/100 - 1/250.
|
说明 |
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Flow Cyt (Intra)
1/280. |
WB
1/1000 - 1/10000. Predicted molecular weight: 35 kDa. |
ICC/IF
1/100 - 1/250. |
靶标
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功能
Catalytic subunit of protein phospatase 6 (PP6). PP6 is a component of a signaling pathway regulating cell cycle progression in response to IL2 receptor stimulation. N-terminal domain restricts G1 to S phase progression in cancer cells, in part through control of cyclin D1. Downregulates MAP3K7 kinase activation of the IL1 signaling pathway by dephosphorylation of MAP3K7. -
组织特异性
Ubiquitously expressed in all tissues tested with highest expression levels in testis, heart, kidney, brain, stomach, liver and skeletal muscle and lowest in placenta, lung colon and spleen. -
序列相似性
Belongs to the PPP phosphatase family. PP-6 (PP-V) subfamily. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 5537 Human
- Entrez Gene: 67857 Mouse
- Entrez Gene: 171121 Rat
- Omim: 612725 Human
- SwissProt: O00743 Human
- SwissProt: Q9CQR6 Mouse
- SwissProt: Q64620 Rat
- Unigene: 728922 Human
see all -
别名
- FLJ92648 antibody
- MGC12249 antibody
- PP 6 antibody
see all
图片
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All lanes : Anti-PPP6C/Ppv antibody [EPR8764] (ab131335) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Fetal thymus tissue lysate
Lane 3 : 293T cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP conjugated at 1/2000 dilution
Predicted band size: 35 kDa -
Immunofluorescent analysis of HeLa cells labelling PPP6C/Ppv with ab131335 at 1/100 dilution.
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Intracellular Flow Cytometry analysis ofHeLa cells labelling PPP6C/Ppv with purified ab131335 at a dilution of 1/280 (red). Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. An Alexa Flour® 488-conjugated goat anti-rabbit IgG (1/2000) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab131335 被引用在 5 文献中.
- Sobajima T et al. PP6 regulation of Aurora A-TPX2 limits NDC80 phosphorylation and mitotic spindle size. J Cell Biol 222:N/A (2023). PubMed: 36897279
- Song TY et al. Tumor evolution selectively inactivates the core microRNA machinery for immune evasion. Nat Commun 12:7003 (2021). PubMed: 34853298
- Moffett HF et al. The microRNA miR-31 inhibits CD8+ T cell function in chronic viral infection. Nat Immunol 18:791-799 (2017). WB ; Mouse . PubMed: 28530712
- Yan S et al. NF-?B-induced microRNA-31 promotes epidermal hyperplasia by repressing protein phosphatase 6 in psoriasis. Nat Commun 6:7652 (2015). PubMed: 26138368
- Montoya V et al. Novel miRNA-31 and miRNA-200a-Mediated Regulation of Retinoblastoma Proliferation. PLoS One 10:e0138366 (2015). WB, IF . PubMed: 26379276