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AB198290

重组Anti-PPP2R5E抗体[EPR17146]

Anti-PPP2R5E antibody [EPR17146]

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(1 Publication)

Rabbit Recombinant Monoclonal 2A5E antibody. Suitable for WB, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 1 publication.

查看别名

Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit epsilon isoform, PP2A B subunit isoform B'-epsilon, PP2A B subunit isoform B56-epsilon, PP2A B subunit isoform PR61-epsilon, PP2A B subunit isoform R5-epsilon, PPP2R5E

6 Images
Immunocytochemistry/ Immunofluorescence - Anti-PPP2R5E antibody [EPR17146] (AB198290)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PPP2R5E antibody [EPR17146] (AB198290)

Immunofluorescent analysis of 100% methanol-fixed, 0.1% Triton X-100 permeabilized C6 (Rat glial tumor) cells labeling PPP2R5E with ab198290 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Cytoplasm staining on C6 cell line is observed. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows : -
-ve control 1 - ab198290 at 1/500 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
-ve control 2. - ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

Western blot - Anti-PPP2R5E antibody [EPR17146] (AB198290)
  • WB

Supplier Data

Western blot - Anti-PPP2R5E antibody [EPR17146] (AB198290)

Blocking/dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-PPP2R5E antibody [EPR17146] (ab198290) at 1/1000 dilution

All lanes:

Human pancreas lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 31 kDa,55 kDa

Observed band size: 30 kDa,55 kDa

false

Western blot - Anti-PPP2R5E antibody [EPR17146] (AB198290)
  • WB

Lab

Western blot - Anti-PPP2R5E antibody [EPR17146] (AB198290)

Lanes 1-4 : Merged signal (red and green). Green - ab198290 observed at 55 kDa. Red - loading control ab8245 observed at 37 kDa.

ab198290 Anti-PPP2R5E antibody [EPR17146] was shown to specifically react with PPP2R5E in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265637 (knockout cell lysate ab258135) was used. Wild-type and PPP2R5E knockout samples were subjected to SDS-PAGE. ab198290 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-PPP2R5E antibody [EPR17146] (ab198290) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

PPP2R5E knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human PPP2R5E knockout HeLa cell line (<a href='/products/cell-lines/human-ppp2r5e-knockout-hela-cell-line-ab265637'>ab265637</a>)

Lane 3:

HEK-293 cell lysate at 20 µg

Lane 4:

Daudi cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/20000 dilution

Predicted band size: 55 kDa

Observed band size: 55 kDa

false

Western blot - Anti-PPP2R5E antibody [EPR17146] (AB198290)
  • WB

Supplier Data

Western blot - Anti-PPP2R5E antibody [EPR17146] (AB198290)

Blocking/dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-PPP2R5E antibody [EPR17146] (ab198290) at 1/1000 dilution

All lanes:

293 (Human epithelial cells from embryonic kidney) cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 55 kDa

Observed band size: 55 kDa

false

Western blot - Anti-PPP2R5E antibody [EPR17146] (AB198290)
  • WB

Supplier Data

Western blot - Anti-PPP2R5E antibody [EPR17146] (AB198290)

Blocking/dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-PPP2R5E antibody [EPR17146] (ab198290) at 1/1000 dilution

All lanes:

Human fetal brain lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 55 kDa

Observed band size: 55 kDa

false

Western blot - Anti-PPP2R5E antibody [EPR17146] (AB198290)
  • WB

Supplier Data

Western blot - Anti-PPP2R5E antibody [EPR17146] (AB198290)

Blocking/dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-PPP2R5E antibody [EPR17146] (ab198290) at 1/1000 dilution

Lane 1:

Mouse brain lysate at 10 µg

Lane 2:

Rat brain lysate at 10 µg

Lane 3:

C6 (Rat glial tumor cell) lysate at 10 µg

Lane 4:

NIH/3T3 (Mouse embryo fibroblast cells) lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 55 kDa,61 kDa

Observed band size: 55 kDa

false

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR17146

亚型

IgG

不含载体蛋白

No

反应种属

Mouse, Rat, Human

应用

WB, ICC/IF

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p></p>" } } }
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产品详情

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The protein PPP2R5E also known as B56 epsilon is a regulatory subunit of protein phosphatase 2A (PP2A). It has an approximate mass of 72 kDa. PPP2R5E expresses across various tissues including the brain heart and skeletal muscle. The subunit associates with the core PP2A enzyme influencing substrate specificity and activity of PP2A holoenzyme.
Biological function summary

PPP2R5E plays a role in regulating cell growth and division. It is a part of the PP2A holoenzyme complex acting as a critical modulatory component. By associating with the PP2A core enzyme PPP2R5E contributes to the dephosphorylation process of many substrates. These substrates are vital for maintaining cellular functions such as signal transduction and cell cycle progression.

Pathways

PPP2R5E takes part in cell signaling and apoptotic pathways. It has substantial involvement in the MAPK signaling pathway where it modulates the activity of related kinases. PPP2R5E interacts with proteins like RAF1 influencing the pathway's function. Additionally it also participates in the Wnt signaling pathway where its regulatory role impacts cellular differentiation and proliferation.

PPP2R5E shows links to cancer and neurodegenerative diseases. Alterations in its expression or function may lead to oncogenesis due to its role in controlling cell proliferation signals. The dysregulation of PP2A activity potentially through PPP2R5E has associations with Alzheimer’s disease. In cancer PPP2R5E interacts with proteins such as c-Myc which contributes to tumorigenesis and progression.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment.
See full target information Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit epsilon isoform
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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Nature medicine 31:1604-1616 PubMed40050704

2025

Microglial mechanisms drive amyloid-β clearance in immunized patients with Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Lynn van Olst,Brooke Simonton,Alex J Edwards,Anne V Forsyth,Jake Boles,Pouya Jamshidi,Thomas Watson,Nate Shepard,Talia Krainc,Benney Mr Argue,Ziyang Zhang,Joshua Kuruvilla,Lily Camp,Mengwei Li,Hang Xu,Jeanette L Norman,Joshua Cahan,Robert Vassar,Jinmiao Chen,Rudolph J Castellani,James Ar Nicoll,Delphine Boche,David Gate
View all publications
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替代产品

Primary Antibodies

AB198500

Anti-PPP2R5E antibody [EPR17147] - C-terminal

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搭配使用产品

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  • 0
  • 0
Primary Antibodies

AB177935

Anti-PPA2 antibody [EPR13087(B)]

RabMAb|Recombinant

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  • 0
  • 0
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RabMAb|Recombinant|KO Validated|20ul selling size

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  • 3
  • 1

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