Anti-PPP2R5D抗体(ab72303)
Key features and details
- Rabbit polyclonal to PPP2R5D
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-PPP2R5D抗体
参阅全部 PPP2R5D 一抗 -
描述
兔多克隆抗体to PPP2R5D -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Human
预测可用于: Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Opossum, Orangutan, Elephant
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免疫原
Synthetic peptide corresponding to a region between residues 1 and 50 of human PPP2R5D (NP_006236.1).
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阳性对照
- Whole cell lysate from HeLa cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab72303于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) |
1/2000 - 1/10000. Detects a band of approximately 74 kDa (predicted molecular weight: 67 kDa).
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| IP |
Use at 2-5 µg/mg of lysate.
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| 说明 |
|---|
|
WB
1/2000 - 1/10000. Detects a band of approximately 74 kDa (predicted molecular weight: 67 kDa). |
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IP
Use at 2-5 µg/mg of lysate. |
靶标
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功能
The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment. -
组织特异性
Isoform Delta-2 is widely expressed. Isoform Delta-1 is highly expressed in brain. -
序列相似性
Belongs to the phosphatase 2A regulatory subunit B56 family. -
细胞定位
Cytoplasm. Nucleus. Nuclear in interphase, nuclear during mitosis. - Information by UniProt
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数据库链接
- Entrez Gene: 100134966 Cow
- Entrez Gene: 5528 Human
- Entrez Gene: 100134967 Pig
- Omim: 601646 Human
- SwissProt: Q14738 Human
- Unigene: 533308 Human
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别名
- 2A5D_HUMAN antibody
- B'delta antibody
- B56D antibody
see all
图片
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Western blot - Anti-PPP2R5D antibody (ab72303)All lanes : Anti-PPP2R5D antibody (ab72303) at 0.04 µg/ml
Lane 1 : Whole cell lysate from HeLa cells at 50 µg
Lane 2 : Whole cell lysate from HeLa cells at 15 µg
Lane 3 : Whole cell lysate from HeLa cells at 5 µg
Developed using the ECL technique.
Predicted band size: 67 kDa
Observed band size: 74 kDa why is the actual band size different from the predicted?
Exposure time: 10 seconds -
Immunoprecipitation - Anti-PPP2R5D antibody (ab72303)Detection of Human PPP2R5D by Immunoprecipitation in whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab72303 at 3 µg/mg lysate for IP and at 0.1 µg/ml for subsequent Western blot detection.
Detection: Chemiluminescence with an exposure time of 10 seconds. seconds
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab72303 尚未被引用在任何文献中。