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Neuroscience Neurotransmission Intracellular Signaling Phosphatases

Anti-PPP2R5B抗体(ab1366)

  • Datasheet
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Western blot - Anti-PPP2R5B antibody (ab1366)

    Key features and details

    • Goat polyclonal to PPP2R5B
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

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    概述

    • 产品名称

      Anti-PPP2R5B抗体
      参阅全部 PPP2R5B 一抗
    • 描述

      山羊多克隆抗体to PPP2R5B
    • 宿主

      Goat
    • 经测试应用

      适用于: WBmore details
    • 种属反应性

      与反应: Human
      预测可用于: Mouse, Rat, Dog
    • 免疫原

      Synthetic peptide: QRLTPQVAASGGQS, corresponding to C terminal amino acids 484-497 of Human PPP2R5B.

      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    • 常规说明

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    • 形式

      Liquid
    • 存放说明

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
    • 存储溶液

      pH: 7.30
      Preservative: 0.02% Sodium azide
      Constituents: Tris buffered saline, 0.5% BSA
    • Concentration information loading...
    • 纯度

      Immunogen affinity purified
    • 纯化说明

      Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    • 克隆

      多克隆
    • 同种型

      IgG
    • 研究领域

      • Neuroscience
      • Neurotransmission
      • Intracellular Signaling
      • Phosphatases
      • Signal Transduction
      • Protein Phosphorylation
      • Ser / Thr Phosphatases

    相关产品

    • Compatible Secondaries

      • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
      • Donkey Anti-Goat IgG H&L (HRP) (ab205723)
    • Isotype control

      • Goat IgG, polyclonal - Isotype Control (ab37373)

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab1366于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    WB
    说明
    应用说明
    ELISA: Detection limit dilution 1/2000.
    WB: Use at a concentration of 2 - 3 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    靶标

    • 功能

      The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment. The phosphorylated form mediates the interaction between AKT1 and PP2A phosphatase leading to dephosphorylation of AKT1 on the 'Thr-308' and 'Ser-373' residues.
    • 组织特异性

      Highest expression in brain.
    • 序列相似性

      Belongs to the phosphatase 2A regulatory subunit B56 family.
    • 细胞定位

      Cytoplasm.
    • Target information above from: UniProt accession Q15173 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 数据库链接

      • Entrez Gene: 5526 Human
      • Entrez Gene: 225849 Mouse
      • Entrez Gene: 309179 Rat
      • NCBI: 5453952 Human
      • Omim: 601644 Human
      • SwissProt: Q15173 Human
      • Unigene: 75199 Human
      • 别名

        • 2A5B_HUMAN antibody
        • B56B antibody
        • Beta isoform of regulatory subunit B56 protein phosphatase 2A antibody
        • FLJ35411 antibody
        • PP2A B subunit B' beta isoform antibody
        • PP2A B subunit B56 beta isoform antibody
        • PP2A B subunit isoform B' beta antibody
        • PP2A B subunit isoform B''-beta antibody
        • PP2A B subunit isoform B56-beta antibody
        • PP2A B subunit isoform PR61-beta antibody
        • PP2A B subunit isoform R5-beta antibody
        • PP2A B subunit PR61 beta isoform antibody
        • PP2A B subunit R5 beta isoform antibody
        • PPP2R5B antibody
        • PR 61B antibody
        • PR61B antibody
        • Protein phosphatase 2 regulatory subunit B (B56) beta antibody
        • Protein phosphatase 2 regulatory subunit B (B56) beta isoform antibody
        • Protein phosphatase 2 regulatory subunit B' beta antibody
        • Protein phosphatase 2 regulatory subunit B' beta isoform antibody
        • Serine/threonine protein phosphatase 2A 56 kDa regulatory subunit beta isoform antibody
        • Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit beta isoform antibody
        see all

      图片

      • Western blot - Anti-PPP2R5B antibody (ab1366)
        Western blot - Anti-PPP2R5B antibody (ab1366)
        Anti-PPP2R5B antibody (ab1366) at 2 µg/ml + Human cerebellum lysate at 35 µg

        Predicted band size: 55 kDa
        Observed band size: 55 kDa

      实验方案

      • Western blot protocols

      Click here to view the general protocols

      数据表及文件

      • Datasheet download

        Download

      文献 (1)

      发表研究结果有使用 ab1366?请让我们知道,以便我们可以引用本数据表中的参考文章。

      ab1366 被引用在 1 文献中.

      • DeGrande ST  et al. Molecular mechanisms underlying cardiac protein phosphatase 2A regulation in heart. J Biol Chem 288:1032-46 (2013). WB ; Human, Mouse, Rat, Dog . PubMed: 23204520

      客户评价及客户问答

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      Immunocytochemistry/ Immunofluorescence abreview for Anti-PPP2R5B antibody

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Human Cell (HEK293T)
      Permeabilization
      Yes - 0.1% Triton X-100
      Specification
      HEK293T
      Blocking step
      Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
      Fixative
      Formaldehyde
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      提交于 Mar 31 2017

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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