重组Anti-Ppp1r17抗体[EPR29124-227]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ppp1r17 antibody [EPR29124-227] (AB317831)

Immunohistochemical analysis of paraffin-embedded Mouse cerebellum tissue labeling Ppp1r17 with ab317831 at 1/2000 (0.262 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on mouse cerebellum (PMID : 9920894, 22340725).
The section was incubated with ab317831 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ppp1r17 antibody [EPR29124-227] (AB317831)

Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling Ppp1r17 with ab317831 at 1/2000 (0.262 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : no staining on mouse liver.
The section was incubated with ab317831 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Anti-Ppp1r17 antibody [EPR29124-227] (AB317831)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized Mouse liver (perfused-fixed) tissue labeling Ppp1r17 with ab317831 at a 1/500 (1.046 µg/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at a 1/1000 (2 µg/mL) dilution.

Negative control : confocal image showing no staining on mouse liver. The nuclear counterstain was DAPI (Blue). The section was incubated with ab317831 for 60 mins at room temperature. The section was then mounted using Fluoromount^®.The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20)

• IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Anti-Ppp1r17 antibody [EPR29124-227] (AB317831)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized Rat liver (perfused-fixed) tissue labeling Ppp1r17 with ab317831 at a 1/500 (1.046 µg/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at a 1/1000 (2 µg/mL) dilution.

Negative control : confocal image showing no staining on rat liver. The nuclear counterstain was DAPI (Blue). The section was incubated with ab317831 for 60 mins at room temperature. The section was then mounted using Fluoromount^®.The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20)

• IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Anti-Ppp1r17 antibody [EPR29124-227] (AB317831)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized Rat cerebellum (perfused-fixed) tissue labeling Ppp1r17 with ab317831 at a 1/500 (1.046 µg/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at a 1/1000 (2 µg/mL) dilution.

Panel A : merged staining of anti-Ppp1r17 (ab317831, green), anti-NeuN (ab190565, grey) and anti-GFAP (ab201732, magenta) on rat cerebellum.
Panel B : anti-Ppp1r17 stained on rat cerebellum.
Panel C : anti-NeuN stained in neurons of rat cerebellum.
Panel D : anti-GFAP stained in astrocytes of rat cerebellum.

The section was incubated in two rounds of staining : in the order of ab317831 and ab190565, ab201732 for 1 hr at room temperature. The nuclear counterstain was DAPI (Blue). The section was then mounted using Fluoromount^®.The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20)

• IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Anti-Ppp1r17 antibody [EPR29124-227] (AB317831)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized Mouse cerebellum (perfused-fixed) tissue labeling Ppp1r17 with ab317831 at a 1/500 (1.046 µg/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at a 1/1000 (2 µg/mL) dilution.

Panel A : merged staining of anti-Ppp1r17 (ab317831, green), anti-NeuN (ab190565, grey) and anti-GFAP (ab201732, magenta) on mouse cerebellum.
Panel B : anti-Ppp1r17 stained on mouse cerebellum.
Panel C : anti-NeuN stained in neurons of mouse cerebellum.
Panel D : anti-GFAP stained in astrocytes of mouse cerebellum.

The section was incubated in two rounds of staining : in the order of ab317831 and ab190565, ab201732 for 1 hr at room temperature. The nuclear counterstain was DAPI (Blue). The section was then mounted using Fluoromount^®.The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20)

• mIHC

Lab

Multiplex immunohistochemistry - Anti-Ppp1r17 antibody [EPR29124-227] (AB317831)

Multiplex immunohistochemistry analysis of Formalin/PFA-fixed paraffin-embedded sections of Rat cerebellum tissue staining with ab317831 at 1/2000 dilution, ab313624 at 1/4000 dilution, ab307710 at 1/2000 dilution. Secondary used was Opal Polymer HRP Ms + Rb. Panel A : merged staining of anti-Ppp1r17 (magenta; Opal™690), anti-KCNA2 (green; Opal™520) and anti-KCND2 (gray; Opal™570) on rat cerebellum. Panel B : anti-Ppp1r17 staining the Purkinje cells in rat cerebellum. Panel C : anti-KCNA2 staining the basket cells in rat cerebellum. Panel D : anti-KCND2 staining the granule cell layer in rat cerebellum. Nuclear DNA was labeled with DAPI (shown in blue). The section was incubated in three rounds of staining : in the order of ab317831, ab313624 and ab307710 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-Ppp1r17 antibody [EPR29124-227] (AB317831)

Multiplex immunohistochemistry analysis of Formalin/PFA-fixed paraffin-embedded sections of Mouse cerebellum tissue staining with ab317831 at 1/2000 dilution, ab313624 at 1/4000 dilution, ab307710 at 1/2000 dilution. Secondary used was Opal Polymer HRP Ms + Rb. Panel A : merged staining of anti-Ppp1r17 (magenta; Opal™690), anti-KCNA2 (green; Opal™520) and anti-KCND2 (gray; Opal™570) on mouse cerebellum. Panel B : anti-Ppp1r17 staining the Purkinje cells in mouse cerebellum. Panel C : anti-KCNA2 staining the basket cells in mouse cerebellum. Panel D : anti-KCND2 staining the granule cell layer in mouse cerebellum. Nuclear DNA was labeled with DAPI (shown in blue). The section was incubated in three rounds of staining : in the order of ab317831, ab313624 and ab307710 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ppp1r17 antibody [EPR29124-227] (AB317831)

Immunohistochemical analysis of paraffin-embedded Rat cerebellum tissue labeling Ppp1r17 with ab317831 at 1/2000 (0.262 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on rat cerebellum.
The section was incubated with ab317831 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ppp1r17 antibody [EPR29124-227] (AB317831)

Immunohistochemical analysis of paraffin-embedded Mouse whole brain (sagittal plane) tissue labeling Ppp1r17 with ab317831 at 1/2000 (0.262 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on cerebellum and hypothalamus of mouse whole brain section (PMID : 9920894, 22340725, 33753517).
The section was incubated with ab317831 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ppp1r17 antibody [EPR29124-227] (AB317831)

Immunohistochemical analysis of paraffin-embedded Rat hypothalamus tissue labeling Ppp1r17 with ab317831 at 1/2000 (0.262 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on rat hypothalamus.
The section was incubated with ab317831 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ppp1r17 antibody [EPR29124-227] (AB317831)

Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling Ppp1r17 with ab317831 at 1/2000 (0.262 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : no staining on rat liver.
The section was incubated with ab317831 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ppp1r17 antibody [EPR29124-227] (AB317831)

Immunohistochemical analysis of paraffin-embedded Mouse hypothalamus tissue labeling Ppp1r17 with ab317831 at 1/2000 (0.262 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on mouse hypothalamus (PMID : 9920894, 22340725, 33753517).
The section was incubated with ab317831 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IP

Supplier Data

Immunoprecipitation - Anti-Ppp1r17 antibody [EPR29124-227] (AB317831)

Ppp1r17 was immunoprecipitated from 0.35 mg Mouse cerebellum tissue lysate with ab317831 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab317831 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : Mouse cerebellum tissue lysate
Lane 2 : ab317831 IP in Mouse cerebellum tissue lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab317831 in Mouse cerebellum tissue lysate

All lanes:

Immunoprecipitation - Anti-Ppp1r17 antibody [EPR29124-227] (ab317831) at 1/30 dilution

All lanes:

Mouse cerebellum tissue lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-Ppp1r17 antibody [EPR29124-227] (AB317831)

Negative control : cerebral cortex, liver, lung.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-Ppp1r17 antibody [EPR29124-227] (ab317831) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate at 20 µg with NFDM/TBST

Lane 2:

Mouse cerebellum tissue lysate at 20 µg with NFDM/TBST

Lane 3:

Mouse cerebral cortex tissue lysate at 20 µg with NFDM/TBST

Lane 4:

Mouse liver tissue lysate at 20 µg with NFDM/TBST

Lane 5:

Mouse lung tissue lysate at 20 µg with NFDM/TBST

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 23 kDa,36 kDa

true

Exposure time: 92s

• WB

Supplier Data

Western blot - Anti-Ppp1r17 antibody [EPR29124-227] (AB317831)

Negative control : cerebral cortex, liver, spleen.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-Ppp1r17 antibody [EPR29124-227] (ab317831) at 1/1000 dilution

Lane 1:

Rat cerebellum tissue lysate at 20 µg with NFDM/TBST

Lane 2:

Rat cerebral cortex tissue lysate at 20 µg with NFDM/TBST

Lane 3:

Rat liver tissue lysate at 20 µg with NFDM/TBST

Lane 4:

Rat spleen tissue lysate at 20 µg with NFDM/TBST

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 23 kDa,36 kDa

true

Exposure time: 180s