重组Anti-PPP1CB抗体[EP1804Y] (ab53315)

Blocking and diluting buffer: 5% NFDM/TBST

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human cervical carcinoma tissue sections labeling PPP1CB with purified ab53315 at 1/400 dilution (1 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, pH 9.0. Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used. PBS instead of the primary antibody was used as the negative control.

Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Protein phosphatase 1 beta with purified ab53315 at 1/40 dilution (10 ug/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluorr^® 488) secondary antibody was used at 1/2000 dilution. Isotype control - 90% methanol. Unlabeled control - Rabbit monoclonal IgG (Black). Cell without incubation with primary antibody and secondary antibody (Blue).

ab53315 (purified) at 1/100 dilution (2ug) immunoprecipitating Protein phosphatase 1 beta in Human fetal brain lysate.

Lane 1 (input): Human fetal brain lysate 10ug

Lane 2 (+): ab53315 & Human fetal brain lysate

Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab53315 in Human fetal brain lysate

For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/1000 dilution.

Blocking and diluting buffer: 5% NFDM/TBST.

Unpurified ab53315 staining PPP1CB antibody in HeLa (human cervix adenocarcinoma) cells by ICC (Immunocytochemistry). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at 1/1000 dilution.

DAPI was used as a nuclear counterstain and the negative control was PBS only.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat kidney tissue sections labeling PPP1CB with purified ab53315 at 1/400 dilution (1 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, pH 9.0. Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used. PBS instead of the primary antibody was used as the negative control.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse kidney tissue sections labeling PPP1CB with purified ab53315 at 1/400 dilution (1 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, pH 9.0. Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used. PBS instead of the primary antibody was used as the negative control.

Immunocytochemistry analysis of MCF-7 (Human breast adenocarcinoma epithelial cell) cells labeling PPP1CB with Purified ab53315 at 1:250 dilution (1.6 μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 μg/ml). ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) was used as the secondary antibody at 1/1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

Immunohistochemical analysis of paraffin-embedded sections of Human cervical carcinoma labelling PPP1CB with unpurified ab53315 at 1/250 dilution. Prediluted ImmunoHistoprobe (ready to use) HRP Polymer for Rabbit IgG was used as a secondary antibody.

Counter stain = Hematoxylin. Heat mediated antigen retrieval using EDTA buffer at pH 9 was performed.

Immunohistochemical analysis of paraffin-embedded sections of mouse cardiac muscle labelling PPP1CB with unpurified ab53315 at 1/250 dilution. Prediluted ImmunoHistoprobe (ready to use) HRP Polymer for Rabbit IgG was used as a secondary antibody.

Counter stain = Hematoxylin. Heat mediated antigen retrieval using EDTA buffer at pH 9 was performed.

Immunohistochemical analysis of paraffin-embedded sections of rat colon labelling PPP1CB with unpurified ab53315 at 1/250 dilution. Prediluted ImmunoHistoprobe (ready to use) HRP Polymer for Rabbit IgG was used as a secondary antibody.

Counter stain = Hematoxylin. Heat mediated antigen retrieval using EDTA buffer at pH 9 was performed.

Flow Cytometry (Intracellular) analysis of HeLa cells labelling PPP1CB with unpurified ab53315 at 1/20 dilution. Goat anti rabbit IgG (H&L) (FITC) at 1/150 dilution was used as the secondary antibody. Isotype control = Rabbit monoclonal IgG. Fixative = 2% paraformaldehyde.