重组Anti-PPP1CA + PPP1CB抗体[EP1511Y] (ab52619)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1511Y] to PPP1CA + PPP1CB
- Suitable for: Flow Cyt (Intra), WB, IP, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-PPP1CA + PPP1CB抗体[EP1511Y]
参阅全部 PPP1CA + PPP1CB 一抗 -
描述
兔单克隆抗体[EP1511Y] to PPP1CA + PPP1CB -
宿主
Rabbit -
特异性
The immunogen for ab52619 is 100% homologous with Human PPP1CA and PPP1CB -
经测试应用
适用于: Flow Cyt (Intra), WB, IP, IHC-P, ICC/IFmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human PPP1CA + PPP1CB aa 200-300. The exact sequence is proprietary.
Database link: P62140 -
阳性对照
- WB: mouse brain lysate, rat brain lysate; T47-D cell lysate, HeLa cell lysate; SKBR-3 cell lysate, Jurkat cell lysate. ICC/IF: HepG2 cells. IHC-P: Human cerebral cortex tissue. Flow Cyt: HeLa cells. IP: Jurkat cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP1511Y -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab52619于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Flow Cyt (Intra) |
1/100 - 1/150.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB | (1) |
1/20000 - 1/100000. Predicted molecular weight: 37 kDa.
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IP |
1/30 - 1/100.
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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ICC/IF |
1/50 - 1/250.
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说明 |
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Flow Cyt (Intra)
1/100 - 1/150. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/20000 - 1/100000. Predicted molecular weight: 37 kDa. |
IP
1/30 - 1/100. |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ICC/IF
1/50 - 1/250. |
靶标
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细胞定位
Cytoplasm. Nucleus. Nucleus > nucleoplasm. Nucleus > nucleolus. -
数据库链接
- Entrez Gene: 5499 Human
- Entrez Gene: 5500 Human
- Entrez Gene: 19045 Mouse
- Entrez Gene: 19046 Mouse
- Entrez Gene: 24668 Rat
- Entrez Gene: 25594 Rat
- Omim: 176875 Human
- Omim: 600590 Human
see all -
别名
- PP-1A antibody
- PP-1B antibody
- PP1A antibody
see all
图片
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ab52619 staining PPP1CA + 1CB in the HepG2 cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody (1/50). ab150077(1/500) an Alexa Fluor®488-conjugated Goat anti-rabbit IgG was used as the secondary antibody. Nuclei were counterstained with DAPI.
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All lanes : Anti-PPP1CA + PPP1CB antibody [EP1511Y] (ab52619) at 1/100000 dilution
Lane 1 : Mouse Brain lysate
Lane 2 : Rat Brain lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), HRP-conjugated at 1/1000 dilution
Predicted band size: 37 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPP1CA + PPP1CB antibody [EP1511Y] (ab52619)
ab52619 staining PP1CA + 1CB in Human cerebrum cortex tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed and paraffin-embedded, antigen retrieval was by heat mediation in Tris/EDTA buffer pH9. Samples were incubated with primary antibody (1/50). An undiluted HRP-conjugated mouse anti-rabbit IgG was used as the secondary antibody. Tissue counterstained with Hematoxylin. PBS was used in the negative control rather than the Primary antibody.
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Overlay histogram showingHeLa cells stained with ab52619 (red line) at 1/150 dilution. The cells were fixed with 80% methanol. The secondary antibody used was a FITC conjugated goat anti-rabbit IgG at 1/150 dilution. Isotype control antibody (black line) was rabbit monoclonal IgG used under the same conditions. Cells also incubated without primary antibody and secondary antibody (blue line)
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ab52619 (purified) at 1/30 immunoprecipitating PPP1CA + 1CB in Jurkat cell lysate. For western blotting, a HRP-conjugated Goat anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1000).
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
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All lanes : Anti-PPP1CA + PPP1CB antibody [EP1511Y] (ab52619) at 1/100000 dilution
Lane 1 : T47-D cell Lysate
Lane 2 : HeLa cell Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), HRP-conjugated at 1/1000 dilution
Predicted band size: 37 kDa -
Overlay histogram showing HeLa cells stained with ab52619, unpurified (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab52619, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
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All lanes : Anti-PPP1CA + PPP1CB antibody [EP1511Y] (ab52619) at 1/20000 dilution
Lane 1 : SKBR-3 cell lysate
Lane 2 : Jurkat cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), HRP-conjugated at 1/1000 dilution
Predicted band size: 37 kDa
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (6)
ab52619 被引用在 6 文献中.
- Kim JE et al. CDDO-Me Selectively Attenuates CA1 Neuronal Death Induced by Status Epilepticus via Facilitating Mitochondrial Fission Independent of LONP1. Cells 8:N/A (2019). PubMed: 31387295
- Kim JE et al. Blockade of AMPA Receptor Regulates Mitochondrial Dynamics by Modulating ERK1/2 and PP1/PP2A-Mediated DRP1-S616 Phosphorylations in the Normal Rat Hippocampus. Front Cell Neurosci 13:179 (2019). PubMed: 31118889
- Nagarajan S et al. Protein Phosphatase 1a enhances renal aldosterone signaling via mineralocorticoid receptor stabilization. Mol Cell Endocrinol 450:74-82 (2017). PubMed: 28454724
- Pribiag H & Stellwagen D TNF-a downregulates inhibitory neurotransmission through protein phosphatase 1-dependent trafficking of GABA(A) receptors. J Neurosci 33:15879-93 (2013). WB . PubMed: 24089494
- Horiuchi D et al. MYC pathway activation in triple-negative breast cancer is synthetic lethal with CDK inhibition. J Exp Med 209:679-96 (2012). PubMed: 22430491
- Kuehnen P et al. Protein phosphatase 1 (PP-1)-dependent inhibition of insulin secretion by leptin in INS-1 pancreatic ß-cells and human pancreatic islets. Endocrinology 152:1800-8 (2011). PubMed: 21427225