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RabMAb

Anti-PPP1A/PPP1CA (phospho T320)抗体[EP1512Y] (ab62334)

Submit a review Q&A (1)References (23)
Western blot - Anti-PPP1A/PPP1CA (phospho T320) antibody [EP1512Y] (ab62334)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPP1A/PPP1CA (phospho T320) antibody [EP1512Y] (ab62334)

Key features and details

  • Rabbit monoclonal [EP1512Y] to PPP1A/PPP1CA (phospho T320)
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

Conjugates logo Related conjugates and formulations

Carrier Free

概述

  • 产品名称

    Anti-PPP1A/PPP1CA (phospho T320)抗体[EP1512Y]
    参阅全部 PPP1A/PPP1CA 一抗

  • 描述

    兔单克隆抗体[EP1512Y] to PPP1A/PPP1CA (phospho T320)

  • 宿主

    Rabbit

  • 特异性

    This antibody detects PP1 alpha phosphorylated at Threonine 320.

  • 经测试应用

    适用于: WB, IHC-Pmore details
    不适用于: Flow Cyt or ICC/IF

  • 种属反应性

    与反应: Human
    预测可用于: Mouse, Rat, Xenopus laevis

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • WB: HeLa cell lysate. IHC-P: Human muscle tissue.

  • 常规说明

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab62334于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
1/1000 - 1/10000. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa).
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
说明
WB
1/1000 - 1/10000. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa).
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
应用说明
Is unsuitable for Flow Cyt or ICC/IF.

靶标

  • 功能

    Protein phosphatase 1 (PP1) is essential for cell division, and participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. Involved in regulation of ionic conductances and long-term synaptic plasticity. May play an important role in dephosphorylating substrates such as the postsynaptic density-associated Ca(2+)/calmodulin dependent protein kinase II. Component of the PTW/PP1 phosphatase complex, which plays a role in the control of chromatin structure and cell cycle progression during the transition from mitosis into interphase.

  • 序列相似性

    Belongs to the PPP phosphatase family. PP-1 subfamily.

  • 细胞定位

    Cytoplasm. Nucleus. Nucleus > nucleoplasm. Nucleus > nucleolus. Primarily nuclear and largely excluded from the nucleolus. Highly mobile in cells and can be relocalized through interaction with targeting subunits. NOM1 plays a role in targeting this protein to the nucleolus. In the presence of PPP1R8 relocalizes from the nucleus to nuclear speckles.
  • Information by UniProt

  • 数据库链接

    see all

  • 别名

    • Alpha isoform serine threonine protein phosphatase PP1alpha 1 catalytic subunit antibody
    • Catalytic subunit antibody
    • EC 3.1.3.16 antibody
    • MGC15877 antibody
    • MGC1674 antibody
    • PP 1A antibody
    • PP-1A antibody
    • PP1A antibody
    • PP1A_HUMAN antibody
    • PP1alpha antibody
    • PP2C ALPHA antibody
    • PP2CA antibody
    • Ppp1ca antibody
    • Protein Phosphatase 2C Alpha Isoform antibody
    • Serine threonine protein phosphatase PP1 alpha catalytic subunit antibody
    • Serine threonine protein phosphatase PP1 alpha catalytic subunit protein phosphatase 1 antibody
    • Serine/threonine-protein phosphatase PP1-alpha catalytic subunit antibody
    see all

图片

  • Western blot - Anti-PPP1A/PPP1CA (phospho T320) antibody [EP1512Y] (ab62334)
    Western blot - Anti-PPP1A/PPP1CA (phospho T320) antibody [EP1512Y] (ab62334)
    All lanes : Anti-PPP1A/PPP1CA (phospho T320) antibody [EP1512Y] (ab62334)

    Lane 1 : HeLa cell lysate (untreated)
    Lane 2 : HeLa cell lysate (treated with Calyculin A)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP conjugate at 1/2000 dilution

    Predicted band size: 38 kDa
    Observed band size: 38 kDa



    The bottom image shows beta Tubulin as a positive control.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPP1A/PPP1CA (phospho T320) antibody [EP1512Y] (ab62334)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPP1A/PPP1CA (phospho T320) antibody [EP1512Y] (ab62334)

    ab62334 (1/100 - 1/250 dilution) staining human PPP1A/PPP1CA in human muscle tissue by immunohistochemistry using paraffin embedded tissue.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

文献 (23)

发表研究结果有使用 ab62334？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab62334 被引用在 23 文献中.

  • Li J  et al. ODF2L acts as a synthetic lethal partner with WEE1 inhibition in epithelial ovarian cancer models. J Clin Invest 133:N/A (2023). PubMed: 36378528
  • Takahashi N  et al. CPEB1-dependent disruption of the mRNA translation program in oocytes during maternal aging. Nat Commun 14:416 (2023). PubMed: 36697412
  • Sobajima T  et al. PP6 regulation of Aurora A-TPX2 limits NDC80 phosphorylation and mitotic spindle size. J Cell Biol 222:N/A (2023). PubMed: 36897279
  • Sokhi S  et al. Myt1 overexpression mediates resistance to cell cycle and DNA damage checkpoint kinase inhibitors. Front Cell Dev Biol 11:1270542 (2023). PubMed: 38020882
  • Tavernier N  et al. Bora phosphorylation substitutes in trans for T-loop phosphorylation in Aurora A to promote mitotic entry. Nat Commun 12:1899 (2021). PubMed: 33771996
View all Publications for this product

客户评价及客户问答

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Question

I would like to test this antibody in WB with tissues from rabbit, an untested species.

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Answer

I am very pleased to hear you would like to test ab62334 in rabbit. This code will give you: 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview forrabbitand include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active. For more information on how to submit an Abreview, please visit the site: https://www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.

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