AB249536

重组Anti-PPOX抗体[EPR10400] - BSA and Azide free

Anti-PPOX antibody [EPR10400] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal PPOX antibody. Carrier free. Suitable for ICC/IF, WB, Flow Cyt (Intra) and reacts with Human samples.

查看别名

Protoporphyrinogen oxidase, PPO, PPOX

3 Images

Flow Cytometry (Intracellular) - Anti-PPOX antibody [EPR10400] - BSA and Azide free (AB249536)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-PPOX antibody [EPR10400] - BSA and Azide free (AB249536)

This data was developed using ab170412, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of permeabilized HT-1080 cells using ab170412 (red) at a 1/100 dilution or a rabbit IgG (negative) (green).

Immunocytochemistry/ Immunofluorescence - Anti-PPOX antibody [EPR10400] - BSA and Azide free (AB249536)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PPOX antibody [EPR10400] - BSA and Azide free (AB249536)

This data was developed using ab170412, the same antibody clone in a different buffer formulation.

Immunocytochemistry/ Immunofluorescence analysis of A549 cells labeling PPOX with ab170412 at a 1/100 dilution (green). DAPI nuclear staining (blue).

Supplier Data

Western blot - Anti-PPOX antibody [EPR10400] - BSA and Azide free (AB249536)

This data was developed using ab170412, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-PPOX antibody [EPR10400] (<a href='/products/primary-antibodies/ppox-antibody-epr10400-ab170412'>ab170412</a>) at 1/1000 dilution

Lane 1:

A549 cell lysate at 10 µg

Lane 2:

HepG2 cell lysate at 10 µg

Lane 3:

HT-1080 cell lysate at 10 µg

Lane 4:

293T (Human embryonic kidney epithelial cell) cell lysate at 10 µg

Predicted band size: 51 kDa

false

不同偶联物与剂型 (1)

Unconjugated

Anti-PPOX antibody [EPR10400]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR10400

亚型

IgG

不含载体蛋白

Yes

反应种属

Human

应用

ICC/IF, WB, Flow Cyt (Intra)

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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产品详情

ab249536 is the carrier-free version of ab170412.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification

存储溶液

pH: 7.2 - 7.4 Constituents: PBS

运输条件

Blue Ice

储存信息

Do Not Freeze

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

PPOX also known as protoporphyrinogen oxidase is an important enzyme in heme biosynthesis. It catalyzes the oxidation of protoporphyrinogen IX to protoporphyrin IX. The mass of PPOX is about 50 kDa and it is expressed in the mitochondria of cells across various tissues including the liver and bone marrow. PPOX's activity is important for producing heme which is an important component of hemoglobin.

Biological function summary

The enzyme PPOX contributes to heme production working within the mitochondrial membrane. It is part of the heme biosynthetic pathway and functions in converting protoporphyrinogen IX the last precursor to heme. This enzymatic action is important for producing heme groups required for hemoglobin myoglobin cytochromes and other heme-containing proteins. Without PPOX cells could not efficiently synthesize these important proteins.

Pathways

PPOX plays a role in the protoporphyrin IX biosynthesis pathway which forms part of the larger heme biosynthesis pathway. It interacts with other enzymes like coproporphyrinogen oxidase (CPOX) and ferrochelatase (FECH) which work sequentially in the pathway. These interactions ensure the completed heme molecule for functions like oxygen transport and electron transfer.

Defects in PPOX are linked to variegate porphyria a type of porphyria causing skin and neurological symptoms. Mutations in the PPOX gene reduce enzyme activity leading to the accumulation of protoporphyrinogen IX. This disease connects PPOX with other enzymes in the heme pathway such as hydroxymethylbilane synthase (HMBS) which is involved in acute intermittent porphyria. Proper understanding of PPOX and its pathway interactions helps in managing and diagnosing such conditions.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Catalyzes the 6-electron oxidation of protoporphyrinogen-IX to form protoporphyrin-IX.

See full target information PPOX

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Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

重组Anti-PPOX抗体[EPR10400] - BSA and Azide free